Effective Antisense Gene Regulation via Noncationic, Polyethylene Glycol Brushes

2016 ◽  
Vol 138 (29) ◽  
pp. 9097-9100 ◽  
Author(s):  
Xueguang Lu ◽  
Fei Jia ◽  
Xuyu Tan ◽  
Dali Wang ◽  
Xueyan Cao ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Polyethylene Glycol ◽  
Antisense Gene

scholarly journals Precision Tuning of DNA- and Poly(ethylene glycol)-Based Nanoparticles via Coassembly for Effective Antisense Gene Regulation

2017 ◽  
Vol 29 (23) ◽  
pp. 9882-9886 ◽  
Author(s):  
Dali Wang ◽  
Xueguang Lu ◽  
Fei Jia ◽  
Xuyu Tan ◽  
Xiaoya Sun ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Antisense Gene ◽  
Poly Ethylene

Biocompatible Infinite-Coordination-Polymer Nanoparticle-Nucleic-Acid Conjugates for Antisense Gene Regulation

2014 ◽  
Vol 127 (2) ◽  
pp. 486-490 ◽  
Author(s):  
Colin M. Calabrese ◽  
Timothy J. Merkel ◽  
William E. Briley ◽  
Pratik S. Randeria ◽  
Suguna P. Narayan ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Nucleic Acid ◽  
Coordination Polymer ◽  
Antisense Gene ◽  
Polymer Nanoparticle

scholarly journals Biocompatible Infinite-Coordination-Polymer Nanoparticle-Nucleic-Acid Conjugates for Antisense Gene Regulation

2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
Colin M. Calabrese ◽  
Timothy J. Merkel ◽  
William E. Briley ◽  
Pratik S. Randeria ◽  
Suguna P. Narayan ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Nucleic Acid ◽  
Coordination Polymer ◽  
Antisense Gene ◽  
Polymer Nanoparticle

Alternative polyadenylation of antisense RNAs and flowering time control

2010 ◽  
Vol 38 (4) ◽  
pp. 1077-1081 ◽  
Author(s):  
Csaba Hornyik ◽  
Céline Duc ◽  
Katarzyna Rataj ◽  
Lionel C. Terzi ◽  
Gordon G. Simpson
Keyword(s):  
Gene Regulation ◽  
Flowering Time ◽  
Alternative Polyadenylation ◽  
Antisense Gene ◽  
Antisense Rnas ◽  
Alternative Processing ◽  
Time Control ◽  
Cleavage And Polyadenylation ◽  
Floral Repressor ◽  
Flowering Time Control

Flowering time is controlled by precision in gene regulation mediated by different pathways. Two Arabidopsis thaliana components of the autonomous flowering pathway, FCA and FPA, function as genetically independent trans-acting regulators of alternative cleavage and polyadenylation. FCA and FPA directly associate with chromatin at the locus encoding the floral repressor FLC, but appear to control FLC transcription by mediating alternative polyadenylation of embedded non-coding antisense RNAs. These findings prompt the re-examination of how other factors control FLC expression, as it is formally possible that they function primarily to control alternative processing of antisense RNAs. As co-expressed sense and antisense gene pairs are widespread in eukaryotes, alternative processing of antisense RNAs may represent a significant form of gene regulation.

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

1990 ◽  
Vol 48 (3) ◽  
pp. 718-719
Author(s):  
Dai Dalin ◽  
Guo Jianmin
Keyword(s):  
Electron Microscope ◽  
Polyethylene Glycol ◽  
Chemical Reaction ◽  
Traditional Method ◽  
Osmium Tetroxide ◽  
Unsaturated Lipid ◽  
Embedding Method ◽  
Long Period ◽  
New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

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