scholarly journals Correction to “Inverted Binding of Non-natural Substrates in Strictosidine Synthase Leads to a Switch of Stereochemical Outcome in Enzyme-Catalyzed Pictet–Spengler Reactions”

2020 ◽  
Vol 142 (9) ◽  
pp. 4525-4526 ◽  
Author(s):  
Elisabeth Eger ◽  
Adam Simon ◽  
Mahima Sharma ◽  
Song Yang ