scholarly journals Top-Down Analysis of In-Source HDX of Native Protein Ions

2020 ◽  
Vol 31 (5) ◽  
pp. 1151-1154
Author(s):  
Nawaporn Sanguantrakun ◽  
Chamnongsak Chanthamontri ◽  
Michael L. Gross
Keyword(s):  
Native Protein ◽  
Top Down ◽  
Protein Ions

scholarly journals Top-Down Characterization of Denatured Proteins and Native Protein Complexes Using Electron Capture Dissociation Implemented within a Modified Ion Mobility-Mass Spectrometer

2020 ◽  
Vol 92 (5) ◽  
pp. 3674-3681 ◽  
Author(s):  
Jonathan P. Williams ◽  
Lindsay J. Morrison ◽  
Jeffery M. Brown ◽  
Joseph S. Beckman ◽  
Valery G. Voinov ◽  
...  
Keyword(s):  
Electron Capture ◽  
Mass Spectrometer ◽  
Electron Capture Dissociation ◽  
Ion Mobility ◽  
Protein Complexes ◽  
Native Protein ◽  
Top Down ◽  
Denatured Proteins

Pervasive Charge Solvation Permeates Native-like Protein Ions and Dramatically Influences Top-down Sequencing Data

2020 ◽  
Vol 142 (14) ◽  
pp. 6750-6760
Author(s):  
Daniel A. Polasky ◽  
Sugyan M. Dixit ◽  
Michael F. Keating ◽  
Varun V. Gadkari ◽  
Philip C. Andrews ◽  
...  
Keyword(s):  
Sequencing Data ◽  
Top Down ◽  
Protein Ions ◽  
Charge Solvation

scholarly journals Proteoform-selective imaging of tissues using mass spectrometry

2021 ◽  
Author(s):  
Manxi Yang ◽  
Hang Hu ◽  
Pei Su ◽  
Paul M. Thomas ◽  
Jeannie M. Camarillo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Biological Tissues ◽  
Ionization Mass ◽  
Top Down ◽  
Tissue Sections ◽  
Spatially Resolved ◽  
Multiply Charged ◽  
Specific Analysis ◽  
Protein Ions

Unraveling the complexity of biological systems relies on the development of new approaches for spatially resolved proteoform-specific analysis of the proteome. Top-down proteomics is a powerful tool, which has been used for the identification of thousands of proteoforms in biological samples. Herein, we present a first spatially resolved top-down proteomics analysis of biological tissues using nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI). Nano-DESI generates multiply charged protein ions, which is advantageous for their structural characterization using tandem mass spectrometry (MS/MS). Proof-of-concept experiments demonstrate that the nano-DESI MSI combined with on-tissue top-down proteomics is ideally suited for the proteoform-selective imaging of thin tissue sections. Using rat brain tissue as a model system, we provide the first evidence of the differential proteoform expression in different regions of the brain.

Top-Down Processing and Visual Reorientation Illusions in a Virtual Reality Environment

2004 ◽  
Vol 63 (3) ◽  
pp. 143-149 ◽  
Author(s):  
Fred W. Mast ◽  
Charles M. Oman
Keyword(s):  
Virtual Reality ◽  
Visual Processing ◽  
Line Length ◽  
Perceptual Cues ◽  
Virtual Reality Environment ◽  
Top Down ◽  
Test Conditions ◽  
The Subject ◽  
Processing Mechanisms

The role of top-down processing on the horizontal-vertical line length illusion was examined by means of an ambiguous room with dual visual verticals. In one of the test conditions, the subjects were cued to one of the two verticals and were instructed to cognitively reassign the apparent vertical to the cued orientation. When they have mentally adjusted their perception, two lines in a plus sign configuration appeared and the subjects had to evaluate which line was longer. The results showed that the line length appeared longer when it was aligned with the direction of the vertical currently perceived by the subject. This study provides a demonstration that top-down processing influences lower level visual processing mechanisms. In another test condition, the subjects had all perceptual cues available and the influence was even stronger.

Functional Anatomy of Intensity Aspects of Attention

2003 ◽  
Vol 14 (3) ◽  
pp. 181-190 ◽  
Author(s):  
Walter Sturm
Keyword(s):  
Right Hemisphere ◽  
Functional Anatomy ◽  
Fmri Data ◽  
Functional Networks ◽  
Top Down ◽  
Stimulus Modality ◽  
Spatial Orienting ◽  
Phasic Alertness ◽  
Co Activation ◽  
Attention System

Abstract: Behavioral and PET/fMRI-data are presented to delineate the functional networks subserving alertness, sustained attention, and vigilance as different aspects of attention intensity. The data suggest that a mostly right-hemisphere frontal, parietal, thalamic, and brainstem network plays an important role in the regulation of attention intensity, irrespective of stimulus modality. Under conditions of phasic alertness there is less right frontal activation reflecting a diminished need for top-down regulation with phasic extrinsic stimulation. Furthermore, a high overlap between the functional networks for alerting and spatial orienting of attention is demonstrated. These findings support the hypothesis of a co-activation of the posterior attention system involved in spatial orienting by the anterior alerting network. Possible implications of these findings for the therapy of neglect are proposed.

Auswirkungen eines Aufmerksamkeitstrainings auf die aphasische Symptomatik bei Schlaganfallpatienten

2011 ◽  
Vol 22 (1) ◽  
pp. 21-32 ◽  
Author(s):  
Julia Graf ◽  
Hartwig Kulke ◽  
Christa Sous-Kulke ◽  
Wilfried Schupp ◽  
Stefan Lautenbacher
Keyword(s):  
Top Down

Aufmerksamkeit kann als Kontrollsystem neuronaler Aktivität verstanden werden, welches Neuroplastizität top-down modulieren hilft. Bisher wurde selten versucht, durch deren gezielte Förderung Funktionswiederherstellungen nach Hirnschädigung zu begünstigen. In vorliegender Studie wurde dies am Beispiel der Aphasie erprobt. 15 Schlaganfallpatienten erhielten ein dreiwöchiges Training der selektiven Aufmerksamkeit mit den PC-Programmen CogniPlus und „Konzentration“ bei fünf Sitzungen pro Woche zusätzlich zur Standardtherapie, 13 weitere bildeten eine Kontrollgruppe ohne Aufmerksamkeitstraining. Zur Effektivitätskontrolle dienten zwei Versionen des Untertests Go/Nogo (Testbatterie zur Aufmerksamkeitsprüfung) und die Kurze Aphasieprüfung. Nach dem Training manifestierte sich zwischen den Untersuchungsgruppen kein Unterschied in Aufmerksamkeits- und Sprachfunktionen; das zusätzliche Aufmerksamkeitstraining war also wirkungslos. Allerdings zeigten Patienten mit deutlichen Aufmerksamkeitsverbesserungen tendenziell weniger Aphasie-Symptome, was die Hypothese aufmerksamkeitsvermittelter Plastizitätsmodulation nach Hirnschädigung partiell stützt.

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