Differential Protein Expression of the Inhibitory Effects of a Standardized Extract fromScutellariae radixin Liver Cancer Cell Lines Using Liquid Chromatography and Tandem Mass Spectrometry

2005 ◽  
Vol 53 (1) ◽  
pp. 8-16 ◽  
Author(s):  
Eng Shi Ong ◽  
Shea Mei Len ◽  
Audrey Chee Huay Lee
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Liver Cancer ◽  
Tandem Mass Spectrometry ◽  
Protein Expression ◽  
Tandem Mass ◽  
Liver Cancer Cell ◽  
Inhibitory Effects ◽  
Differential Protein Expression ◽  
Differential Protein

scholarly journals A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Cells Cultured in DMEM 10% FBS and Chemically Defined Medium Using Human Adipose-Derived Mesenchymal Stem Cells

2018 ◽  
Vol 19 (7) ◽  
pp. 2042 ◽  
Author(s):  
Yoshiki Nakashima ◽  
Saifun Nahar ◽  
Chika Miyagi-Shiohira ◽  
Takao Kinjo ◽  
Naoya Kobayashi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Protein Expression ◽  
Defined Medium ◽  
Tandem Mass ◽  
Chemically Defined Medium ◽  
Biological Regulation

Human adipose-derived mesenchymal stem cells (hADSCs) are representative cell sources for cell therapy. Classically, Dulbecco’s Modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) has been used as culture medium for hADSCs. A chemically defined medium (CDM) containing no heterologous animal components has recently been used to produce therapeutic hADSCs. However, how the culture environment using a medium without FBS affects the protein expression of hADSC is unclear. We subjected hADSCs cultured in CDM and DMEM (10% FBS) to a protein expression analysis by tandem mass spectrometry liquid chromatography and noted 98.2% agreement in the proteins expressed by the CDM and DMEM groups. We classified 761 proteins expressed in both groups by their function in a gene ontology analysis. Thirty-one groups of proteins were classified as growth-related proteins in the CDM and DMEM groups, 16 were classified as antioxidant activity-related, 147 were classified as immune system process-related, 557 were involved in biological regulation, 493 were classified as metabolic process-related, and 407 were classified as related to stimulus responses. These results show that the trend in the expression of major proteins related to the therapeutic effect of hADSCs correlated strongly in both groups.

scholarly journals Untargeted Metabolomics Combined with Bioassay Reveals the Change in Critical Bioactive Compounds during the Processing of Qingzhuan Tea

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6718
Author(s):  
Peng-Cheng Zheng ◽  
Chun-Yin Qin ◽  
Pan-Pan Liu ◽  
Lin Feng ◽  
Tie-Jun Ling ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Correlation Analysis ◽  
Tandem Mass Spectrometry ◽  
Untargeted Metabolomics ◽  
Flavonoid Glycosides ◽  
Tandem Mass ◽  
Inhibitory Effects ◽  
Long Time ◽  
Metabolomics Analysis

Qingzhuan tea (QZT) is a typical Chinese dark tea that has a long-time manufacturing process. In the present study, liquid chromatography coupled with tandem mass spectrometry was used to study the chemical changes of tea samples during QZT processing. Untargeted metabolomics analysis revealed that the pile-fermentation and turnover (post-fermentation, FT) was the crucial stage in transforming the main compounds of QZT, whose contents of flavan-3-ols and flavonoids glycosides were decreased significantly. The bioactivities, including the antioxidant capacities and inhibitory effects on α-amylase and α-glucosidase, were also reduced after the FT process. It was suggested that although the QZT sensory properties improved following pile-fermentation and aging, the bioactivities remained restrained. Correlation analysis indicated that the main galloylated catechins and flavonoid glycosides were highly related to their antioxidant capacity and inhibitory effects on α-amylase and α-glucosidase.

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