Engineering Bifunctional Antibodies with Constant Region Fusion Architectures

2017 ◽  
Vol 139 (51) ◽  
pp. 18607-18615 ◽  
Author(s):  
Juanjuan Du ◽  
Yu Cao ◽  
Yan Liu ◽  
Ying Wang ◽  
Yong Zhang ◽  
...  
Keyword(s):  
Constant Region ◽  
Bifunctional Antibodies

scholarly journals Variance analysis of immunoglobulin alleles in natural populations of rabbit (Oryctolagus cuniculus): the extensive interallelic divergence at the b locus could be the outcome of overdominance-type selection.

Genetics ◽  
1993 ◽  
Vol 135 (1) ◽  
pp. 171-187 ◽  
Author(s):  
W van der Loo
Keyword(s):  
Heavy Chain ◽  
Light Chain ◽  
Gene Diversity ◽  
Natural Populations ◽  
Constant Region ◽  
Immunoglobulin Light Chain ◽  
Evolutionary Patterns ◽  
B Locus ◽  
Type Selection ◽  
Predominant Allele

Abstract Population genetic data are presented which should contribute to evaluation of the hypothesis that the extraordinary evolutionary patterns observed at the b locus of the rabbit immunoglobulin light chain constant region can be the outcome of overdominance-type selection. The analysis of allele correlations in natural populations revealed an excess of heterozygotes of about 10% at the b locus while heterozygote excess was not observed at loci determining the immunoglobulin heavy chain. Data from the published literature, where homozygote advantage was suggested, were reevaluated and found in agreement with data here presented. Gene diversity was evenly distributed among populations and showed similarities with patterns reported for histocompatibility loci. Analysis of genotypic disequilibria revealed strong digenic associations between the leading alleles of heavy and light chain constant region loci in conjunction with trigenic disequilibria corresponding to a preferential association of b locus heterozygosity with the predominant allele of the heavy chain e locus. It is argued that this may indicate compensatory or nonadditive aspects of a putative heterozygosity enhancing mechanism, implying that effects at the light chain might be more pronounced in populations fixed for the heavy chain polymorphism.

scholarly journals Anti-immunoglobulin antibodies. I. Expression of cross-reactive idiotypes and Ir gene control of the response to IgG2a of the b allotype.

1980 ◽  
Vol 151 (6) ◽  
pp. 1334-1348 ◽  
Author(s):  
C Bona ◽  
P K Mongini ◽  
K E Stein ◽  
W E Paul
Keyword(s):  
Poor Response ◽  
Domain Specificity ◽  
Gene Control ◽  
Constant Region ◽  
Myeloma Protein ◽  
Histocompatibility Complex ◽  
Heavy Chain Constant Region ◽  
Ch2 Domain ◽  
Ig Heavy Chain ◽  
Ir Genes

The anti-allotype antibody response to the b allotypic form of IgG2a is regulated by major histocompatibility complex (MHC)-encoded immune response (Ir) genes. Mice of d, b, p, q, r, and s haplotypes make a strong anti-allotype response on immunization with the CBPC101 myeloma protein (IgG2ab), whereas mice of the k, m, a, a1, u, and z haplotypes made no, or a very poor, response. All responder strains produce anti-IgG2ab antibodies which share common idiotypes (Id) without relation to the allelic forms of the Ig heavy-chain-constant region genes that the responding mice possess. Isoelectric focusing analysis of the anti-allotype antibodies produced in various strains of mice showed that they are of limited heterogeneity and quite similar from strain to strain. Five out of six hybridoma products with specificity for CBPC101 allotype expressed cross-reactive idiotypes (IdX). Two of hybridoma products expressing IdX identify CH3-domain determinants, and one has been assigned a CH2-domain specificity.

Production of the human immunoglobulin γ1 chain constant region polypeptides in Escherichia coli

1988 ◽  
Vol 8 (2) ◽  
pp. 141-148 ◽  
Author(s):  
Satoshi Nakamura ◽  
Tsukio Masegi ◽  
Kazuo Kitai ◽  
Akira Kudo ◽  
Takeshi Watanabe ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Immunoglobulin ◽  
Constant Region

scholarly journals LB783 Skin-tethered bifunctional antibodies for treatment of autoimmune and inflammatory skin diseases

2021 ◽  
Vol 141 (9) ◽  
pp. B18
Author(s):  
P. Mande ◽  
S. Borthakur ◽  
D. Rios ◽  
P. Halvey ◽  
A. Boisvert ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Inflammatory Skin Diseases ◽  
Inflammatory Skin ◽  
Bifunctional Antibodies

Effects of mutation position on frequency of marker rescue by homologous recombination

1992 ◽  
Vol 12 (8) ◽  
pp. 3609-3613
Author(s):  
L Jiang ◽  
A Connor ◽  
M J Shulman
Keyword(s):  
Homologous Recombination ◽  
Normal Function ◽  
Constant Region ◽  
Dna Fragments ◽  
Wild Type ◽  
End Effect ◽  
Chromosomal Dna ◽  
Base Pairs ◽  
Marker Rescue ◽  
Immunoglobulin Mu

Homologous recombination between transferred and chromosomal DNA can be used for mapping mutations by marker rescue, i.e., by identifying which segment of wild-type DNA can recombine with the mutant chromosomal gene and restore normal function. In order to define how much the fragments should overlap each other for reliable mapping, we have measured how the frequency of marker rescue is affected by the position of the chromosomal mutation relative to the ends of the transferred DNA fragments. For this purpose, we used several DNA fragments to effect marker rescue in two mutant hybridomas which bear mutations 673 bp apart in the exons encoding the second and third constant region domains of the immunoglobulin mu heavy chain. The frequency of marker rescue decreased greatly when the mutation was located near one of the ends of the fragments, the results indicating that fragments should be designed to overlap by at least several hundred base pairs. Possible explanations for this "end effect" are considered.

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