Isolation and Characterization of Undenatured Chlorogenic Acid Free Sunflower (Helianthus annuus) Proteins

2002 ◽  
Vol 50 (6) ◽  
pp. 1713-1719 ◽  
Author(s):  
Sergio González-Pérez ◽  
Karin B. Merck ◽  
Johan M. Vereijken ◽  
Gerrit A. van Koningsveld ◽  
Harry Gruppen ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Helianthus Annuus ◽  
Isolation And Characterization

Isolation and Characterization of a Chlorogenic Acid Esterase from Aspergillus niger

1980 ◽  
Vol 35 (3-4) ◽  
pp. 209-212 ◽  
Author(s):  
B. Schöbel ◽  
W. Pollmann
Keyword(s):  
Enzyme Activity ◽  
Chlorogenic Acid ◽  
Divalent Cations ◽  
Hydrolysis Product ◽  
The Other ◽  
Temperature Optimum ◽  
Pig Liver ◽  
Isolation And Characterization

Abstract The isolation and characterization of a specific chlorogenic acid esterase is described. The enzyme activity is measured by determination of the hydrolysis product caffeic acid. The enzyme had been concentrated by means of ultrafiltration and column-chromatography. The pH- and temperature optimum were 6.5 and 45 °C respectively. Divalent cations were not required for the enzyme activity. As other esterases, this enzyme is inhibited by di-isopropyl-phosphorofluoridate. The Km-value is 0.70 mᴍ chlorogenic acid, the molecular weight 240000. The described enzyme is specific for chlorogenic acid. On the other hand a typical unspecific esterase like the pig liver esterases does not split chloro­genic acid. The isoelectric focusing reveals several isoenzymes of chlorogenase within a pI-range of 4.0-4.5.

Isolation and characterization of a mycovirus from Plasmopara halstedii

1992 ◽  
Vol 70 (9) ◽  
pp. 1734-1737 ◽  
Author(s):  
D. E. Mayhew ◽  
A. L. Cook ◽  
T. J. Gulya
Keyword(s):  
Molecular Weight ◽  
Key Words ◽  
Helianthus Annuus ◽  
Downy Mildew ◽  
Type A ◽  
Uv Absorbance ◽  
Plasmopara Halstedii ◽  
Isolation And Characterization ◽  
Virion Surface

A virus was isolated from Plasmopara halstedii, the causal agent of sunflower downy mildew. The virus contained two species of ssRNA with molecular weight (MW) of 1.05 × 106 and 0.53 × 106. One polypeptide with a MW of 37.5 × 103 was associated with whole virus. Purified preparations consisted of two components, the top component consisting primarily of empty shells. The A260/A280 UV absorbance ratio for the bottom component ranged from 1.25 to 1.35. The partially purified virions were isometric, approximately 32 nm in diameter, and had projections of approximately 4 nm on the virion surface. This virus may be related to the type A virus in Sclerophthora macrospora. Key words: mycovirus, downy mildew, Helianthus annuus.

ISOLATION AND CHARACTERIZATION OF GENES FOR THE SYNTHESIS OF CHLOROGENIC ACID IN ARTICHOKE

2012 ◽  
pp. 377-384
Author(s):  
G. Sonnante ◽  
R. D'Amore ◽  
B. Emanuela ◽  
J. Luo ◽  
D. Pignone ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Isolation And Characterization

Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid

2001 ◽  
Vol 90 (6) ◽  
pp. 873-881 ◽  
Author(s):  
D. Couteau ◽  
A.L. McCartney ◽  
G.R. Gibson ◽  
G. Williamson ◽  
C.B. Faulds
Keyword(s):  
Chlorogenic Acid ◽  
Isolation And Characterization ◽  
Colonic Bacteria

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization

Isolation and characterization of new phenanthrenes from Juncus inflexus and their chemotaxonomic significance

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
B Tóth ◽  
N Kúsz ◽  
A Csorba ◽  
T Kurtán ◽  
J Hohmann ◽  
...  
Keyword(s):  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document