Development of a quantitative and sensitive enzyme-linked immunosorbent assay for ochratoxin A using antibodies from the yolk of the laying hen

1993 ◽  
Vol 41 (10) ◽  
pp. 1784-1789 ◽  
Author(s):  
J. R. Clarke ◽  
R. R. Marquardt ◽  
A. Oosterveld ◽  
A. A. Frohlich ◽  
F. J. Madrid ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Laying Hen ◽  
Enzyme Linked Immunosorbent Assay

MYCOTOXINS IN BEANS OF PEANUTS GROWN IN TAJIKISTAN

2018 ◽  
Vol 1 (4) ◽  
pp. 74-77
Author(s):  
Sh. I. Razokov ◽  
◽  
D. M. Mirzoev ◽  
G. P. Kononenko ◽  
A. A. Burkin ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Cyclopiazonic Acid ◽  
Practical Significance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Systems ◽  
The Republic ◽  
Combined Contamination

The article presents the results of an extensive mycotoxicological examination of 11 samples of peanut beans grown in two regions of the Republic of Tajikistan. The determination of 16 mycotoxins was carried out by indirect competitive enzyme-linked immunosorbent assay using commercial and certified research test systems. It has been established that for peanut beans in this area, a combined contamination by a group of sanitary-significant mycotoxins, including diacetoxyscirpenol, alternariol, ochratoxin A, PR-toxin and cyclopiazonic acid, is characteristic. The prospects of further research and the practical significance of the results are discussed.

scholarly journals Mycotoxins as a risk in the grain food

2009 ◽  
pp. 79-86 ◽  
Author(s):  
Jovana Matic ◽  
Jasna Mastilovic ◽  
Ivana Cabarkapa ◽  
Anamarija Mandic
Keyword(s):  
European Union ◽  
Secondary Metabolites ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Toxic Effects ◽  
Enzyme Linked Immunosorbent Assay ◽  
The European Union ◽  
The Mean ◽  
Grain Foods

Mycotoxins are toxic secondary metabolites of fungi that contaminate a large variety of foods and have toxic effects on humans. The best protection against mycotoxins is to monitor their presence in food. This paper shows the screening results of mycotoxins present in 76 samples of different groups of grain foods. Samples of grain food were analyzed for contamination with aflatoxins, ochratoxin A, zearalenone, fumonisins and deoxynivalenol. Analysis were conducted using competitive enzyme-linked immunosorbent assay (ELISA). None of the samples was contaminated with aflatoxins. The most predominant mycotoxin was ochratoxin A with the mean level of 4.84 ? 4.49 ppb in 19.7% of the examined samples. Zearalenone, fumonisins, and deoxynivalenol were found in 9.21, 14.5 and 3.9% of the samples, respectively. Mycotoxin content in the investigated samples was compared with the regulations of Serbia and those of the European Union.

scholarly journals Aflatoxigenic and ochratoxigenic moulds and their toxins in tree nuts on Serbian market

2020 ◽  
pp. 9-18
Author(s):  
Irena Rakic ◽  
Gordana Dimic ◽  
Marija Skrinjar ◽  
Suncica Kocic-Tanackov
Keyword(s):  
Aspergillus Niger ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Alternaria Alternata ◽  
Rhizopus Oryzae ◽  
Limit Of Detection ◽  
Average Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mean Values ◽  
Tree Nuts

In this study, moulds and mycotoxins presence in different tree nuts were investigated. The results showed that all of the 25 samples were contaminated with moulds. Mean values of total mould count varied from 1-4.9 cfu per grain. The most frequent species in hazelnut samples were Rhizopus oryzae (32.2%) and Aspergillus niger (28.9%). In walnuts A. niger (75.6%), in cashews also A. niger (42.4%) while in pistachio samples Alternaria alternata (20.7%), and Cladosporium cladosporioides (20.7%) were the most dominant. Rhizopus oligosporus was the only identified species in all almond samples (100%). Using Enzyme Linked Immunosorbent Assay (ELISA), the presence of total aflatoxins and ochratoxin A was examinated. In all analyzed samples, levels of ochratoxin A were below the limit of detection. Total aflatoxins were detected only in walnut samples with average concentration of 7.1 ?g/kg.

