Kinetic and Stoichiometric Assessment of the Antioxidant Activity of Flavonoids by Electron Spin Resonance Spectroscopy

2003 ◽  
Vol 51 (6) ◽  
pp. 1684-1690 ◽  
Author(s):  
Donald B. McPhail ◽  
Richard C. Hartley ◽  
Peter T. Gardner ◽  
Garry G. Duthie
2012 ◽  
Vol 57 (6) ◽  
pp. 654-662 ◽  
Author(s):  
Fumihiko Yoshino ◽  
Ayaka Yoshida ◽  
Satoko Wada-Takahashi ◽  
Shuta Sugiyama ◽  
Fumiaki Tokutomi ◽  
...  

2013 ◽  
Vol 37 (6) ◽  
pp. 495-501 ◽  
Author(s):  
Jeam Haroldo Oliveira Barbosa ◽  
Jorge Antonio Gómez Luna ◽  
Angela Mitie Otta Kinoshita ◽  
Oswaldo Baffa Filho

Brazil is the largest producer of coffee in the world and coffee prices are directly linked to grain quality. In this work, the antioxidant activity of coffee was related to its quality through Electron Spin Resonance Spectroscopy (ESR), as an attempt to establish a non-subjective method to classify the grain quality. For that purpose, the IC50 and temporal monitoring of its non-oxidized fraction were determined for three bean qualities: Soft (High), Hard (Medium) and Rio (Low). Methanolic solution of 2,2-difenil-1-picril-hidrazila (DPPH), that has a stable radical and a JEOL FA-200 (X-Band) spectrometer were used for these tests. The temporal monitoring of reaction between radical and coffee was performed. The rate of reduced or of antioxidated radicals was determined on time and for each coffee beverage quality were found different slopes of curve: Soft (0.32±0.02), Hard (0.47±0.02) and Rio (0.60±0.02). The IC50 result of Rio quality (2.7 ± 0.9) was different from the Soft (7.8 ± 1.9) and Hard (6.5 ± 1.5) values, but there was no difference between the High and Medium results due to the uncertainty associated. Therefore the results found, mainly for monitoring temporal, establish a new quantitative methodology for classifying the coffee beverage quality.


2012 ◽  
Vol 47 (8) ◽  
pp. 640-643 ◽  
Author(s):  
Wanwisa Sudprasert ◽  
Sahakan Monthonwattana ◽  
Arag Vitittheeranon

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