Determination of Phytic Acid and Inositol Pentakisphosphates in Foods by High-Performance Ion Chromatography

2004 ◽  
Vol 52 (15) ◽  
pp. 4604-4613 ◽  
Author(s):  
Qingchuan Chen
Keyword(s):  
Phytic Acid ◽  
Ion Chromatography ◽  
High Performance

scholarly journals Comparison of High-Performance Ion Chromatography and absorptiometric methods for the determination of phytic acid in food samples

Analusis ◽  
1998 ◽  
Vol 26 (10) ◽  
pp. 396-399 ◽  
Author(s):  
P. Talamond ◽  
G. Gallon ◽  
J. P. Guyot ◽  
I. Mbome Lape ◽  
S. Trèche
Keyword(s):  
Phytic Acid ◽  
Ion Chromatography ◽  
High Performance ◽  
Food Samples

High Performance Ion Chromatography Determination of Total Sulfites in Foodstuffs

1994 ◽  
Vol 17 (2) ◽  
pp. 447-456 ◽  
Author(s):  
E. Ruiz ◽  
M. I. Santillana ◽  
M. De Alba ◽  
M. T. Neeto ◽  
S. Garcia-Castellano
Keyword(s):  
Ion Chromatography ◽  
High Performance

scholarly journals Optimization of the Treatment of Wheat Samples for the Determination of Phytic Acid by HPLC with Refractive Index Detection

2009 ◽  
Vol 92 (3) ◽  
pp. 873-878 ◽  
Author(s):  
Rosa Amaro ◽  
Miguel Murillo ◽  
Zurima Gonzlez ◽  
András Escalona ◽  
Lus Hernández
Keyword(s):  
Refractive Index ◽  
High Performance Liquid Chromatography ◽  
Phytic Acid ◽  
High Performance ◽  
Substantial Improvement ◽  
Significant Loss ◽  
Phytic Acid Content ◽  
Extraction Step ◽  
Refractive Index Detection

Abstract The treatment of wheat samples was optimized before the determination of phytic acid by high-performance liquid chromatography with refractive index detection. Drying by lyophilization and oven drying were studied; drying by lyophilization gave better results, confirming that this step is critical in preventing significant loss of analyte. In the extraction step, washing of the residue and collection of this water before retention of the phytates in the NH2 Sep-Pak cartridge were important. The retention of phytates in the NH2 Sep-Pak cartridge and elimination of the HCl did not produce significant loss (P = 0.05) in the phytic acid content of the sample. Recoveries of phytic acid averaged 91, which is a substantial improvement with respect to values reported by others using this methodology.

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