Rapid procedure for the determination of minute quantities of residual hydrogen peroxide in food by using a sensitive oxygen electrode

1982 ◽  
Vol 30 (2) ◽  
pp. 346-349 ◽  
Author(s):  
Masatake Toyoda ◽  
Yoshio Ito ◽  
Masahiro Iwaida ◽  
Masami Fujii
Keyword(s):  
Hydrogen Peroxide ◽  
Oxygen Electrode ◽  
Rapid Procedure ◽  
Residual Hydrogen

scholarly journals Improved Protocol for an Oxygen Electrode Method for Determining Hydrogen Peroxide in Foods

1997 ◽  
Vol 80 (3) ◽  
pp. 681-687 ◽  
Author(s):  
Fumio Miyamoto ◽  
Masanobu Saeki ◽  
Takumi Yoshizawa
Keyword(s):  
Hydrogen Peroxide ◽  
Standard Method ◽  
Present Method ◽  
Accurate Determination ◽  
Oxygen Electrode ◽  
Nitrogen Gas ◽  
Naturally Occurring ◽  
Residual Hydrogen ◽  
Electrode Method

Abstract An oxygen electrode method for determining residual hydrogen peroxide in foods has been further improved. Pretreatment, which includes extraction and neutralization, is done in a hydrogen peroxide extraction apparatus with nitrogen gas bubbling. The hydrogen peroxide concentration of the sample is corrected by subtracting the sample blank value, obtained for the sample through catalase treatment. Bubbling with nitrogen gas effectively minimized the sample blank value, making this method suitable for accurate determination of trace amounts of hydrogen peroxide in foods. Recoveries of hydrogen peroxide added at 1-10 μg/g were 77.8-107.1% by the present method. These recoveries are similar to or higher than those by the Japanese standard method and by another modified oxygen electrode method. Concentrations of naturally occurring hydrogen peroxide in solid foods were <0.87 μg/g by the present method, lower than those by either the standard method or another modified oxygen electrode method.

A biospecific membrane sensor for the determination of sucrose

1983 ◽  
Vol 48 (3) ◽  
pp. 798-804 ◽  
Author(s):  
Lumír Macholán ◽  
Hana Konečná
Keyword(s):  
Thin Film ◽  
Hydrogen Peroxide ◽  
Steady State ◽  
Reaction Layer ◽  
Enzyme Reaction ◽  
Oxygen Electrode ◽  
Reaction Vessel ◽  
Membrane Sensor ◽  
Electrode Response

The paper describes a rapid method for the biospecific determination of sucrose using an oxygen electrode of the Clark type or a platinum disc anode, the measuring part of which is coated by a thin film of invertase, mutarotase and glucose oxidase co-crosslinked by glutardialdehyde together with serum albumin. After injecting the sample into the reaction vessel the current corresponding to the decrease of the oxygen content or to the formation of hydrogen peroxide in the enzyme reaction layer is registered. The steady state electrode response is proportional to the concentration of sucrose within the range of 0.03 to 1.5 mmol . l-1 and is attained during 1-2 minutes with a reproducibility of 3-4%.

AN IMPROVED METHOD FOR THE DETERMINATION OF RESIDUAL HYDROGEN PEROXIDE IN MILK

1977 ◽  
Vol 42 (2) ◽  
pp. 537-538 ◽  
Author(s):  
C. B. GUPTA ◽  
N. A. M. ESKIN ◽  
C. FRENKEL ◽  
A. Y. SADOVSKI
Keyword(s):  
Hydrogen Peroxide ◽  
Improved Method ◽  
Residual Hydrogen

scholarly journals Thermal stability for the effective use of commercial catalase

2014 ◽  
Vol 16 (4) ◽  
pp. 75-79 ◽  
Author(s):  
J. Miłek ◽  
M. Wójcik ◽  
W. Verschelde
Keyword(s):  
Thermal Stability ◽  
Hydrogen Peroxide ◽  
Half Life ◽  
Textile Industry ◽  
Oxygen Electrode ◽  
Thermal Deactivation ◽  
Residual Hydrogen ◽  
Effective Use ◽  
First Time ◽  
Kinetics Of

Abstract Catalase with the commercial catalase name Terminox Ultra is widely used in the textile industry in bleaching processes. This enzyme is used to catalyse the decomposition of residual hydrogen peroxide into oxygen and water. In this study catalase was kept for about 30 hours in water baths in a temperature range from 35 to 70°C. For the first time, the kinetics of thermal deactivation of this enzyme was examined using an oxygen electrode. Stability of the enzyme depends strongly on temperature and its half-life times are 0.0014 h and 7.6 h, at 35 and 70°C, respectively.

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