Cell Surface Engineering Using DNAzymes: Metal Ion Mediated Control of Cell–Cell Interactions

2021 ◽  
Author(s):  
Ruo-Can Qian ◽  
Ze-Rui Zhou ◽  
Weijie Guo ◽  
Yuting Wu ◽  
Zhenglin Yang ◽  
...  
Keyword(s):  
Cell Surface ◽  
Metal Ion ◽  
Surface Engineering ◽  
Cell Interactions ◽  
Cell Surface Engineering ◽  
Cell Cell

scholarly journals Patterning of Growth Factor Gradients in Embryoid Bodies via Extracellular Matrix-Assisted Glycocalyx Engineering

2020 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
honigfort Daniel J. ◽  
purcell Sean C. ◽  
Kamil Godula
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Growth Factor ◽  
Cell Surface ◽  
Surface Engineering ◽  
Embryoid Bodies ◽  
Cell Surface Engineering

Cell surface engineering with synthetic glycomimetic co-receptors for FGF2 was used to establish gradients of stem cells with enhanced FGF2 affinity in embryoid bodies (EBs). Gradient shape was controlled by pre-assembly of glycomimetics into nanoscale vesicles with tunable dimensions and EB penetrance. <br>

scholarly journals cag+Helicobacter pylori Induces Homotypic Aggregation of Macrophage-Like Cells by Up-Regulation and Recruitment of Intracellular Adhesion Molecule 1 to the Cell Surface

2002 ◽  
Vol 70 (8) ◽  
pp. 4687-4691 ◽  
Author(s):  
Stefan Moese ◽  
Matthias Selbach ◽  
Thomas F. Meyer ◽  
Steffen Backert
Keyword(s):  
Helicobacter Pylori ◽  
Cell Surface ◽  
Adhesion Molecule ◽  
Inflammatory Responses ◽  
Cell Interactions ◽  
Lymphocyte Function ◽  
Intracellular Adhesion Molecule ◽  
Infected Cells ◽  
Cell Cell

ABSTRACT Infection with cag + but not cag-negative Helicobacter pylori leads to the formation of large homotypic aggregates of macrophage-like cells. Intracellular adhesion molecule 1 is up-regulated and recruited to the cell surface of infected cells and mediates the aggregation via lymphocyte function-associated molecule 1. This signaling may regulate cell-cell interactions and inflammatory responses.

Chemical and Enzymatic Strategies for Bacterial and Mammalian Cell Surface Engineering

2018 ◽  
Vol 24 (32) ◽  
pp. 8042-8050 ◽  
Author(s):  
Xiaobao Bi ◽  
Juan Yin ◽  
Ashley Chen Guanbang ◽  
Chuan-Fa Liu
Keyword(s):  
Cell Surface ◽  
Mammalian Cell ◽  
Surface Engineering ◽  
Cell Surface Engineering

Cell Adhesion

2004 ◽  
Author(s):  
W. Mark Saltzman
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Cell Adhesion ◽  
Cell Surface ◽  
Extracellular Space ◽  
Cell Aggregation ◽  
Solid Substrate ◽  
Cell Interactions ◽  
Phospholipid Bilayer ◽  
Cell Cell

The external surface of the cell consists of a phospholipid bilayer which carries a carbohydrate-rich coat called the glycocalyx; ionizable groups within the glycocalyx, such as sialic acid (N-acetyl neuraminate), contribute a net negative charge to the cell surface. Many of the carbohydrates that form the glycocalyx are bound to membrane-associated proteins. Each of these components— phospholipid bilayer, carbohydrate-rich coat, membrane-associated protein—has distinct physicochemical characteristics and is abundant. Plasma membranes contain ∼50% protein, ∼45% lipid, and ∼5% carbohydrate by weight. Therefore, each component influences cell interactions with the external environment in important ways. Cells can become attached to surfaces. The surface of interest may be geometrically complex (for example, the surface of another cell, a virus, a fiber, or an irregular object), but this chapter will focus on adhesion between a cell and a planar surface. The consequences of cell–cell adhesion are considered further in Chapter 8 (Cell Aggregation and Tissue Equivalents) and Chapter 9 (Tissue Barriers to Molecular and Cellular Transport). The consequences of cell–substrate adhesion are considered further in Chapter 7 (Cell Migration) and Chapter 12 (Cell Interactions with Polymers). Since the growth and function of many tissue-derived cells required attachment and spreading on a solid substrate, the events surrounding cell adhesion are fundamentally important. In addition, the strength of cell adhesion is an important determinant of the rate of cell migration, the kinetics of cell–cell aggregation, and the magnitude of tissue barriers to cell and molecule transport. Cell adhesion is therefore a major consideration in the development of methods and materials for cell delivery, tissue engineering, and tissue regeneration. The most stable and versatile mechanism for cell adhesion involves the specific association of cell surface glycoproteins, called receptors, and complementary molecules in the extracellular space, called ligands. Ligands may exist freely in the extracellular space, they may be associated with the extracellular matrix, or they may be attached to the surface of another cell. Cell–cell adhesion can occur by homophilic binding of identical receptors on different cells, by heterophilic binding of a receptor to a ligand expressed on the surface of a different cell, or by association of two receptors with an intermediate linker. Cell–matrix adhesion usually occurs by heterophilic binding of a receptor to a ligand attached to an insoluble element of the extracellular matrix.

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