Albumin proteins of eight edible grain legume species. Electrophoretic patterns and amino acid composition

1982 ◽  
Vol 30 (3) ◽  
pp. 620-622 ◽  
Author(s):  
R. S. Bhatty
1968 ◽  
Vol 46 (12) ◽  
pp. 1507-1514 ◽  
Author(s):  
J. M. Neelin ◽  
G. Vidali

Ribosomes were isolated from goose reticulocytes after lysis with saponin in 50 mM KCl, 1.5 mM MgCl2, 1 mM Tris, pH 7.5. Maximum yields of ribosomes were obtained about 4 days after injection of the birds with Phenylhydrazine, but ribosomal proteins did not vary with the stage of recovery.Ribosomal proteins (extracted with either HCl–urea or LiCl–urea) differed generally from histones (extracted with either HCl or HCl–urea) according to amino acid composition and to electrophoretic patterns in starch gel and in Polyacrylamide gel, but a few zones of ribosomal protein appeared to coincide electrophoretically with the main histone components. Since all of the former proteins were eluted unretarded from Amberlite CG-50 in 9% guanidinium chloride, in which all histones are adsorbed, we conclude that histones and ribosomal proteins are different classes of protein.The hazards of assuming chemical identities of proteins on the basis of limited electrophoretic evidence and the risks of misleading cross-contamination of cell fractions were demonstrated.


2014 ◽  
Vol 163 ◽  
pp. 97-102 ◽  
Author(s):  
Elena Pastor-Cavada ◽  
Rocío Juan ◽  
Julio E. Pastor ◽  
Manuel Alaiz ◽  
Javier Vioque

1999 ◽  
Vol 56 (3) ◽  
pp. 733-737 ◽  
Author(s):  
Angela Pierre Vitória ◽  
Ladaslav Sodek

Thirteen species of Crotalaria were analysed for nitrogen compounds in the xylem root bleeding sap. Amino acids were the main form of organic nitrogen found, but only traces of ureides were present. Of the four species analysed for amino acid composition, asparagine was found to be the major amino acid, accounting for over 68% of the nitrogen transported. No striking deviations from this general pattern was found between species, between vegetative and floral stages of development, or between nodulated and non-nodulated plants. It was concluded that the Crotalaria species studied here have an asparagine-based nitrogen metabolism, consistent with many other non-ureide-producing legume species.


2014 ◽  
Author(s):  
Alexandra Jayne Kermack ◽  
Ying Cheong ◽  
Nick Brook ◽  
Nick Macklon ◽  
Franchesca D Houghton

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