scholarly journals 35th ASMS Asilomar Conference on Mass Spectrometry. Mass Spectrometry Imaging: New Developments and Applications

2020 ◽  
Vol 31 (12) ◽  
pp. 2390-2391
Author(s):  
Lingjun Li ◽  
Julia Laskin ◽  
Jeffrey Spraggins
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
New Developments ◽  
Mass Spectrometry Mass Spectrometry ◽  
Asilomar Conference

scholarly journals Kidney Lipidomics by Mass Spectrometry Imaging: A Focus on the Glomerulus

2019 ◽  
Vol 20 (7) ◽  
pp. 1623 ◽  
Author(s):  
Imane Abbas ◽  
Manale Noun ◽  
David Touboul ◽  
Dil Sahali ◽  
Alain Brunelle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Secondary Ion Mass Spectrometry ◽  
Distinct Type ◽  
Kidney Cortex ◽  
New Developments ◽  
Ionization Time ◽  
Lipid Disorders ◽  
Tof Sims ◽  
The Matrix

Lipid disorders have been associated with glomerulopathies, a distinct type of renal pathologies, such as nephrotic syndrome. Global analyses targeting kidney lipids in this pathophysiologic context have been extensively performed, but most often regardless of the architectural and functional complexity of the kidney. The new developments in mass spectrometry imaging technologies have opened a promising field in localized lipidomic studies focused on this organ. In this article, we revisit the main works having employed the Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) technology, and the few reports on the use of TOF-Secondary Ion Mass Spectrometry (TOF-SIMS). We also present a first analysis of mouse kidney cortex sections by cluster TOF-SIMS. The latter represents a good option for high resolution lipid imaging when frozen unfixed histological samples are available. The advantages and drawbacks of this developing field are discussed.

scholarly journals Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study

2021 ◽  
Author(s):  
Riccardo Zecchi ◽  
Pietro Franceschi ◽  
Laura Tigli ◽  
Davide Amidani ◽  
Chiara Catozzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Adult Rabbit ◽  
Chronic Obstructive ◽  
Ionization Mass ◽  
Obstructive Pulmonary Disease ◽  
Imaging Protocol ◽  
Girard’S Reagent

AbstractCorticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard’s reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples. Graphical abstract

scholarly journals Shotgun lipidomics and mass spectrometry imaging unveil diversity and dynamics in Gammarus fossarum lipid composition

iScience ◽  
2021 ◽  
Vol 24 (2) ◽  
pp. 102115
Author(s):  
Tingting Fu ◽  
Oskar Knittelfelder ◽  
Olivier Geffard ◽  
Yohann Clément ◽  
Eric Testet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lipid Composition ◽  
Mass Spectrometry Imaging ◽  
Shotgun Lipidomics ◽  
Gammarus Fossarum
Sign in / Sign up

Export Citation Format

Share Document