Cell-Surface Receptor–Ligand Interaction Analysis with Homogeneous Time-Resolved FRET and Metabolic Glycan Engineering: Application to Transmembrane and GPI-Anchored Receptors

2017 ◽  
Vol 139 (46) ◽  
pp. 16822-16829 ◽  
Author(s):  
Henning Stockmann ◽  
Viktor Todorovic ◽  
Paul L. Richardson ◽  
Violeta Marin ◽  
Victoria Scott ◽  
...  
Keyword(s):  
Cell Surface ◽  
Interaction Analysis ◽  
Engineering Application ◽  
Cell Surface Receptor ◽  
Ligand Interaction ◽  
Receptor Ligand ◽  
Time Resolved ◽  
Surface Receptor

scholarly journals Synthesis of end-labeled multivalent ligands for exploring cell-surface-receptor–ligand interactions

2000 ◽  
Vol 7 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Eva J Gordon ◽  
Jason E Gestwicki ◽  
Laura E Strong ◽  
Laura L Kiessling
Keyword(s):  
Cell Surface ◽  
Cell Surface Receptor ◽  
Receptor Ligand ◽  
Multivalent Ligands ◽  
Ligand Interactions ◽  
Surface Receptor

Development and Characterization of a High-Throughput System for Assessing Cell-Surface Receptor−Ligand Engagement

Langmuir ◽  
2005 ◽  
Vol 21 (18) ◽  
pp. 8374-8384 ◽  
Author(s):  
G. M. Harbers ◽  
L. J. Gamble ◽  
E. F. Irwin ◽  
D. G. Castner ◽  
K. E. Healy
Keyword(s):  
Cell Surface ◽  
High Throughput ◽  
Cell Surface Receptor ◽  
Receptor Ligand ◽  
Surface Receptor

scholarly journals Evidence that the thyrotropin-releasing hormone receptor and its ligand are recycled dissociated from each other

1995 ◽  
Vol 306 (1) ◽  
pp. 107-113 ◽  
Author(s):  
C P Petrou ◽  
A H Tashjian
Keyword(s):  
Cell Surface ◽  
Hormone Receptor ◽  
Thyrotropin Releasing Hormone ◽  
Cell Surface Receptor ◽  
Acidic Ph ◽  
Receptor Recycling ◽  
Bafilomycin A1 ◽  
Receptor Ligand ◽  
Surface Receptor ◽  
Receptor Pool

We have examined the trafficking of the thyrotropin-releasing hormone receptor (TRHR) and its ligand, after TRHR-TRH internalization in rat pituitary GH4C1 cells. After rapid ligand-induced receptor sequestration, the cell surface receptor pool was replenished. Replenishment was insensitive to inhibition of protein synthesis and was dependent on the duration of internalization; therefore, the replenished receptors were not newly synthesized but recycled. The total amount of recycled receptors decreased with increasing internalization time, resulting in only partial replenishment of the cell-surface receptor pool after prolonged incubation with ligand. Thus, in addition to a receptor recycling pathway, a non-cycling route exists for TRHR sorting; this route became dominant with increasing internalization periods. TRHR entry into these pathways was not determined by the affinity of the receptor-ligand interaction, because the extent of receptor recycling was similar after TRH- and methyl-TRH (MeTRH)-induced internalization. Unlike results with the TRHR, the TRH recycling pool was not depleted by the noncycling pathway. After multiple rounds of [3H]MeTRH internalization, the amount of cell-associated radioactivity increased with increasing internalization time due to accumulation of the ligand or its metabolites in a non-cycling pathway, but the absolute amount of recycled ligand remained constant after short or long internalization times. The difference in the proportion of TRHR and MeTRH that were diverted into a noncycling pathway indicated intracellular dissociation of the internalized TRHR-TRH complex. Dissociation of the internalized TRHR-TRH complex was dependent on the acidic pH in an intracellular compartment. Although extracellular acidic pH did not enhance cell-surface receptor-ligand (RL) dissociation, bafilomycin A1 inhibited both receptor and ligand recycling. We conclude that the TRHR-TRH system is unique among recycling receptors because, after RL sequestration, the TRHR-TRH complex becomes dissociated intracellularly via a bafilomycin A1-sensitive, acidic pH-dependent mechanism, and both the unoccupied TRHR and TRH recycle disassociated from each other.

Expression, function, and localization of the REG cell surface receptor

2001 ◽  
Vol 120 (5) ◽  
pp. A18-A19
Author(s):  
B DIECKGRAEFE ◽  
C HOUCHEN ◽  
H ZHANG
Keyword(s):  
Cell Surface ◽  
Cell Surface Receptor ◽  
Surface Receptor
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