Application of the high-performance liquid chromatography-flameless atomic absorption method to the study of alkyl arsenical herbicide metabolism in soil

1982 ◽  
Vol 30 (3) ◽  
pp. 580-584 ◽  
Author(s):  
Edwin A. Woolson ◽  
Nadav Aharonson ◽  
Rosa Iadevaia
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Atomic Absorption ◽  
High Performance ◽  
Absorption Method ◽  
Flameless Atomic Absorption ◽  
Atomic Absorption Method ◽  
Herbicide Metabolism

Metabolism of Terbacil in Strawberry (Fragaria × ananassa) and Goldenrod (Solidago fistulosa)

Weed Science ◽  
1983 ◽  
Vol 31 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Andree L. Genez ◽  
Thomas J. Monaco
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gradient Elution ◽  
Solution Culture ◽  
Susceptible Cultivar ◽  
Fragaria Ananassa ◽  
Herbicide Metabolism ◽  
Minor Metabolite ◽  
Cultivar Variation

Terbacil (3-tert-butyl-5-chloro-6-methyluracil) metabolism was evaluated as a possible basis of tolerance in two species, strawberry (Fragaria × ananassaDuchesne) and goldenrod (Solidago fistulosaMiller). Reported cultivar variation in strawberry tolerance to terbacil was examined by comparing herbicide metabolism patterns in an established tolerant cultivar, 'Sunrise’, with those of the reported susceptible cultivar, ‘Guardian’. A terbacil-sensitive plant, cucumber (Cucumis sativusL. ‘Chipper’), was also used as a basis for comparison. Using gradient elution high performance liquid chromatography (HPLC), two terbacil metabolites were separated and quantitated from methanol extracts of the three species treated with14C-terbacil via roots in solution culture. The minor metabolite was identified as the non-phytotoxic derivative, 3-tert-butyl-5-chloro-6-hydroxymethyluracil, based on its co-migration with authentic 3-tert-butyl-5-chloro-6-hydroxymethyluracil in two chromatographic systems. The major metabolite was a glycoside, which yielded the hydroxylated derivative upon β-glucosidase hydrolysis. In all species, metabolites accumulated more rapidly and extensively in roots than in leaves. Metabolism was greater in the two tolerant species than in cucumber. However, the greater tolerance of goldenrod to terbacil compared to that of strawberry was apparently unrelated to differences in herbicide metabolism.

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