Development of a Sensitive Enzyme-Linked Immunosorbent Assay for the Determination of Ochratoxin A

2005 ◽  
Vol 53 (17) ◽  
pp. 6947-6953 ◽  
Author(s):  
Feng-yih Yu ◽  
Tsuen-feng Chi ◽  
Biing-hui Liu ◽  
Ching-chyuan Su
Keyword(s):  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

The Development of a Quantitative ELISA for Aflatoxin B1 in Ground Peanuts Using Antibodies Isolated From the Yolk of a Laying Hen

1994 ◽  
Vol 57 (11) ◽  
pp. 1022-1024 ◽  
Author(s):  
SUZHEN LI ◽  
RONALD R. MARQUARDT ◽  
ANDREW A. FROHLICH ◽  
HAO XIAO ◽  
JAMES R. CLARKE
Keyword(s):  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Laying Hens ◽  
Egg Yolk ◽  
Laying Hen ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coating Antigen ◽  
Extraction Protocol

Antibodies directed against Aflatoxin B1 (AFB1) were produced in laying hens, isolated from egg yolk and applied in an enzyme-linked immunosorbent assay (ELISA) for AFB1 in ground peanuts. The ELISA sensitivity was improved by reduction of the amount of the coating antigen absorbed onto the microplate well surfaces. The main aflatoxins B1, B2, G1, G2, M1, aflatoxicol, sterigmatocystin were found to cross-react in the competitive ELISA, 100, 25, 23, 4, 0, 0, 0%, respectively. Aflatoxin B1 could be reproducibly detected and quantified in spiked ground peanuts at levels greater than 5 ppb using a hexane and methanol solvent based peanut extraction protocol.

scholarly journals Production of monoclonal antibodies reactive with ochratoxin A: Enzyme-linked immunosorbent assay for detection of ochratoxin A

1985 ◽  
Vol 1985 (21) ◽  
pp. 28-29 ◽  
Author(s):  
J. CHIBA ◽  
H. KAJII ◽  
M. KAWAMURA ◽  
K. OHI ◽  
N. MOROOKA ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

A sensitive enzyme-linked immunosorbent assay of ochratoxin A based on monoclonal antibodies

Toxicon ◽  
1989 ◽  
Vol 27 (8) ◽  
pp. 887-897 ◽  
Author(s):  
O. Kawamura ◽  
S. Sato ◽  
H. Kajii ◽  
S. Nagayama ◽  
K. Ohtani ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Mycotoxins in maize: Annual variations and the impact of climate change

2017 ◽  
pp. 63-70 ◽  
Author(s):  
Jovana Kos ◽  
Elizabet Janic-Hajnal ◽  
Anamarija Mandic ◽  
Olivera Djuragic ◽  
Pavle Jovanov ◽  
...  
Keyword(s):  
Climate Change ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Weather Conditions ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Annual Variations ◽  
Growing Seasons ◽  
The Republic ◽  
The Impact

The presence of aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), and fumonisins (FUMs) was examined in maize samples from the Republic of Serbia. The maize samples were collected during the period 2012-2016, and analyzed every year after harvest using validated Enzyme Linked Immunosorbent Assay (ELISA) method. The obtained results were considered regarding the weather conditions parameters recorded during the investigated maize growing seasons. Significant differences in weather-related parameters recorded in the five-year period resulted in different mycotoxin profiles between the investigated years. Obtained results indicate that the presence of ZEA and DON in maize is characteristic of years with abundant precipitation, while AFs and OTA mainly occur in maize during hot and dry years. Furthermore, FUMs were detected with different contamination frequency in maize samples from every year. Based on the findings obtained in this study, as well as on noted changes in weather conditions in the recent years it could be assumed that maize from Serbia may become susceptible to problems concerning mycotoxins. Therefore, there is a necessity for monitoring and research related to the mycotoxins occurrence in maize from Serbia.

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