Photostable Ratiometric Pdot Probe for in Vitro and in Vivo Imaging of Hypochlorous Acid

: Ghrelin is a 28-amino acid octanoylated peptide hormone that is implicated in many physiological and pathophysiological processes. Specific visualization of ghrelin and its cognate receptor using traceable ligands is crucial in elucidating the localization, functions, and expression pattern of the peptide’s signaling pathway. Here 12 representative radio- and fluorescently-labeled peptide-based ligands are reviewed for in vitro and in vivo imaging studies. In particular, the focus is on their structural features, pharmacological properties, and applications in further biochemical research.

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Molecular imaging and deep learning analysis of uMUC1 expression in response to chemotherapy in an orthotopic model of ovarian cancer

Scientific Reports ◽

10.1038/s41598-020-71890-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Hongwei Zhao ◽

Hasaan Hayat ◽

Xiaohong Ma ◽

Daguang Fan ◽

Ping Wang ◽

...

Keyword(s):

Neural Networks ◽

Ovarian Cancer ◽

Deep Learning ◽

Cancer Progression ◽

In Vivo Imaging ◽

Near Infrared ◽

Response To Therapy ◽

Response To Chemotherapy

Abstract Artificial Intelligence (AI) algorithms including deep learning have recently demonstrated remarkable progress in image-recognition tasks. Here, we utilized AI for monitoring the expression of underglycosylated mucin 1 (uMUC1) tumor antigen, a biomarker for ovarian cancer progression and response to therapy, using contrast-enhanced in vivo imaging. This was done using a dual-modal (magnetic resonance and near infrared optical imaging) uMUC1-specific probe (termed MN-EPPT) consisted of iron-oxide magnetic nanoparticles (MN) conjugated to a uMUC1-specific peptide (EPPT) and labeled with a near-infrared fluorescent dye, Cy5.5. In vitro studies performed in uMUC1-expressing human ovarian cancer cell line SKOV3/Luc and control uMUC1low ES-2 cells showed preferential uptake on the probe by the high expressor (n = 3, p < .05). A decrease in MN-EPPT uptake by SKOV3/Luc cells in vitro due to uMUC1 downregulation after docetaxel therapy was paralleled by in vivo imaging studies that showed a reduction in probe accumulation in the docetaxel treated group (n = 5, p < .05). The imaging data were analyzed using deep learning-enabled segmentation and quantification of the tumor region of interest (ROI) from raw input MRI sequences by applying AI algorithms including a blend of Convolutional Neural Networks (CNN) and Fully Connected Neural Networks. We believe that the algorithms used in this study have the potential to improve studying and monitoring cancer progression, amongst other diseases.

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In Vitro and In Vivo Imaging of Peptide-Encapsulated Polymer Nanoparticles for Cancer Biomarker Activated Drug Delivery

IEEE Transactions on NanoBioscience ◽

10.1109/tnb.2013.2274781 ◽

2013 ◽

Vol 12 (4) ◽

pp. 304-310 ◽

Cited By ~ 6

Author(s):

Gulsim K. Kulsharova ◽

Matthew B. Lee ◽

Felice Cheng ◽

Munima Haque ◽

Hyungsoo Choi ◽

...

Keyword(s):

Drug Delivery ◽

In Vivo Imaging ◽

Cancer Biomarker ◽

Polymer Nanoparticles

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Combining in Vitro Diagnostics with in Vivo Imaging for Earlier Detection of Pancreatic Ductal Adenocarcinoma: Challenges and Solutions

Radiology ◽

10.1148/radiol.2015141020 ◽

2015 ◽

Vol 277 (3) ◽

pp. 644-661 ◽

Cited By ~ 15

Author(s):

Paul F. Laeseke ◽

Ru Chen ◽

R. Brooke Jeffrey ◽

Teresa A. Brentnall ◽

Jürgen K. Willmann

Keyword(s):

Pancreatic Ductal Adenocarcinoma ◽

In Vivo Imaging ◽

Ductal Adenocarcinoma ◽

In Vitro Diagnostics

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Time-resolved diffuse optical tomography and its application to in vitro and in vivo imaging

