Purification and Properties of Aminopeptidase H from Bovine Skeletal Muscle

1994 ◽  
Vol 42 (12) ◽  
pp. 2709-2712 ◽  
Author(s):  
Toshihide Nishimura ◽  
Mee R. Rhyu ◽  
Hiromichi Kato ◽  
Soichi Arai
Keyword(s):  
Skeletal Muscle ◽  
Purification And Properties ◽  
Bovine Skeletal Muscle

scholarly journals Purification and Properties of Bovine Skeletal Muscle Proteasome

2005 ◽  
Vol 18 (6) ◽  
pp. 884-891
Author(s):  
S. Yamamoto ◽  
B. Gerelt ◽  
T. Nishiumi ◽  
A. Suzuki
Keyword(s):  
Skeletal Muscle ◽  
Purification And Properties ◽  
Bovine Skeletal Muscle

Mammalian skeletal muscle C-protein: purification from bovine muscle, binding to titin and the characterization of a full-length human cDNA

1992 ◽  
Vol 102 (4) ◽  
pp. 769-778
Author(s):  
D.O. Furst ◽  
U. Vinkemeier ◽  
K. Weber
Keyword(s):  
Skeletal Muscle ◽  
Protein Sequencing ◽  
Protein Isoforms ◽  
Fast Method ◽  
Mammalian Skeletal Muscle ◽  
Terminal Sequence ◽  
C Protein ◽  
Human Sequence ◽  
Associated Proteins ◽  
Bovine Skeletal Muscle

We report a fast method for the isolation of homogeneous C-protein from bovine skeletal muscle. In electron micrographs C-protein appears as short rods with a relatively uniform length of about 50 nm. Protein sequencing shows a single N-terminal sequence. Radio-labelled C-protein strongly decorates titin II and myosin rods but not myosin heads. Binding to titin II is retained in preparations lacking titin-associated proteins. Antibodies to bovine C-protein were used to screen a lambda gt11 cDNA library constructed from fetal human skeletal muscle. Clone HC38 is 3833 bp long and encodes a protein of 1138 amino acid residues. The start of the predicted sequence fits the N-terminal sequence of the bovine protein. All partial sequences obtained from the bovine protein (348 residues) and the sequence deduced from a partial chicken cDNA (Einheber and Fischman, 1990) can be aligned along the human sequence. The sequences of human and chicken C-proteins share 50% identity and 70% similarity. Along the repeat patterns of the human protein the fibronectin (Fn)-like domains are better conserved than the immunoglobulin (Ig)-like domains. Regions of strong divergence between chicken fast C-protein and human slow C-protein may represent differences in C-protein isoforms.

Sign in / Sign up

Export Citation Format

Share Document