Bioorthogonal “Labeling after Recognition” Affording an FRET-Based Luminescent Probe for Detecting and Imaging Caspase-3 via Photoluminescence Lifetime Imaging

2019 ◽  
Vol 142 (2) ◽  
pp. 1057-1064 ◽  
Author(s):  
Qi Wu ◽  
Kenneth Yin Zhang ◽  
Peiling Dai ◽  
Hengyu Zhu ◽  
Yun Wang ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Luminescent Probe ◽  
Lifetime Imaging ◽  
Bioorthogonal Labeling ◽  
Photoluminescence Lifetime

Dynamic Monitoring of Phase-Separated Biomolecular Condensates by Photoluminescence Lifetime Imaging

2021 ◽  
Vol 93 (5) ◽  
pp. 2988-2995
Author(s):  
Zihe Yan ◽  
Jianfeng Xue ◽  
Min Zhou ◽  
Jinyu Wang ◽  
Yanxin Zhang ◽  
...  
Keyword(s):  
Dynamic Monitoring ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

scholarly journals A Caspase-3 Reporter for Fluorescence Lifetime Imaging of Single-Cell Apoptosis

Cells ◽  
2018 ◽  
Vol 7 (6) ◽  
pp. 57 ◽  
Author(s):  
Johanna Buschhaus ◽  
Brock Humphries ◽  
Kathryn Luker ◽  
Gary Luker
Keyword(s):  
Single Cell ◽  
Fluorescence Lifetime ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Fluorescence Lifetime Imaging of a Caspase‐3 Apoptosis Reporter

2017 ◽  
Vol 77 (1) ◽  
Author(s):  
Johanna M. Buschhaus ◽  
Anne E. Gibbons ◽  
Kathryn E. Luker ◽  
Gary D. Luker
Keyword(s):  
Fluorescence Lifetime ◽  
Caspase 3 ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging

2015 ◽  
Vol 6 (3) ◽  
pp. 1825-1831 ◽  
Author(s):  
Qiang Zhao ◽  
Xiaobo Zhou ◽  
Tianye Cao ◽  
Kenneth Yin Zhang ◽  
Lijuan Yang ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Tumor Hypoxia ◽  
Lifetime Imaging ◽  
Polymer Dots ◽  
Photoluminescence Lifetime ◽  
Ratiometric Imaging

Fluorescent/phosphorescent dual-emissive conjugated polymer dots were designed and synthesized, ere used for tumor hypoxia sensing via ratiometric imaging and photoluminescence lifetime imaging.

Dynamic photoluminescence lifetime imaging of multicrystalline silicon bricks

2012 ◽  
Vol 106 ◽  
pp. 42-46 ◽  
Author(s):  
Sandra Herlufsen ◽  
Karsten Bothe ◽  
Jan Schmidt ◽  
Rolf Brendel ◽  
Sebastian Siegmund
Keyword(s):  
Multicrystalline Silicon ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

Rational design of a luminescent nanoprobe for hypoxia imaging in vivo via ratiometric and photoluminescence lifetime imaging microscopy

2017 ◽  
Vol 53 (29) ◽  
pp. 4144-4147 ◽  
Author(s):  
Qi Yu ◽  
Tianci Huang ◽  
Yipeng Li ◽  
Huanjie Wei ◽  
Shujuan Liu ◽  
...  
Keyword(s):  
Rational Design ◽  
Imaging Techniques ◽  
Hypoxia Imaging ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

A nanoprobe has been employed for hypoxia imagingin vitroandin vivo viaratiometric and, photoluminescence lifetime imaging techniques.

scholarly journals Fast wide-field upconversion luminescence lifetime thermometry enabled by single-shot compressed ultrahigh-speed imaging

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xianglei Liu ◽  
Artiom Skripka ◽  
Yingming Lai ◽  
Cheng Jiang ◽  
Jingdan Liu ◽  
...  
Keyword(s):  
Upconversion Luminescence ◽  
Wide Field ◽  
Single Shot ◽  
Temperature Mapping ◽  
Optical Instruments ◽  
Lifetime Imaging ◽  
Alternating Direction ◽  
Optical Thermometry ◽  
Photoluminescence Lifetime ◽  
Intensity Decay

AbstractPhotoluminescence lifetime imaging of upconverting nanoparticles is increasingly featured in recent progress in optical thermometry. Despite remarkable advances in photoluminescent temperature indicators, existing optical instruments lack the ability of wide-field photoluminescence lifetime imaging in real time, thus falling short in dynamic temperature mapping. Here, we report video-rate upconversion temperature sensing in wide field using single-shot photoluminescence lifetime imaging thermometry (SPLIT). Developed from a compressed-sensing ultrahigh-speed imaging paradigm, SPLIT first records wide-field luminescence intensity decay compressively in two views in a single exposure. Then, an algorithm, built upon the plug-and-play alternating direction method of multipliers, is used to reconstruct the video, from which the extracted lifetime distribution is converted to a temperature map. Using the core/shell NaGdF4:Er3+,Yb3+/NaGdF4 upconverting nanoparticles as the lifetime-based temperature indicators, we apply SPLIT in longitudinal wide-field temperature monitoring beneath a thin scattering medium. SPLIT also enables video-rate temperature mapping of a moving biological sample at single-cell resolution.

Photoconductance-calibrated photoluminescence lifetime imaging of crystalline silicon

2008 ◽  
Vol 2 (6) ◽  
pp. 245-247 ◽  
Author(s):  
Sandra Herlufsen ◽  
Jan Schmidt ◽  
David Hinken ◽  
Karsten Bothe ◽  
Rolf Brendel
Keyword(s):  
Crystalline Silicon ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

Photoluminescence Lifetime Imaging of Synthesized Proteins in Living Cells Using an Iridium-Alkyne Probe

2017 ◽  
Vol 56 (47) ◽  
pp. 14928-14932 ◽  
Author(s):  
Jinyu Wang ◽  
Jie Xue ◽  
Zihe Yan ◽  
Sichun Zhang ◽  
Juan Qiao ◽  
...  
Keyword(s):  
Living Cells ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime
Sign in / Sign up

Export Citation Format

Share Document