Partial purification of soluble potato polyphenol oxidase by partitioning in an aqueous two-phase system

1993 ◽  
Vol 41 (8) ◽  
pp. 1219-1224 ◽  
Author(s):  
Alvaro. Sanchez-Ferrer ◽  
Francisco. Laveda ◽  
Francisco. Garcia-Carmona
Keyword(s):  
Polyphenol Oxidase ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Partial Purification of Polyphenol Oxidase (PPO) from Mango Seed Kernel (MSK) using Alcohol/Salt-based Aqueous Two-Phase System (ATPS)

2021 ◽  
Vol 10 ◽  
Author(s):  
John Leanard M. Lasquite ◽  
Brendaline C. Enopia ◽  
Roger Francis E. Senangote ◽  
Danilo C. Sabarre Jr. ◽  
Camila Flor Yagonia-Lobarbio
Keyword(s):  
Polyphenol Oxidase ◽  
Alternative Methods ◽  
Phase System ◽  
Seed Kernel ◽  
Mass Ratio ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Activity Recovery ◽  
Mango Seed Kernel ◽  
Mango Seed

Background: Polyphenol oxidase (PPO) has applications in pharmaceutical, environmental, and food industries, however, conventional techniques for its purification are considered costly and laborious. Alternative methods of purification are needed. Methods: This study explored the use of an alcohol/salt-based aqueous two-phase system (ATPS) to partially purify PPO from mango seed kernel (MSK). Specifically, this study determined the effects of 1-propanol/potassium phosphate buffer mass ratio on the partition parameters such as partition coefficients, activity recovery, and purification fold. Results: The enzyme partition coefficient (K E ) was greater than 1, which signified that more PPO was partitioned in the top phase due to its hydrophobic nature. Specifically, the 3.22 1-propanol/PPB mass ratio had the highest K E of 1.91 ± 0.16 and 1.46 ± 0.16 for runs 1 and 2, respectively. Moreover, the same mass ratio led to the highest activity recovery in the top phase reaching up to 73.99 ± 1.56% for run 1 and 64.27 ± 2.48% for run 2. In terms of purification fold, runs 1 and 2 had PF values of 0.55 ± 0.05 and 0.70 ± 0.01, respectively. Since the values obtained were low due to the large number of contaminants extracted together with the target enzyme in the top phase, the use of a back-extraction step recovered the PPO in the bottom phase and resulted in an activity recovery of 82.94 ± 1.05% and a purification fold of 7.01 ± 0.95. Conclusion: Results have shown that varying the mass ratio had significant effects on the partition parameters.

Purification of potato polyphenol oxidase (PPO) by partitioning in aqueous two-phase system

2006 ◽  
Vol 28 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Bhalchandra K. Vaidya ◽  
Hitesh K. Suthar ◽  
Sangita Kasture ◽  
Sanjay Nene
Keyword(s):  
Polyphenol Oxidase ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals A novel and environmentally friendly bioprocess for separation and partial purification of polysaccharides from Cordyceps sinensis mycelia by an aqueous two-phase system

RSC Advances ◽  
2017 ◽  
Vol 7 (60) ◽  
pp. 37659-37665 ◽  
Author(s):  
Zhigang Li ◽  
Ailin Chen ◽  
Zhencheng Li ◽  
Man Qu ◽  
Huayong Chen ◽  
...  
Keyword(s):  
High Efficiency ◽  
Environmentally Friendly ◽  
Cordyceps Sinensis ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P ◽  
Active Substances

An aqueous two-phase system comprising ethanol and salt not only could purify CS polysaccharides with high efficiency, but also simultaneously separated multiple active substances through coupling with ultrafiltration.

Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system

2017 ◽  
Vol 52 (5) ◽  
pp. 834-842 ◽  
Author(s):  
Hadi Sofijan ◽  
Tan Joo Shun ◽  
Sahar Abbasiliasi ◽  
Shuhaimi Mustafa ◽  
Nyoman Tri Puspaningsih ◽  
...  
Keyword(s):  
Bacillus Megaterium ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Recovery and partial purification of fibrinolytic enzymes of Auricularia polytricha (Mont.) Sacc by an aqueous two-phase system

2014 ◽  
Vol 122 ◽  
pp. 359-366 ◽  
Author(s):  
Sharjahan Mohamed Ali ◽  
Tau Chuan Ling ◽  
Sekaran Muniandy ◽  
Yee Shin Tan ◽  
Jegadeesh Raman ◽  
...  
Keyword(s):  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Fibrinolytic Enzymes ◽  
Auricularia Polytricha ◽  
Aqueous Two Phase System

Extraction of protease from ora‐pro‐nobis ( Pereskia aculeata Miller) and partial purification in polyethylene glycol + sodium phosphate aqueous two‐phase system

2021 ◽  
Author(s):  
Annie Nolasco Alves ◽  
Priscilla Amaral Nascimento ◽  
Rafael da Costa Ilhéu Fontan ◽  
Evaldo Cardozo de Sousa Júnior ◽  
Paulo Bonomo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Sodium Phosphate ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Partial purification of fibrinolytic and fibrinogenolytic protease from Gliricidia sepium seeds by aqueous two-phase system

2020 ◽  
Vol 27 ◽  
pp. 101669 ◽  
Author(s):  
Aníbia Vicente da Silva ◽  
Jéssica Miranda do Nascimento ◽  
Cláudio Henrique Rodrigues ◽  
Débora Cristina Silva Nascimento ◽  
Romero Marcos Pedrosa Brandão Costa ◽  
...  
Keyword(s):  
Gliricidia Sepium ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

2021 ◽  
Author(s):  
Qiaoshu Chen ◽  
Yanwen Zhang ◽  
Hui Chen ◽  
Jianbo Liu ◽  
Juewen Liu
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System
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