scholarly journals Live-Cell Labeling of Specific Protein Glycoforms by Proximity-Enhanced Bioorthogonal Ligation

2015 ◽  
Vol 137 (33) ◽  
pp. 10452-10455 ◽  
Author(s):  
Peter V. Robinson ◽  
Gabriela de Almeida-Escobedo ◽  
Amber E. de Groot ◽  
Julia L. McKechnie ◽  
Carolyn R. Bertozzi
Keyword(s):  
Specific Protein ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling ◽  
Protein Glycoforms

scholarly journals A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag

2019 ◽  
Author(s):  
Inbar Segal ◽  
Dikla Nachmias ◽  
Eyal Arbely ◽  
Natalie Elia
Keyword(s):  
Mammalian Cells ◽  
Fluorescent Dyes ◽  
Specific Protein ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cells ◽  
Bioorthogonal Labeling ◽  
Labeling Approach ◽  
Genetic Code Expansion ◽  
Live Cell Labeling

AbstractIn the high-resolution microscopy era, genetic code expansion (GCE)-based bioorthogonal labeling offers an elegant way for direct labeling of proteins in live cells with fluorescent dyes. This labeling approach is currently not broadly used live cell applications, partly because it needs to be adjusted to the specific protein under study. Here, we present a generic, 14-residues long, N-terminal tag for GCE-based labeling of proteins in live mammalian cells. Using this tag, we generated a library of GCE-based organelle markers, demonstrating the applicability of the tag for labeling a plethora of proteins and organelles. Finally, we show that the HA epitope, used as a backbone in our tag, can be substituted with other epitopes and, in some cases, can be completely removed, reducing the tag length to 5 residues. The GCE-tag presented here offers a powerful, easy-to-implement tool for live cell labeling of cellular proteins with small and bright probes.

Frontispiece: Highly Stabletrans-Cyclooctene Amino Acids for Live-Cell Labeling

2015 ◽  
Vol 21 (35) ◽  
pp. n/a-n/a
Author(s):  
Jan-Erik Hoffmann ◽  
Tilman Plass ◽  
Ivana Nikić ◽  
Iker Valle Aramburu ◽  
Christine Koehler ◽  
...  
Keyword(s):  
Amino Acids ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

scholarly journals Tetrazine-Containing Amino Acid for Peptide Modification and Live Cell Labeling

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0141918 ◽  
Author(s):  
Zhongqiu Ni ◽  
Lanxia Zhou ◽  
Xu Li ◽  
Jing Zhang ◽  
Shouliang Dong
Keyword(s):  
Amino Acid ◽  
Live Cell ◽  
Cell Labeling ◽  
Peptide Modification ◽  
Live Cell Labeling

scholarly journals A zeolite-based ship-in-a-bottle route to ultrasmall carbon dots for live cell labeling and bioimaging

2020 ◽  
Vol 2 (12) ◽  
pp. 5803-5809
Author(s):  
Lei Dong ◽  
Dehong Hu ◽  
Yanding Wang ◽  
Zonghai Sheng ◽  
Mei Hong ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Live Cell ◽  
Cell Labeling ◽  
Excellent Performance ◽  
Live Cell Labeling

The smallest-pore SAPO-20 zeolite confined pyrolysis of organics afforded ultrasmall uniform carbon dots with excellent performance in bioimaging.

Intracellular Delivery of Quantum Dots for Live Cell Labeling and Organelle Tracking

2004 ◽  
Vol 16 (12) ◽  
pp. 961-966 ◽  
Author(s):  
A. M. Derfus ◽  
W. C. W. Chan ◽  
S. N. Bhatia
Keyword(s):  
Quantum Dots ◽  
Intracellular Delivery ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

scholarly journals Development of SNAP-tag-mediated live cell labeling as an alternative to GFP inPorphyromonas gingivalis

2010 ◽  
Vol 59 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Ophélie Nicolle ◽  
Astrid Rouillon ◽  
Helene Guyodo ◽  
Zohreh Tamanai-Shacoori ◽  
Fatiha Chandad ◽  
...  
Keyword(s):  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

scholarly journals Live Cell Labeling with Terpyridine Derivative Proligands to Measure Cytotoxicity Mediated by Immune Cells

ChemMedChem ◽  
2017 ◽  
Vol 12 (23) ◽  
pp. 2006-2013 ◽  
Author(s):  
Yuki Sakai ◽  
Satoshi Mizuta ◽  
Asuka Kumagai ◽  
Mohammed S. O. Tagod ◽  
Hiroaki Senju ◽  
...  
Keyword(s):  
Immune Cells ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

Live Cell Labeling of Glial Progenitor Cells Using Targeted Quantum Dots

2009 ◽  
Vol 37 (10) ◽  
pp. 1967-1973 ◽  
Author(s):  
Nidhi Sabharwal ◽  
Eric C. Holland ◽  
Maribel Vazquez
Keyword(s):  
Quantum Dots ◽  
Progenitor Cells ◽  
Live Cell ◽  
Cell Labeling ◽  
Glial Progenitor Cells ◽  
Live Cell Labeling ◽  
Glial Progenitor
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