scholarly journals Determining Cholesterol Binding to Membrane Proteins by Cholesterol 13C Labeling in Yeast and Dynamic Nuclear Polarization NMR

2018 ◽  
Vol 140 (45) ◽  
pp. 15437-15449 ◽  
Author(s):  
Matthew R. Elkins ◽  
Ivan V. Sergeyev ◽  
Mei Hong
Keyword(s):  
Membrane Proteins ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
13C Labeling ◽  
Cholesterol Binding

scholarly journals Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at protein–protein interfaces

2015 ◽  
Vol 61 (3-4) ◽  
pp. 361-367 ◽  
Author(s):  
Benjamin J. Wylie ◽  
Boris G. Dzikovski ◽  
Shane Pawsey ◽  
Marc Caporini ◽  
Melanie Rosay ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Spin Labels ◽  
Protein Interfaces ◽  
Covalently Bound

scholarly journals Dynamic Nuclear Polarization of Biomembrane Assemblies

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1246
Author(s):  
Nhi T. Tran ◽  
Frédéric Mentink-Vigier ◽  
Joanna R. Long
Keyword(s):  
Membrane Proteins ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Atomic Scale ◽  
Nuclear Spins ◽  
Nmr Studies ◽  
Nmr Characterization ◽  
Recent Developments ◽  
Lipid Assemblies

While atomic scale structural and dynamic information are hallmarks of nuclear magnetic resonance (NMR) methodologies, sensitivity is a fundamental limitation in NMR studies. Fully exploiting NMR capabilities to study membrane proteins is further hampered by their dilution within biological membranes. Recent developments in dynamic nuclear polarization (DNP), which can transfer the relatively high polarization of unpaired electrons to nuclear spins, show promise for overcoming the sensitivity bottleneck and enabling NMR characterization of membrane proteins under native-like conditions. Here we discuss fundamental aspects of DNP-enhanced solid-state NMR spectroscopy, experimental details relevant to the study of lipid assemblies and incorporated proteins, and sensitivity gains which can be realized in biomembrane-based samples. We also present unique insights which can be gained from DNP measurements and prospects for further development of the technique for elucidating structures and orientations of membrane proteins in native lipid environments.

A Method for Dynamic Nuclear Polarization Enhancement of Membrane Proteins

2014 ◽  
Vol 127 (5) ◽  
pp. 1562-1566 ◽  
Author(s):  
Adam N. Smith ◽  
Marc A. Caporini ◽  
Gail E. Fanucci ◽  
Joanna R. Long
Keyword(s):  
Membrane Proteins ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Dynamic Nuclear Polarization Enhancement

A Method for Dynamic Nuclear Polarization Enhancement of Membrane Proteins

2014 ◽  
Vol 54 (5) ◽  
pp. 1542-1546 ◽  
Author(s):  
Adam N. Smith ◽  
Marc A. Caporini ◽  
Gail E. Fanucci ◽  
Joanna R. Long
Keyword(s):  
Membrane Proteins ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Dynamic Nuclear Polarization Enhancement

scholarly journals A biradical-tagged phospholipid as a polarizing agent for solid-state MAS Dynamic Nuclear Polarization NMR of membrane proteins

2019 ◽  
Vol 100 ◽  
pp. 92-101 ◽  
Author(s):  
Daryl B. Good ◽  
Maxim A. Voinov ◽  
David Bolton ◽  
Meaghan E. Ward ◽  
Ivan V. Sergeyev ◽  
...  
Keyword(s):  
Solid State ◽  
Membrane Proteins ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization

Porphyrins as Versatile, Aggregation-Tolerant, and Biocompatible Polarizing Agents for Triplet Dynamic Nuclear Polarization of Biomolecules

2021 ◽  
pp. 2645-2650
Author(s):  
Tomoyuki Hamachi ◽  
Koki Nishimura ◽  
Hironori Kouno ◽  
Yusuke Kawashima ◽  
Kenichiro Tateishi ◽  
...  
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization
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