Live-Cell Protein Modification by Boronate-Assisted Hydroxamic Acid Catalysis

2021 ◽  
Vol 143 (37) ◽  
pp. 14976-14980
Author(s):  
Christopher Adamson ◽  
Hidetoshi Kajino ◽  
Shigehiro A. Kawashima ◽  
Kenzo Yamatsugu ◽  
Motomu Kanai
Keyword(s):  
Hydroxamic Acid ◽  
Protein Modification ◽  
Acid Catalysis ◽  
Live Cell ◽  
Cell Protein

scholarly journals Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins

2018 ◽  
Vol 19 (12) ◽  
pp. 3778 ◽  
Author(s):  
Nina Bozhanova ◽  
Mikhail Baranov ◽  
Nadezhda Baleeva ◽  
Alexey Gavrikov ◽  
Alexander Mishin
Keyword(s):  
Green Fluorescent Protein ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Protein Labeling ◽  
Cell Protein ◽  
Gfp Chromophore ◽  
Green Fluorescent ◽  
Derivatives Of

Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs.

Live‐Cell Protein Sulfonylation Based on Proximity‐driven N ‐Sulfonyl Pyridone Chemistry

2018 ◽  
Vol 57 (3) ◽  
pp. 659-662 ◽  
Author(s):  
Kazuya Matsuo ◽  
Yuki Nishikawa ◽  
Marie Masuda ◽  
Itaru Hamachi
Keyword(s):  
Live Cell ◽  
Cell Protein

Live‐Cell Protein Sulfonylation Based on Proximity‐driven N ‐Sulfonyl Pyridone Chemistry

2017 ◽  
Vol 130 (3) ◽  
pp. 667-670 ◽  
Author(s):  
Kazuya Matsuo ◽  
Yuki Nishikawa ◽  
Marie Masuda ◽  
Itaru Hamachi
Keyword(s):  
Live Cell ◽  
Cell Protein

Abstract LB-062: A live-cell, protein-protein interaction assay identified inhibitors of KRAS4b-G12D interaction with full-length RAF1

2020 ◽  
Author(s):  
John Columbus ◽  
Thomas J. Turbyville ◽  
Vanessa Wall ◽  
Dominic Esposito ◽  
David A. Barda ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Live Cell ◽  
Full Length ◽  
Cell Protein ◽  
Protein Protein Interaction

Profiling and Validation of Live‐Cell Protein Methylation with Engineered Enzymes and Methionine Analogues

2021 ◽  
Vol 1 (8) ◽  
Author(s):  
Nicole Weiss ◽  
Chamara Seneviranthe ◽  
Ming Jiang ◽  
Ke Wang ◽  
Minkui Luo
Keyword(s):  
Live Cell ◽  
Cell Protein ◽  
Protein Methylation

scholarly journals Live-cell protein labelling with nanometre precision by cell squeezing

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Alina Kollmannsperger ◽  
Armon Sharei ◽  
Anika Raulf ◽  
Mike Heilemann ◽  
Robert Langer ◽  
...  
Keyword(s):  
Live Cell ◽  
Cell Protein ◽  
Protein Labelling

scholarly journals Distance-Matched Tagging Sequence Optimizes Live-Cell Protein Labeling by a Biarsenical Fluorescent Reagent AsCy3_E

ACS Omega ◽  
2018 ◽  
Vol 3 (2) ◽  
pp. 2104-2110 ◽  
Author(s):  
Karen A. Hecht ◽  
Yijia Xiong ◽  
Daniel A. Barrack ◽  
Nicole R. Ford ◽  
Guritno Roesijadi ◽  
...  
Keyword(s):  
Live Cell ◽  
Protein Labeling ◽  
Cell Protein

scholarly journals A genetically encoded fluorescent tRNA is active in live-cell protein synthesis

2016 ◽  
Vol 45 (7) ◽  
pp. 4081-4093 ◽  
Author(s):  
Isao Masuda ◽  
Takao Igarashi ◽  
Reiko Sakaguchi ◽  
Ram G. Nitharwal ◽  
Ryuichi Takase ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Live Cell ◽  
Cell Protein

scholarly journals Live Cell Imaging of Enzymatic Turnover of an Adenosine 5′-Tetraphosphate Analog

2021 ◽  
Vol 22 (16) ◽  
pp. 8616
Author(s):  
Anayat Bhat ◽  
Shuang Li ◽  
Daniel Hammler ◽  
Martin J. Winterhalder ◽  
Andreas Marx ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Protein Modification ◽  
Atp Hydrolysis ◽  
Live Cell ◽  
Live Cells ◽  
Autophagic Flux ◽  
Fluorescence Sensors ◽  
Cellular Processes ◽  
Confocal Fluorescence ◽  
Hydrolysis Of

The hydrolysis of nucleotides is of paramount importance as an energy source for cellular processes. In addition, the transfer of phosphates from nucleotides onto proteins is important as a post-translational protein modification. Monitoring the enzymatic turnover of nucleotides therefore offers great potential as a tool to follow enzymatic activity. While a number of fluorescence sensors are known, so far, there are no methods available for the real-time monitoring of ATP hydrolysis inside live cells. We present the synthesis and application of a novel fluorogenic adenosine 5′-tetraphosphate (Ap4) analog suited for this task. Upon enzymatic hydrolysis, the molecule displays an increase in fluorescence intensity, which provides a readout of its turnover. We demonstrate how this can be used for monitoring cellular processes involving Ap4 hydrolysis. To this end, we visualized the enzymatic activity in live cells using confocal fluorescence microscopy of the Ap4 analog. Our results demonstrate that the Ap4 analog is hydrolyzed in lysosomes. We show that this approach is suited to visualize the lysosome distribution profiles within the live cell and discuss how it can be employed to gather information regarding autophagic flux.

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