scholarly journals Characterization of Intersubunit Communication in the Virginiamycin trans-Acyl Transferase Polyketide Synthase

2016 ◽  
Vol 138 (12) ◽  
pp. 4155-4167 ◽  
Author(s):  
Jonathan Dorival ◽  
Thibault Annaval ◽  
Fanny Risser ◽  
Sabrina Collin ◽  
Pierre Roblin ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Acyl Transferase ◽  
Intersubunit Communication

A polyketide synthase gene required for ochratoxin A biosynthesis in Aspergillus ochraceus

Microbiology ◽  
2003 ◽  
Vol 149 (12) ◽  
pp. 3485-3491 ◽  
Author(s):  
J. O'Callaghan ◽  
M. X. Caddick ◽  
A. D. W. Dobson
Keyword(s):  
Ochratoxin A ◽  
Polyketide Synthase ◽  
Subtractive Hybridization ◽  
Polyketide Synthases ◽  
Aspergillus Ochraceus ◽  
Predicted Amino Acid Sequence ◽  
Acyl Transferase ◽  
Reverse Transcription Pcr ◽  
Polyketide Synthase Gene

Ochratoxin A is an important nephrotoxic and nephrocarcinogenic mycotoxin, produced by Aspergillus ochraceus as a polyketide-derived secondary metabolite. A portion of a putative polyketide synthase gene (pks) involved in the biosynthesis of this mycotoxin was cloned by using a suppression subtractive hybridization PCR-based approach. The predicted amino acid sequence of the 1·4 kb clone shared 28–35 % identity to acyl transferase regions from fungal polyketide synthases found in the databases. Based on reverse transcription PCR studies, the pks gene is expressed only under ochratoxin A permissive conditions and only during the early stages of the mycotoxin synthesis. A mutant in which the pks gene has been interrupted cannot synthesize ochratoxin A. This report is the first of the cloning and characterization of a gene involved in ochratoxin A biosynthesis.

Identification and characterization of the polyketide synthase involved in ochratoxin A biosynthesis in Aspergillus carbonarius

2014 ◽  
Vol 179 ◽  
pp. 10-17 ◽  
Author(s):  
Antonia Gallo ◽  
Benjamin P. Knox ◽  
Kenneth S. Bruno ◽  
Michele Solfrizzo ◽  
Scott E. Baker ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Polyketide Synthase ◽  
Aspergillus Carbonarius ◽  
Identification And Characterization

Acyl Transferase Domains of Putative Polyketide Synthase (PKS) Genes in Aspergillus and Penicillium Producers of Ochratoxin A and the Evaluation of PCR Primers to Amplify PKS Sequences in Black Aspergillus Species

2009 ◽  
Vol 15 (1) ◽  
pp. 97-105 ◽  
Author(s):  
P.V. Martínez Culebras ◽  
A. Crespo-Sempere ◽  
J.V. Gil ◽  
D. Ramón
Keyword(s):  
Secondary Metabolites ◽  
Ochratoxin A ◽  
Polyketide Synthase ◽  
Pcr Primers ◽  
Polyketide Synthases ◽  
Aspergillus Species ◽  
Acyl Transferase ◽  
General Applicability ◽  
Phylogenetic Methods ◽  
Mycotoxin Biosynthesis

Fungal polyketide synthases (PKS) are responsible for the biosynthesis of several mycotoxins and other secondary metabolites. PKS genes in ochratoxin producing species from Aspergillus and Penicillum genera have been identified using a degenerate primer pair developed for the acyl transferase (AT) domain of fungal PKSs. Sequences of AT domains were aligned and analyzed using phylogenetic methods. The AT domain sequences appeared to be specific for a particular type of fungal PKSs and were related to PKSs involved in different mycotoxin biosynthesis pathways, including ochratoxin A. We have also developed primers suitable for amplifying AT domain sequences in strains belonging to the A. niger aggregate. DNA from most of the black Aspergillus species currently recognized was tested. Primers showed general applicability and other Aspergillus species belonging to section Nigri were successfully amplified.

scholarly journals Biochemical characterization of the minimal domains of an iterative eukaryotic polyketide synthase