Journal of Biomedical Optics ◽

10.1117/1.2815724 ◽

2007 ◽

Vol 12 (6) ◽

pp. 062107 ◽

Cited By ~ 30

Author(s):

Huijuan Zhao ◽

Feng Gao ◽

Yukari Tanikawa ◽

Yukio Yamada

Keyword(s):

In Vivo Imaging ◽

Optical Tomography ◽

Diffuse Optical Tomography ◽

Time Resolved

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From In Vitro to In Vivo: Imaging from the Single Cell to the Whole Organism

Current Protocols in Cytometry ◽

10.1002/0471142956.cy1212s44 ◽

2008 ◽

Vol 44 (1) ◽

Cited By ~ 2

Author(s):

Jung Julie Kang ◽

Ildiko Toma ◽

Arnold Sipos ◽

Janos Peti‐Peterdi

Keyword(s):

Single Cell ◽

In Vivo Imaging

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Using in vitro lipolysis and SPECT/CT in vivo imaging to understand oral absorption of fenofibrate from lipid-based drug delivery systems

Journal of Controlled Release ◽

10.1016/j.jconrel.2019.11.024 ◽

2020 ◽

Vol 317 ◽

pp. 375-384 ◽

Cited By ~ 2

Author(s):

Thuy Tran ◽

Peter Bønløkke ◽

Cristina Rodríguez-Rodríguez ◽

Zeynab Nosrati ◽

Pedro Luis Esquinas ◽

...

Keyword(s):

Drug Delivery ◽

In Vivo Imaging ◽

Drug Delivery Systems ◽

Oral Absorption ◽

Delivery Systems ◽

In Vitro Lipolysis

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Assessing the Efficacy of Dietary Selenomethionine Supplementation in the Setting of Cardiac Ischemia/Reperfusion Injury

Antioxidants ◽

10.3390/antiox8110546 ◽

2019 ◽

Vol 8 (11) ◽

pp. 546 ◽

Cited By ~ 2

Author(s):

Leila Reyes ◽

David P. Bishop ◽

Clare L. Hawkins ◽

Benjamin S. Rayner

Keyword(s):

Cardiac Myocyte ◽

Hypochlorous Acid ◽

Ischemia Reperfusion Injury ◽

Heart Function ◽

Ischemia Reperfusion ◽

Cytosolic Calcium ◽

Cardiac Ischemia ◽

Cellular Damage

Oxidative stress is a major hallmark of cardiac ischemia/reperfusion (I/R) injury. This partly arises from the presence of activated phagocytes releasing myeloperoxidase (MPO) and its production of hypochlorous acid (HOCl). The dietary supplement selenomethionine (SeMet) has been shown to bolster endogenous antioxidant processes as well as readily react with MPO-derived oxidants. The aim of this study was to assess whether supplementation with SeMet could modulate the extent of cellular damage observed in an in vitro cardiac myocyte model exposed to (patho)-physiological levels of HOCl and an in vivo rat model of cardiac I/R injury. Exposure of the H9c2 cardiac myoblast cell line to HOCl resulted in a dose-dependent increase in necrotic cell death, which could be prevented by SeMet supplementation and was attributed to SeMet preventing the HOCl-induced loss of mitochondrial inner trans-membrane potential, and the associated cytosolic calcium accumulation. This protection was credited primarily to the direct oxidant scavenging ability of SeMet, with a minor contribution arising from the ability of SeMet to bolster cardiac myoblast glutathione peroxidase (GPx) activity. In vivo, a significant increase in selenium levels in the plasma and heart tissue were seen in male Wistar rats fed a diet supplemented with 2 mg kg−1 SeMet compared to controls. However, SeMet-supplementation demonstrated only limited improvement in heart function and did not result in better heart remodelling following I/R injury. These data indicate that SeMet supplementation is of potential benefit within pathological settings where excessive HOCl is known to be generated but has limited efficacy as a therapeutic agent for the treatment of heart attack.

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