scholarly journals Bioactive Synthetic-Bioinformatic Natural Product Cyclic Peptides Inspired by Nonribosomal Peptide Synthetase Gene Clusters from the Human Microbiome

2019 ◽  
Vol 141 (40) ◽  
pp. 15737-15741 ◽  
Author(s):  
John Chu ◽  
Xavier Vila-Farres ◽  
Sean F. Brady
Keyword(s):  
Natural Product ◽  
Cyclic Peptides ◽  
Human Microbiome ◽  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Nonribosomal Peptide ◽  
Peptide Synthetase

scholarly journals Antimicrobials Inspired by Nonribosomal Peptide Synthetase Gene Clusters

2017 ◽  
Vol 139 (4) ◽  
pp. 1404-1407 ◽  
Author(s):  
Xavier Vila-Farres ◽  
John Chu ◽  
Daigo Inoyama ◽  
Melinda A. Ternei ◽  
Christophe Lemetre ◽  
...  
Keyword(s):  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Nonribosomal Peptide ◽  
Peptide Synthetase

scholarly journals Distribution and Evolution of Nonribosomal Peptide Synthetase Gene Clusters in the Ceratocystidaceae

Genes ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 328 ◽  
Author(s):  
Mohammad Sayari ◽  
Magriet A. van der Nest ◽  
Emma T. Steenkamp ◽  
Nicole C. Soal ◽  
P. Markus Wilken ◽  
...  
Keyword(s):  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Main Element ◽  
Biosynthetic Pathways ◽  
Biosynthetic Gene Clusters ◽  
Nrps Gene ◽  
Nonribosomal Peptide ◽  
Peptide Synthetase ◽  
The Family ◽  
Multifunctional Enzymes

In filamentous fungi, genes in secondary metabolite biosynthetic pathways are generally clustered. In the case of those pathways involved in nonribosomal peptide production, a nonribosomal peptide synthetase (NRPS) gene is commonly found as a main element of the cluster. Large multifunctional enzymes are encoded by members of this gene family that produce a broad spectrum of bioactive compounds. In this research, we applied genome-based identification of nonribosomal peptide biosynthetic gene clusters in the family Ceratocystidaceae. For this purpose, we used the whole genome sequences of species from the genera Ceratocystis, Davidsoniella, Thielaviopsis, Endoconidiophora, Bretziella, Huntiella, and Ambrosiella. To identify and characterize the clusters, different bioinformatics and phylogenetic approaches, as well as PCR-based methods were used. In all genomes studied, two highly conserved NRPS genes (one monomodular and one multimodular) were identified and their potential products were predicted to be siderophores. Expression analysis of two Huntiella species (H. moniliformis and H. omanensis) confirmed the accuracy of the annotations and proved that the genes in both clusters are expressed. Furthermore, a phylogenetic analysis showed that both NRPS genes of the Ceratocystidaceae formed distinct and well supported clades in their respective phylograms, where they grouped with other known NRPSs involved in siderophore production. Overall, these findings improve our understanding of the diversity and evolution of NRPS biosynthetic pathways in the family Ceratocystidaceae.

scholarly journals A genome-wide analysis of nonribosomal peptide synthetase gene clusters and their peptides in a Planktothrix rubescens strain

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 396 ◽  
Author(s):  
Trine B Rounge ◽  
Thomas Rohrlack ◽  
Alexander J Nederbragt ◽  
Tom Kristensen ◽  
Kjetill S Jakobsen
Keyword(s):  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Nonribosomal Peptide ◽  
Genome Wide Analysis ◽  
Planktothrix Rubescens ◽  
Genome Wide ◽  
A Genome ◽  
Peptide Synthetase

scholarly journals Polyketide Synthase and Nonribosomal Peptide Synthetase Gene Clusters in Type Strains of the Genus Phytohabitans

