Liquid−Liquid Equilibrium of the Aqueous Two-Phase System Water + PEG 4000 + Potassium Phosphate at Four Temperatures:  Experimental Determination and Thermodynamic Modeling

2002 ◽  
Vol 47 (6) ◽  
pp. 1401-1405 ◽  
Author(s):  
Rogério A. G. Sé ◽  
Martín Aznar
Keyword(s):  
Experimental Determination ◽  
Thermodynamic Modeling ◽  
Potassium Phosphate ◽  
Phase System ◽  
Liquid Equilibrium ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Peg 4000 ◽  
System Water

scholarly journals Purification of Bromelain Enzyme from Curauá (Ananaserectifolius LB Smith) White Variety, by Aqueous Two-Phase System PEG 4000/Potassium Phosphate

2014 ◽  
Vol 8 (4) ◽  
Author(s):  
Juliana Ferrari Ferreira ◽  
Dalva Sbruzzi ◽  
Kleber Vânio Gomes Barros ◽  
Daniela Diniz Ehrhardt ◽  
Elias Basile Tambourgi
Keyword(s):  
Potassium Phosphate ◽  
Phase System ◽  
Two Phase ◽  
White Variety ◽  
Aqueous Two Phase System ◽  
Peg 4000

Liquid–Liquid Equilibrium Data and Thermodynamic Modeling for Aqueous Two-Phase System Peg 1500 + Sodium Sulfate + Water at Different Temperatures

2019 ◽  
Vol 64 (2) ◽  
pp. 810-816 ◽  
Author(s):  
Cláudia Lais Rodrigues Barreto ◽  
Sérgio de Sousa Castro ◽  
Evaldo Cardozo de Souza Júnior ◽  
Cristiane Martins Veloso ◽  
Lizzy Ayra Alcântara Veríssimo ◽  
...  
Keyword(s):  
Thermodynamic Modeling ◽  
Sodium Sulfate ◽  
Phase System ◽  
Liquid Equilibrium ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Equilibrium Data ◽  
Different Temperatures

scholarly journals Optimization of Aqueous Two-Phase System (ATPS) of Recombinant Bromelain by Response Surface Methodology

2018 ◽  
Vol 7 (4.30) ◽  
pp. 377
Author(s):  
Zatul Iffah Mohd Arshad ◽  
Azura Amid
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Partition Coefficient ◽  
Response Surface ◽  
Potassium Phosphate ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Crude Sample ◽  
Face Centered

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.

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