FEBS Journal ◽  
2018 ◽  
Vol 285 (23) ◽  
pp. 4494-4511 ◽  
Author(s):  
Martin Sabatini ◽  
Santiago Comba ◽  
Silvia Altabe ◽  
Alejandro I. Recio‐Balsells ◽  
Guillermo R. Labadie ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Biochemical Characterization

Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin

Gene ◽  
1996 ◽  
Vol 172 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Felipe Lombó ◽  
Gloria Blanco ◽  
Ernestina Fernández ◽  
Carmen Méndez ◽  
JoséA. Salas
Keyword(s):  
Polyketide Synthase ◽  
Genes Encoding

scholarly journals A New Substrate Specificity for Acyl Transferase Domains of the Ascomycin Polyketide Synthase inStreptomyces hygroscopicus

2002 ◽  
Vol 277 (11) ◽  
pp. 9155-9159 ◽  
Author(s):  
Christopher D. Reeves ◽  
Loleta M. Chung ◽  
Yaoquan Liu ◽  
Qun Xue ◽  
John R. Carney ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Polyketide Synthase ◽  
Acyl Transferase

Functional characterization of a veA-dependent polyketide synthase gene in Aspergillus flavus necessary for the synthesis of asparasone, a sclerotium-specific pigment

2014 ◽  
Vol 64 ◽  
pp. 25-35 ◽  
Author(s):  
Jeffrey W. Cary ◽  
Pamela Y. Harris-Coward ◽  
Kenneth C. Ehrlich ◽  
José Diana Di Mavungu ◽  
Svetlana V. Malysheva ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Polyketide Synthase ◽  
Functional Characterization ◽  
Polyketide Synthase Gene

scholarly journals Genetic Localization and Molecular Characterization of the nonS Gene Required for Macrotetrolide Biosynthesis inStreptomyces griseus DSM40695

2000 ◽  
Vol 44 (7) ◽  
pp. 1809-1817 ◽  
Author(s):  
Wyatt C. Smith ◽  
Longkuan Xiang ◽  
Ben Shen
Keyword(s):  
Polyketide Synthase ◽  
Isotope Labeling ◽  
Streptomyces Griseus ◽  
Open Reading Frames ◽  
A Cell ◽  
Cyclic Polyethers ◽  
The Difference ◽  
Reading Frames

ABSTRACT The macrotetrolides are a family of cyclic polyethers derived from tetramerization, in a stereospecific fashion, of the enantiomeric nonactic acid (NA) and its homologs. Isotope labeling experiments established that NA is of polyketide origin, and biochemical investigations demonstrated that 2-methyl-6,8-dihydroxynon-2E-enoic acid can be converted into NA by a cell-free preparation from Streptomyces lividans that expresses nonS. These results lead to the hypothesis that macrotetrolide biosynthesis involves a pair of enantiospecific polyketide pathways. In this work, a 55-kb contiguous DNA region was cloned from Streptomyces griseus DSM40695, a 6.3-kb fragment of which was sequenced to reveal five open reading frames, including the previously reported nonR andnonS genes. Inactivation of nonS in vivo completely abolished macrotetrolide production. Complementation of thenonS mutant by the expression of nonS intrans fully restored its macrotetrolide production ability, with a distribution of individual macrotetrolides similar to that for the wild-type producer. In contrast, fermentation of thenonS mutant in the presence of exogenous (±)-NA resulted in the production of nonactin, monactin, and dinactin but not in the production of trinactin and tetranactin. These results prove the direct involvement of nonS in macrotetrolide biosynthesis. The difference in macrotetrolide production between in vivo complementation of the nonS mutant by the plasmid-borne nonSgene and fermentation of the nonS mutant in the presence of exogenously added (±)-NA suggests that NonS catalyzes the formation of (−)-NA and its homologs, supporting the existence of a pair of enantiospecific polyketide pathways for macrotetrolide biosynthesis inS. griseus. The latter should provide a model that can be used to study the mechanism by which polyketide synthase controls stereochemistry during polyketide biosynthesis.

Isolation and characterization of a non-reducing polyketide synthase gene in Cladonia macilenta

Mycoscience ◽  
2015 ◽  
Vol 56 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Min-Hye Jeong ◽  
Jung A Kim ◽  
Nan Hee Yu ◽  
Jae Sung Jung ◽  
Soon Gyu Hong ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Isolation And Characterization ◽  
Polyketide Synthase Gene
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