Determination of aspartic acid, phenylalanine, and aspartylphenylalanine in aspartame-containing samples using a precolumn derivatization HPLC method

1990 ◽  
Vol 38 (5) ◽  
pp. 1256-1260 ◽  
Author(s):  
Kazuichi Hayakawa ◽  
Tanya Schilpp ◽  
Kazuhiro Imai ◽  
Takeru Higuchi ◽  
Osborne S. Wong
Keyword(s):  
Aspartic Acid ◽  
Hplc Method ◽  
Precolumn Derivatization

A modified HPLC method for the determination of aspartic acid racemization in collagen from human dentin and its comparison with GC

2004 ◽  
Vol 27 (4) ◽  
pp. 330-334 ◽  
Author(s):  
Terezie Benešová ◽  
Aleš Honzátko ◽  
Alexandr Pilin ◽  
Jaroslav Votruba ◽  
Miroslav Flieger
Keyword(s):  
Aspartic Acid ◽  
Hplc Method ◽  
Aspartic Acid Racemization ◽  
Human Dentin

scholarly journals Determination of Amino Acids of Cultivated Species of the Genus Primula L.

2020 ◽  
Vol 11 (2) ◽  
pp. 8969-8977
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Hplc Method ◽  
Raw Material ◽  
Cultivated Species ◽  
Medicinal Properties ◽  
The Rich ◽  
High Concentrations

Throughout many years, plants using not only as a source of the meal but also in the fight against diseases. Among these plants are rock primrose (Primula saxatilis Коm.), Julia’s primrose (Primula juliae Kusn.), and drumstick primrose (Primula denticulatа Smith.). The aim of our research was to determine the content of amino acids in these plants with the future prospects of their application as officinal medicinal plant raw material. The amino acids of the leaves of cultivated species of the genus Primula L. determined by the HPLC method. 16 free and 16 bound amino acids were identified in the cultivated species of Primula L. High concentrations of the free amino acids that as L-glutamic acid, L-aspartic acid, and L-alanine predominate in all the plants analyzed. Among the bound amino acids in the leaves of cultivated Primula species prevail, L-glutamic acid, L-aspartic acid, L-lysine, and L-leucine. The results show that rock primrose, Julia’s primrose, and drumstick primrose are the rich source of amino acids. The specific metabolic processes in which these amino acids are involved may be related to the medicinal properties of plants according to their use in traditional medicine, and hence may contribute to the understanding of their healing properties.

Plasma amino acids determined by liquid chromatography within 17 minutes

1994 ◽  
Vol 40 (2) ◽  
pp. 245-249 ◽  
Author(s):  
T Terrlink ◽  
P A van Leeuwen ◽  
A Houdijk
Keyword(s):  
Amino Acids ◽  
Reversed Phase ◽  
Hplc Method ◽  
Complete Separation ◽  
Analysis Time ◽  
Precolumn Derivatization ◽  
Short Analysis Time ◽  
Primary Amino ◽  
Total Analysis

Abstract We present an HPLC method for the determination of amino acids in plasma. The method is based on automated precolumn derivatization of amino acids with o-phthalaldehyde, separation of the derivatives by reversed-phase chromatography, and quantification by fluorescence detection. Complete separation was achieved within 12 min. Total analysis time, including derivatization, chromatography, and reequilibration of the column, was 17 min. The assay was linear from 5 to 800 mumol/L for all amino acids. Recovery of amino acids added to plasma samples was 96-106%, except for tryptophan (89%). Within-run precision (CV) was 1.8-6.4%, and between-run precision was 2.1-7.2%. The method can be used for determining primary amino acids in plasma and cerebrospinal fluid. The simple sample preparation and short analysis time make the method particularly suitable for routine analysis of large series of samples.

scholarly journals Determination of total aminothiols and neuroactive amino acids in plasma by high performance liquid chromatography with fluorescence detection

2010 ◽  
Vol 56 (6) ◽  
pp. 726-738 ◽  
Author(s):  
V.K. Pozdeev ◽  
N.V. Pozdeyev
Keyword(s):  
Amino Acids ◽  
Disulfide Bonds ◽  
High Performance ◽  
Signal To Noise Ratio ◽  
Iodoacetic Acid ◽  
Reversed Phase ◽  
Hplc Method ◽  
Precolumn Derivatization ◽  
Neuroactive Amino Acids

This paper describes a simple and sensitive reversed-phase HPLC method for the determination of total homocysteine, total cysteine, total glutathione (GSH+GSSG), and neuroactive amino acids (Asp, Glu, Tau, GABA) using precolumn derivatization with ortho-phtaldialdehyde and fluorimetric detection at 360 and 470 nm for emission and excitation, respectively. Derivatization was performed with ortho-phthaldialdehyde in the presence of 2-mercaptoethanol after alkylation of the free sulfhydryl groups with iodoacetic acid. For determination of total aminothiols, the disulfide bonds were reduced and protein-bound thiols were released by addition of dithiothreitol to the plasma sample. The advantage of this method is the simultaneous determination of both homocysteine/cysteine/glutathione and neuroactive amino acids in the sample. The plasma levels of studied compounds were determined in 14 healthy volunteers (20-45 years old) and 55 patients with chronic hepatitis C (20-49 years old) and the resulting numbers were in a good agreement the studies published earlier. The calibration curves were linear over a concentration range of 5-100 μM in plasma (r2=0.985-0.996). The intraday and interday coefficients of variation were 3-6% and 4-7%, respectively. The recovery of the standards added to the plasma samples ranged from 94 to 102%. The limits of detection (LOD) were 0.2-0.5 ng per 10 μl injection volume (signal-to-noise ratio of 3).

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