scholarly journals Disparity of Cytochrome Utilization in Anodic and Cathodic Extracellular Electron Transfer Pathways of Geobacter sulfurreducens Biofilms

2020 ◽  
Vol 142 (11) ◽  
pp. 5194-5203 ◽  
Author(s):  
Nina Heidary ◽  
Nikolay Kornienko ◽  
Shafeer Kalathil ◽  
Xin Fang ◽  
Khoa H. Ly ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Geobacter Sulfurreducens ◽  
Extracellular Electron Transfer ◽  
Electron Transfer Pathways

scholarly journals Role of Met58 in the regulation of electron/proton transfer in trihaem cytochrome PpcA from Geobacter sulfurreducens

2012 ◽  
Vol 33 (1) ◽  
Author(s):  
Leonor Morgado ◽  
Joana M. Dantas ◽  
Telma Simões ◽  
Yuri Y. Londer ◽  
P. Raj Pokkuluri ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Solvent Accessibility ◽  
Potential Gradient ◽  
Geobacter Sulfurreducens ◽  
Extracellular Electron Transfer ◽  
Redox States ◽  
Electron Transfer Pathways ◽  
Electron Proton Transfer ◽  
Fine Tune

The bacterium Gs (Geobacter sulfurreducens) is capable of oxidizing a large variety of compounds relaying electrons out of the cytoplasm and across the membranes in a process designated as extracellular electron transfer. The trihaem cytochrome PpcA is highly abundant in Gs and is most probably the reservoir of electrons destined for the outer surface. In addition to its role in electron transfer pathways, we have previously shown that this protein could perform e−/H+ energy transduction. This mechanism is achieved by selecting the specific redox states that the protein can access during the redox cycle and might be related to the formation of proton electrochemical potential gradient across the periplasmic membrane. The regulatory role of haem III in the functional mechanism of PpcA was probed by replacing Met58, a residue that controls the solvent accessibility of haem III, with serine, aspartic acid, asparagine or lysine. The data obtained from the mutants showed that the preferred e−/H+ transfer pathway observed for PpcA is strongly dependent on the reduction potential of haem III. It is striking to note that one residue can fine tune the redox states that can be accessed by the trihaem cytochrome enough to alter the functional pathways.

Interaction studies between periplasmic cytochromes provide insights into extracellular electron transfer pathways of Geobacter sulfurreducens

2017 ◽  
Vol 474 (5) ◽  
pp. 797-808 ◽  
Author(s):  
Ana P. Fernandes ◽  
Tiago C. Nunes ◽  
Catarina M. Paquete ◽  
Carlos A. Salgueiro
Keyword(s):  
Electron Transfer ◽  
Geobacter Sulfurreducens ◽  
Extracellular Electron Transfer ◽  
Chemical Shift Perturbation ◽  
Electron Storage ◽  
Surrounding Environment ◽  
Redox Partners ◽  
Soils And Sediments ◽  
Electron Transfer Pathways ◽  
First Time

Geobacter bacteria usually prevail among other microorganisms in soils and sediments where Fe(III) reduction has a central role. This reduction is achieved by extracellular electron transfer (EET), where the electrons are exported from the interior of the cell to the surrounding environment. Periplasmic cytochromes play an important role in establishing an interface between inner and outer membrane electron transfer components. In addition, periplasmic cytochromes, in particular nanowire cytochromes that contain at least 12 haem groups, have been proposed to play a role in electron storage in conditions of an environmental lack of electron acceptors. Up to date, no redox partners have been identified in Geobacter sulfurreducens, and concomitantly, the EET and electron storage mechanisms remain unclear. In this work, NMR chemical shift perturbation measurements were used to probe for an interaction between the most abundant periplasmic cytochrome PpcA and the dodecahaem cytochrome GSU1996, one of the proposed nanowire cytochromes in G. sulfurreducens. The perturbations on the haem methyl signals of GSU1996 and PpcA showed that the proteins form a transient redox complex in an interface that involves haem groups from two different domains located at the C-terminal of GSU1996. Overall, the present study provides for the first time a clear evidence for an interaction between periplasmic cytochromes that might be relevant for the EET and electron storage pathways in G. sulfurreducens.

Diving into the redox properties of Geobacter sulfurreducens cytochromes: a model for extracellular electron transfer

2015 ◽  
Vol 44 (20) ◽  
pp. 9335-9344 ◽  
Author(s):  
Telma C. Santos ◽  
Marta A. Silva ◽  
Leonor Morgado ◽  
Joana M. Dantas ◽  
Carlos A. Salgueiro
Keyword(s):  
Electron Transfer ◽  
Redox Properties ◽  
Geobacter Sulfurreducens ◽  
Extracellular Electron Transfer ◽  
Electron Transfer Pathways

The redox properties of key cytochromes from Geobacter sulfurreducens are used to present an overview for extracellular electron transfer pathways.

Geobacter sulfurreducens adapts to low electrode potential for extracellular electron transfer

2016 ◽  
Vol 191 ◽  
pp. 743-749 ◽  
Author(s):  
Luo Peng ◽  
Xiao-Ting Zhang ◽  
Jie Yin ◽  
Shuo-Yuan Xu ◽  
Yong Zhang ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Electrode Potential ◽  
Geobacter Sulfurreducens ◽  
Extracellular Electron Transfer

Identifying the potential extracellular electron transfer pathways from a c-type cytochrome network

2014 ◽  
Vol 10 (12) ◽  
pp. 3138-3146 ◽  
Author(s):  
De-Wu Ding ◽  
Jun Xu ◽  
Ling Li ◽  
Jian-Ming Xie ◽  
Xiao Sun
Keyword(s):  
Electron Transfer ◽  
Shewanella Oneidensis ◽  
Extracellular Electron Transfer ◽  
Genome Wide ◽  
A Genome ◽  
Electron Transfer Pathways

A genome-wide c-type cytochrome network was constructed to explore the extracellular electron transfer pathways in Shewanella oneidensis MR-1.

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