Allosteric Control of Oxidative Catalysis by a DNA Rotaxane Nanostructure

2017 ◽  
Vol 139 (45) ◽  
pp. 16044-16047 ◽  
Author(s):  
Mathias Centola ◽  
Julián Valero ◽  
Michael Famulok
Keyword(s):  
Allosteric Control ◽  
Oxidative Catalysis

scholarly journals Rab1-AMPylation by Legionella DrrA is allosterically activated by Rab1

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jiqing Du ◽  
Marie-Kristin von Wrisberg ◽  
Burak Gulen ◽  
Matthias Stahl ◽  
Christian Pett ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Adenosine Monophosphate ◽  
Vesicular Trafficking ◽  
Bacterial Protein ◽  
Post Translational Modification ◽  
Allosteric Control ◽  
Rab Protein ◽  
Biochemical Characterisation ◽  
A Site ◽  
Insight Into

AbstractLegionella pneumophila infects eukaryotic cells by forming a replicative organelle – the Legionella containing vacuole. During this process, the bacterial protein DrrA/SidM is secreted and manipulates the activity and post-translational modification (PTM) states of the vesicular trafficking regulator Rab1. As a result, Rab1 is modified with an adenosine monophosphate (AMP), and this process is referred to as AMPylation. Here, we use a chemical approach to stabilise low-affinity Rab:DrrA complexes in a site-specific manner to gain insight into the molecular basis of the interaction between the Rab protein and the AMPylation domain of DrrA. The crystal structure of the Rab:DrrA complex reveals a previously unknown non-conventional Rab-binding site (NC-RBS). Biochemical characterisation demonstrates allosteric stimulation of the AMPylation activity of DrrA via Rab binding to the NC-RBS. We speculate that allosteric control of DrrA could in principle prevent random and potentially cytotoxic AMPylation in the host, thereby perhaps ensuring efficient infection by Legionella.

scholarly journals Conformational equilibria in allosteric control of Hsp70 chaperones

2021 ◽  
Vol 81 (19) ◽  
pp. 3919-3933.e7
Author(s):  
Wei Wang ◽  
Qinglian Liu ◽  
Qun Liu ◽  
Wayne A. Hendrickson
Keyword(s):  
Allosteric Control ◽  
Conformational Equilibria

scholarly journals Conformational changes involving ammonia tunnel formation and allosteric control in GMP synthetase

2014 ◽  
Vol 545 ◽  
pp. 22-32 ◽  
Author(s):  
Justin C. Oliver ◽  
Ravidra Gudihal ◽  
John W. Burgner ◽  
Anthony M. Pedley ◽  
Alexander T. Zwierko ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Allosteric Control ◽  
Ammonia Tunnel ◽  
Gmp Synthetase

Engineering an Allosteric Control of Protein Function

2021 ◽  
Vol 125 (7) ◽  
pp. 1806-1814
Author(s):  
Yashavantha L. Vishweshwaraiah ◽  
Jiaxing Chen ◽  
Nikolay V. Dokholyan
Keyword(s):  
Protein Function ◽  
Allosteric Control

scholarly journals DNA-Induced Allosteric Control Regulates Activation of Cas12A

2021 ◽  
Vol 120 (3) ◽  
pp. 17a
Author(s):  
Pablo R. Arantes ◽  
Aakash Saha ◽  
Martin Jinek ◽  
Giulia Palermo
Keyword(s):  
Allosteric Control

scholarly journals Targeting Allosteric Control Mechanisms in Heat Shock Protein 70 (Hsp70)

2016 ◽  
Vol 16 (25) ◽  
pp. 2729-2740 ◽  
Author(s):  
Xiaokai Li ◽  
Hao Shao ◽  
Isabelle R. Taylor ◽  
Jason E. Gestwicki
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Control Mechanisms ◽  
Allosteric Control
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