Purification and characterization of neomycin phosphotransferase II from genetically modified cottonseed (Gossypium hirsutum)

1995 ◽  
Vol 43 (4) ◽  
pp. 1105-1109 ◽  
Author(s):  
David C. Wood ◽  
Linh V. Vu ◽  
Nancee M. Kimack ◽  
Glennon J. Rogan ◽  
Joel E. Ream ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Genetically Modified ◽  
Neomycin Phosphotransferase ◽  
Purification And Characterization ◽  
Neomycin Phosphotransferase Ii

Purification and Characterization of Microbially Expressed Neomycin Phosphotransferase II (NPTII) Protein and its Equivalence to the Plant Expressed Protein

1993 ◽  
Vol 11 (12) ◽  
pp. 1537-1542 ◽  
Author(s):  
Roy L. Fuchs ◽  
Robert A. Heeren ◽  
Mark E. Gustafson ◽  
Glen J. Rogan ◽  
David E. Bartnicki ◽  
...  
Keyword(s):  
Neomycin Phosphotransferase ◽  
Purification And Characterization ◽  
Neomycin Phosphotransferase Ii

Purification and Characterization of Cell Suspensions Peroxidase from Cotton (Gossypium hirsutum L.)

2008 ◽  
Vol 157 (3) ◽  
pp. 575-592 ◽  
Author(s):  
Tanoh Hilaire Kouakou ◽  
Edmond Ahipo Dué ◽  
N’guessan Eugène Jean Parfait Kouadio ◽  
Sébastien Niamké ◽  
Yatty Justin Kouadio ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cell Suspensions ◽  
Purification And Characterization ◽  
Gossypium Hirsutum L

Promoter isolation and characterization of GhAO-like1, a Gossypium hirsutum gene similar to multicopper oxidases that is highly expressed in reproductive organs

Genome ◽  
2016 ◽  
Vol 59 (1) ◽  
pp. 23-36 ◽  
Author(s):  
Julia Lambret-Frotté ◽  
Sinara Artico ◽  
Sarah Muniz Nardeli ◽  
Fernando Fonseca ◽  
Osmundo Brilhante Oliveira-Neto ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Promoter Region ◽  
Genetically Modified ◽  
Ascorbate Oxidase ◽  
Specific Expression ◽  
Regulatory Regions ◽  
Tissue Specific ◽  
Isolation And Characterization

Cotton is one of the most economically important cultivated crops. It is the major source of natural fiber for the textile industry and an important target for genetic modification for both biotic stress and herbicide tolerance. Therefore, the characterization of genes and regulatory regions that might be useful for genetic transformation is indispensable. The isolation and characterization of new regulatory regions is of great importance to drive transgene expression in genetically modified crops. One of the major drawbacks in cotton production is pest damage; therefore, the most promising, cost-effective, and sustainable method for pest control is the development of genetically resistant cotton lines. Considering this scenario, our group isolated and characterized the promoter region of a MCO (multicopper oxidase) from Gossypium hirsutum, named GhAO-like1 (ascorbate oxidase-like1). The quantitative expression, together with the in vivo characterization of the promoter region reveals that GhAO-like1 has a flower- and fruit-specific expression pattern. The GUS activity is mainly observed in stamens, as expected considering that the GhAO-like1 regulatory sequence is enriched in cis elements, which have been characterized as a target of reproductive tissue specific transcription factors. Both histological and quantitative analyses in Arabidopsis thaliana have confirmed flower (mainly in stamens) and fruit expression of GhAO-like1. In the present paper, we isolated and characterized both in silico and in vivo the promoter region of the GhAO-like1 gene. The regulatory region of GhAO-like1 might be useful to confer tissue-specific expression in genetically modified plants.

EVALUATION OF PROCEDURES FOR THE EXTRACTION AND PURIFICATION OF NEOMYCIN PHOSPHOTRANSFERASE II FROM A GENETICALLY MODIFIED AGROBACTERIUM

2004 ◽  
Vol 94 (12) ◽  
pp. 93-99
Author(s):  
Laura Anfossi ◽  
Cinzia Tozzi ◽  
Gianfranco Giraudi ◽  
Cristina Giovannoli ◽  
Claudio Baggiani ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Neomycin Phosphotransferase ◽  
Neomycin Phosphotransferase Ii ◽  
Extraction And Purification
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