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 257
Author(s):  
Hisayuki Komaki ◽  
Tomohiko Tamura
Keyword(s):  
Secondary Metabolites ◽  
Polyketide Synthase ◽  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Nrps Gene ◽  
Nonribosomal Peptide ◽  
Rare Actinomycetes ◽  
Whole Genomes ◽  
Peptide Synthetase ◽  
Established Genus

(1) Background: Phytohabitans is a recently established genus belonging to rare actinomycetes. It has been unclear if its members have the capacity to synthesize diverse secondary metabolites. Polyketide and nonribosomal peptide compounds are major secondary metabolites in actinomycetes and expected as a potential source for novel pharmaceuticals. (2) Methods: Whole genomes of Phytohabitans flavus NBRC 107702T, Phytohabitans rumicis NBRC 108638T, Phytohabitans houttuyneae NBRC 108639T, and Phytohabitans suffuscus NBRC 105367T were sequenced by PacBio. Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) gene clusters were bioinformatically analyzed in the genome sequences. (3) Results: These four strains harbored 10, 14, 18 and 14 PKS and NRPS gene clusters, respectively. Most of the gene clusters were annotated to synthesis unknown chemistries. (4) Conclusions: Members of the genus Phytohabitans are a possible source for novel and diverse polyketides and nonribosomal peptides.

scholarly journals Genome-based survey of nonribosomal peptide synthetase and polyketide synthase gene clusters in type strains of the genus Microtetraspora

2016 ◽  
Vol 69 (9) ◽  
pp. 712-718 ◽  
Author(s):  
Hisayuki Komaki ◽  
Natsuko Ichikawa ◽  
Tomohiko Tamura ◽  
Akio Oguchi ◽  
Moriyuki Hamada ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Nonribosomal Peptide ◽  
Peptide Synthetase ◽  
Polyketide Synthase Gene ◽  
Type Strains

scholarly journals Active site-directed proteomic probes for adenylation domains in nonribosomal peptide synthetases

2015 ◽  
Vol 51 (12) ◽  
pp. 2262-2265 ◽  
Author(s):  
Sho Konno ◽  
Fumihiro Ishikawa ◽  
Takehiro Suzuki ◽  
Naoshi Dohmae ◽  
Michael D. Burkart ◽  
...  
Keyword(s):  
Natural Product ◽  
Active Site ◽  
Nonribosomal Peptide Synthetase ◽  
Protein Labeling ◽  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases ◽  
Peptide Synthetase ◽  
A Domains

Active site-directed proteomic probes coupled to the 5′-O-N-(aminoacyl)sulfamoyladenosine (AMS) scaffold with a clickable benzophenone functionality selectively target nonribosomal peptide synthetase (NRPS) adenylation (A) domains in natural product producer proteomes by ligand-directed protein labeling.

scholarly journals Draft Genome Sequence of Nonomuraea sp. TP-A0861, a Producer of Myxochelin A

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Hisayuki Komaki ◽  
Natsuko Ichikawa ◽  
Akira Hosoyama ◽  
Nobuyuki Fujita ◽  
Yasuhiro Igarashi
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Draft Genome Sequence ◽  
Nonribosomal Peptide ◽  
Peptide Synthetase

Nonomuraea sp. TP-A0861 produces the nonribosomal peptide myxochelin A, which is known as a microbial siderophore. Here, we report its draft genome sequence. The genome contains at least three nonribosomal peptide synthetase gene clusters, one of which is proposed to be responsible for the biosynthesis of myxochelin A.

scholarly journals Genome based analysis of type-I polyketide synthase and nonribosomal peptide synthetase gene clusters in seven strains of five representative Nocardia species

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 323 ◽  
Author(s):  
Hisayuki Komaki ◽  
Natsuko Ichikawa ◽  
Akira Hosoyama ◽  
Azusa Takahashi-Nakaguchi ◽  
Tetsuhiro Matsuzawa ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Type I ◽  
Nocardia Species ◽  
Nonribosomal Peptide ◽  
Peptide Synthetase
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