scholarly journals NRVS Studies of the Peroxide Shunt Intermediate in a Rieske Dioxygenase and Its Relation to the Native FeIIO2Reaction

2018 ◽  
Vol 140 (16) ◽  
pp. 5544-5559 ◽  
Author(s):  
Kyle D. Sutherlin ◽  
Brent S. Rivard ◽  
Lars H. Böttger ◽  
Lei V. Liu ◽  
Melanie S. Rogers ◽  
...  
Keyword(s):  
Rieske Dioxygenase

Development and Application of a High Throughput Assay System for the Detection of Rieske Dioxygenase Activity

2021 ◽  
Author(s):  
Cristina Preston-Herrera ◽  
Aaron S Jackson ◽  
Brian O Bachmann ◽  
Jordan Thomas Froese
Keyword(s):  
High Throughput ◽  
Assay System ◽  
Fluorescent Detection ◽  
System P ◽  
Rieske Dioxygenase ◽  
High Throughput Assay ◽  
Dioxygenase Activity

Herein we report the development of a new periodate-based reactive assay system for the fluorescent detection of the cis-diol metabolites produced by Rieske dioxygenases. This sensitive and diastereoselective assay system...

scholarly journals The Generation of the Oxidant Agent of a Mononuclear Nonheme Fe(II) Biomimetic Complex by Oxidative Decarboxylation. A DFT Investigation

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 328
Author(s):  
Angela Parise ◽  
Maria Costanza Muraca ◽  
Nino Russo ◽  
Marirosa Toscano ◽  
Tiziana Marino
Keyword(s):  
Potential Energy Surfaces ◽  
Density Functional ◽  
Complex Model ◽  
Hydroxyl Group ◽  
Oxidative Decarboxylation ◽  
Acid Complex ◽  
Rate Determining Step ◽  
Electronic Spin ◽  
Rieske Dioxygenase ◽  
Energy Surfaces

The oxidative decarboxylation of the iron(II) α-hydroxy acid (mandelic acid) complex model, biomimetic of Rieske dioxygenase, has been investigated at the density functional level. The explored mechanism sheds light on the role of the α-hydroxyl group on the dioxygen activation. The potential energy surfaces have been explored in different electronic spin states. The rate-determining step of the process is the proton transfer. The oxidative decarboxylation preferentially takes place on the quintet state.

Benzoate 1,2-Dioxygenase fromPseudomonas putida:  Single Turnover Kinetics and Regulation of a Two-Component Rieske Dioxygenase†

Biochemistry ◽  
2002 ◽  
Vol 41 (30) ◽  
pp. 9611-9626 ◽  
Author(s):  
Matt D. Wolfe ◽  
Daniel J. Altier ◽  
Audria Stubna ◽  
Codrina V. Popescu ◽  
Eckard Münck ◽  
...  
Keyword(s):  
Single Turnover ◽  
Two Component ◽  
Rieske Dioxygenase
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