Structural Model of the Tubular Assembly of the Rous Sarcoma Virus Capsid Protein

2017 ◽  
Vol 139 (5) ◽  
pp. 2006-2013 ◽  
Author(s):  
Jaekyun Jeon ◽  
Xin Qiao ◽  
Ivan Hung ◽  
Alok K. Mitra ◽  
Ambroise Desfosses ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Structural Model ◽  
Virus Capsid ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Virus Capsid Protein

scholarly journals Crystals of Rous Sarcoma Virus Capsid Protein Show a Helical Arrangement of Protein Subunits

Virology ◽  
1997 ◽  
Vol 238 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Ladislau C Kovari ◽  
Cory A Momany ◽  
Faith Miyagi ◽  
Sukyeong Lee ◽  
Stephen Campbell ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Virus Capsid ◽  
Protein Subunits ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Virus Capsid Protein

Analysis of rous sarcoma virus capsid protein variants assembled on lipid monolayers

2002 ◽  
Vol 316 (3) ◽  
pp. 667-678 ◽  
Author(s):  
Keith Mayo ◽  
Marcy L Vana ◽  
Jason McDermott ◽  
Doug Huseby ◽  
Jonathan Leis ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Lipid Monolayers ◽  
Virus Capsid ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Protein Variants ◽  
Virus Capsid Protein

scholarly journals Structure and self-association of the Rous sarcoma virus capsid protein

Structure ◽  
2000 ◽  
Vol 8 (6) ◽  
pp. 617-628 ◽  
Author(s):  
Richard L Kingston ◽  
Tanja Fitzon-Ostendorp ◽  
Elan Zohar Eisenmesser ◽  
Gisela W Schatz ◽  
Volker M Vogt ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Virus Capsid ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Self Association ◽  
Virus Capsid Protein

Dimeric Rous Sarcoma Virus Capsid Protein Structure Relevant to Immature Gag Assembly

2004 ◽  
Vol 335 (1) ◽  
pp. 275-282 ◽  
Author(s):  
Narayanasamy Nandhagopal ◽  
Alan A. Simpson ◽  
Marc C. Johnson ◽  
Adam B. Francisco ◽  
Gisela W. Schatz ◽  
...  
Keyword(s):  
Protein Structure ◽  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Virus Capsid ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Gag Assembly ◽  
Virus Capsid Protein

scholarly journals Proton-driven Assembly of the Rous Sarcoma Virus Capsid Protein Results in the Formation of Icosahedral Particles

2010 ◽  
Vol 285 (20) ◽  
pp. 15056-15064 ◽  
Author(s):  
Jae-Kyung Hyun ◽  
Mazdak Radjainia ◽  
Richard L. Kingston ◽  
Alok K. Mitra
Keyword(s):  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Virus Capsid ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Virus Capsid Protein

scholarly journals In vitro assembly of the Rous Sarcoma Virus capsid protein into hexamer tubes at physiological temperature

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Soumeya A. Jaballah ◽  
Graham D. Bailey ◽  
Ambroise Desfosses ◽  
Jaekyung Hyun ◽  
Alok K. Mitra ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Virus Capsid ◽  
Physiological Temperature ◽  
Rous Sarcoma ◽  
In Vitro Assembly ◽  
Sarcoma Virus ◽  
Virus Capsid Protein

scholarly journals Structural studies of the rous sarcoma virus (RSV) capsid protein

1996 ◽  
Vol 52 (a1) ◽  
pp. C181-C181
Author(s):  
L. C. Kovari ◽  
C. Momany ◽  
F. Miyagi ◽  
R. Zhao ◽  
S. Campbell ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Structural Studies ◽  
Rous Sarcoma ◽  
Sarcoma Virus

scholarly journals In vitroassembly of the Rous Sarcoma Virus capsid protein into hexamer tubes under physiological conditions

2017 ◽  
Author(s):  
Soumeya A. Jaballah ◽  
Graham D. Bailey ◽  
Ambroise Desfosses ◽  
Jaekyung Hyun ◽  
Alok K. Mitra ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rous Sarcoma Virus ◽  
Tube Formation ◽  
Maturation Process ◽  
Rous Sarcoma ◽  
C Terminus ◽  
Sarcoma Virus ◽  
Tube Assembly ◽  
A Minor

ABSTRACTDuring a proteolytically-driven maturation process, the ortho-retroviral capsid protein (CA) assembles to form the convex shell that surrounds the viral genome. In some orthoretroviruses, including Rous Sarcoma Virus (RSV), CA carries a short and hydrophobic spacer peptide (SP) at its C-terminus early in the maturation process, which is progressively removed as maturation proceeds. In this work, we show that RSV CA assemblesin vitroat physiological temperatures, forming hexamer tubes that effectively model the mature capsid surface. Tube assembly is strongly influenced by electrostatic effects, and is a nucleated process that remains thermodynamically favored at lower temperatures, but is effectively arrested by the large Gibbs energy barrier associated with nucleation. RSV CA tubes are multi-layered, being formed by nested and concentric tubes of capsid hexamers. However the spacer peptide acts as a layering determinant during tube assembly. If only a minor fraction of CA-SP is present, multi-layered tube formation is blocked, and single-layered tubes predominate. This likely prevents formation of biologically aberrant multi-layered capsids in the virion. The generation of single-layered hexamer tubes facilitated 3D helical image reconstruction from cryo-electron microscopy data, revealing the basic tube architecture.

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