New Method of DNA Isolation from Two Food Additives Suitable for Authentication in Polymerase Chain Reaction Assays

2005 ◽  
Vol 53 (9) ◽  
pp. 3345-3347 ◽  
Author(s):  
Mercedes Urdiaín ◽  
Antonio Doménech-Sánchez ◽  
Sebastián Albertí ◽  
V. Javier Benedí ◽  
Josep A. Rosselló
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Isolation ◽  
Food Additives ◽  
New Method ◽  
Chain Reaction ◽  
Polymerase Chain

Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean

2007 ◽  
Vol 42 (10) ◽  
pp. 1249-1255 ◽  
Author(s):  
Cibele dos Santos Ferrari ◽  
Luciana Lehmkuhl Valente ◽  
Fábio Cristiano Angonesi Brod ◽  
Caroline Tagliari ◽  
Ernani Sebastião Sant'Anna ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Isolation ◽  
Genetically Modified ◽  
Chain Reaction ◽  
Genetically Modified Soybean ◽  
Polymerase Chain

DNA Isolation from Recalcitrant Materials Such as Tree Roots, Bark, and Forest Soil for the Detection of Fungal Pathogens by Polymerase Chain Reaction

1998 ◽  
Vol 262 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Günther Bahnweg ◽  
Steffen Schulze ◽  
Evelyn M. Möller ◽  
Hilkea Rosenbrock ◽  
Christian Langebartels ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Forest Soil ◽  
Fungal Pathogens ◽  
Dna Isolation ◽  
Chain Reaction ◽  
Tree Roots ◽  
Polymerase Chain

A New Method for FMR1 Gene Methylation Screening by Multiplex Methylation-Specific Real-Time Polymerase Chain Reaction

2011 ◽  
Vol 15 (6) ◽  
pp. 387-393 ◽  
Author(s):  
Marjanu Hikmah Elias ◽  
Ravindran Ankathil ◽  
Abdul Razak Salmi ◽  
Wanna Sudhikaran ◽  
Pornprot Limprasert ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
New Method ◽  
Fmr1 Gene ◽  
Gene Methylation ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Method Comparison of DNA Isolation and Quantification for Fish and Seafood Authenticity Determination

2018 ◽  
Vol 13 (3) ◽  
pp. 115
Author(s):  
Dwiyitno Dwiyitno ◽  
Stefan Hoffman ◽  
Koen Parmentier ◽  
Chris Van Keer
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Dna Isolation ◽  
Blue Mussel ◽  
Pcr Amplification ◽  
Amplification Efficiency ◽  
Chain Reaction ◽  
Seafood Products ◽  
Polymerase Chain ◽  
Commercial Kit

Fish and seafood products has been commonly targeted for fraudulent activities. For that reason, authentication of fish and seafood products is important to protect consumers from fraudulent and adulteration practices, as well as to implement traceability regulation. From the viewpoint of food safety, authenticity is beneficial to protect public from serious food poisoning incidents, such as due to ingestion of toxic species. Since DNA based identification depends on the nucleic acid polymerase chain reaction (PCR), the quantity and quality/purity of DNA will contribute significantly to the species authentication. In the present study, different DNA extraction and purification methods (3 classical methods and one commercial kit) were compared to produce the better isolated DNA for PCR amplification. Additionally, different methods for the estimation of DNA concentration and purity which is essential for PCR amplification efficiency were also evaluated. The result showed that classical DNA extraction methods (based on TNES-Urea) yielded a higher amount of DNA (11.30-323.60 ng/g tissue) in comparison to commercial kit/Wizard Promega (5.70-83.45 ng/g tissue). Based on the purity of DNA extract (A260/280), classical DNA extraction method produced relatively similar on DNA quality to the commercial kit (1.79-2.12). Interestingly, all classical methods produced DNA with A260/280 ratio of more than 2.00 on the blue mussel, in contrast with commercial kit. The commercial kit also produced better quality of DNA compared to the classical methods, showing the higher efficiency in PCR amplification. NanoDrop is promising as cheap, robust and safe UV-spectrophotometer method for DNA quantification, as well as the purity evaluation.Keywords: seafood authenticity, DNA isolation, polymerase chain reaction, NanoDrop, Picogreen

A novel buffer system, AnyDirect, can improve polymerase chain reaction from whole blood without DNA isolation

2007 ◽  
Vol 380 (1-2) ◽  
pp. 112-117 ◽  
Author(s):  
Young Geun Yang ◽  
Jong Yeol Kim ◽  
Young-Han Song ◽  
Doo-Sik Kim
Keyword(s):  
Polymerase Chain Reaction ◽  
Whole Blood ◽  
Dna Isolation ◽  
Buffer System ◽  
Chain Reaction ◽  
Polymerase Chain
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