scholarly journals Monitoring Hydrogen Exchange During Protein Folding by Fast Pressure Jump NMR Spectroscopy

2017 ◽  
Vol 139 (32) ◽  
pp. 11036-11039 ◽  
Author(s):  
T. Reid Alderson ◽  
Cyril Charlier ◽  
Dennis A. Torchia ◽  
Philip Anfinrud ◽  
Ad Bax
Keyword(s):  
Protein Folding ◽  
Nmr Spectroscopy ◽  
Hydrogen Exchange ◽  
Pressure Jump

scholarly journals Base-Pair Opening Dynamics Study of Fluoride Riboswitch in the Bacillus cereus CrcB Gene

2021 ◽  
Vol 22 (6) ◽  
pp. 3234
Author(s):  
Juhyun Lee ◽  
Si-Eun Sung ◽  
Janghyun Lee ◽  
Jin Young Kang ◽  
Joon-Hwa Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Regulation ◽  
Nmr Spectroscopy ◽  
Bacillus Cereus ◽  
Base Pair ◽  
Noncoding Rna ◽  
Hydrogen Exchange ◽  
Magnesium Ion ◽  
Fluoride Ion ◽  
Base Pair Opening

Riboswitches are segments of noncoding RNA that bind with metabolites, resulting in a change in gene expression. To understand the molecular mechanism of gene regulation in a fluoride riboswitch, a base-pair opening dynamics study was performed with and without ligands using the Bacillus cereus fluoride riboswitch. We demonstrate that the structural stability of the fluoride riboswitch is caused by two steps depending on ligands. Upon binding of a magnesium ion, significant changes in a conformation of the riboswitch occur, resulting in the greatest increase in their stability and changes in dynamics by a fluoride ion. Examining hydrogen exchange dynamics through NMR spectroscopy, we reveal that the stabilization of the U45·A37 base-pair due to the binding of the fluoride ion, by changing the dynamics while maintaining the structure, results in transcription regulation. Our results demonstrate that the opening dynamics and stabilities of a fluoride riboswitch in different ion states are essential for the genetic switching mechanism.

Cooperativity of a Protein Folding Reaction Probed at Multiple Chain Positions by Real-Time 2D NMR Spectroscopy†

Biochemistry ◽  
2000 ◽  
Vol 39 (27) ◽  
pp. 7910-7919 ◽  
Author(s):  
Clemens Steegborn ◽  
Henriette Schneider-Hassloff ◽  
Markus Zeeb ◽  
Jochen Balbach
Keyword(s):  
Protein Folding ◽  
Nmr Spectroscopy ◽  
Real Time ◽  
2D Nmr ◽  
2D Nmr Spectroscopy ◽  
Multiple Chain

scholarly journals Monitoring 15N Chemical Shifts During Protein Folding by Pressure-Jump NMR

2018 ◽  
Vol 140 (26) ◽  
pp. 8096-8099 ◽  
Author(s):  
Cyril Charlier ◽  
Joseph M. Courtney ◽  
T. Reid Alderson ◽  
Philip Anfinrud ◽  
Ad Bax
Keyword(s):  
Protein Folding ◽  
Chemical Shifts ◽  
Pressure Jump

Kinetic studies of protein folding using NMR spectroscopy

10.1038/744 ◽  
1998 ◽  
Vol 5 (7) ◽  
pp. 504-507 ◽  
Author(s):  
Christopher M. Dobson ◽  
Peter J. Hore
Keyword(s):  
Protein Folding ◽  
Nmr Spectroscopy ◽  
Kinetic Studies
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