Quantification of Multivalent Interactions between Sialic Acid and Influenza A Virus Spike Proteins by Single-Molecule Force Spectroscopy

2020 ◽  
Vol 142 (28) ◽  
pp. 12181-12192 ◽  
Author(s):  
Jose Luis Cuellar-Camacho ◽  
Sumati Bhatia ◽  
Valentin Reiter-Scherer ◽  
Daniel Lauster ◽  
Susanne Liese ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Influenza A Virus ◽  
Single Molecule ◽  
Influenza A ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Multivalent Interactions ◽  
Spike Proteins

scholarly journals Single Molecule Force Spectroscopy of Siloxanes

2021 ◽  
Vol 714 (3) ◽  
pp. 032023
Author(s):  
Ling Chen ◽  
Liya Yang ◽  
Chunxia Wang ◽  
Ting Zhu
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

scholarly journals Potent sialic acid inhibitors that target influenza A virus hemagglutinin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yu-Jen Chang ◽  
Cheng-Yun Yeh ◽  
Ju-Chien Cheng ◽  
Yu-Qi Huang ◽  
Kai-Cheng Hsu ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Antiviral Activity ◽  
Binding Site ◽  
Influenza A Virus ◽  
Receptor Binding ◽  
Influenza A ◽  
The United States ◽  
Ligand Complex ◽  
Receptor Binding Site ◽  
Computational Strategy

AbstractEradicating influenza A virus (IAV) is difficult, due to its genetic drift and reassortment ability. As the infectious cycle is initiated by the influenza glycoprotein, hemagglutinin (HA), which mediates the binding of virions to terminal sialic acids moieties, HA is a tempting target of anti-influenza inhibitors. However, the complexity of the HA structure has prevented delineation of the structural characterization of the HA protein–ligand complex. Our computational strategy efficiently analyzed > 200,000 records of compounds held in the United States National Cancer Institute (NCI) database and identified potential HA inhibitors, by modeling the sialic acid (SA) receptor binding site (RBS) for the HA structure. Our modeling revealed that compound NSC85561 showed significant antiviral activity against the IAV H1N1 strain with EC50 values ranging from 2.31 to 2.53 µM and negligible cytotoxicity (CC50 > 700 µM). Using the NSC85561 compound as the template to generate 12 derivatives, robust bioassay results revealed the strongest antiviral efficacies with NSC47715 and NSC7223. Virtual screening clearly identified three SA receptor binding site inhibitors that were successfully validated in experimental data. Thus, our computational strategy has identified SA receptor binding site inhibitors against HA that show IAV-associated antiviral activity.

Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy

2010 ◽  
Vol 132 (32) ◽  
pp. 11036-11038 ◽  
Author(s):  
Ningning Liu ◽  
Bo Peng ◽  
Yuan Lin ◽  
Zhaohui Su ◽  
Zhongwei Niu ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

The Nature of the Force-Induced Conformation Transition of dsDNA Studied by Using Single Molecule Force Spectroscopy

Langmuir ◽  
2010 ◽  
Vol 26 (12) ◽  
pp. 9491-9496 ◽  
Author(s):  
Ningning Liu ◽  
Tianjia Bu ◽  
Yu Song ◽  
Wei Zhang ◽  
Jinjing Li ◽  
...  
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Conformation Transition

Peptide Desorption Kinetics from Single Molecule Force Spectroscopy Studies

2014 ◽  
Vol 136 (2) ◽  
pp. 688-697 ◽  
Author(s):  
Stefanie Krysiak ◽  
Susanne Liese ◽  
Roland R. Netz ◽  
Thorsten Hugel
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Desorption Kinetics ◽  
Single Molecule Force Spectroscopy ◽  
Spectroscopy Studies

scholarly journals Quantifying DNA melting transitions using single-molecule force spectroscopy

2008 ◽  
Vol 21 (3) ◽  
pp. 034114 ◽  
Author(s):  
Christopher P Calderon ◽  
Wei-Hung Chen ◽  
Kuan-Jiuh Lin ◽  
Nolan C Harris ◽  
Ching-Hwa Kiang
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Dna Melting ◽  
Single Molecule Force Spectroscopy ◽  
Melting Transitions
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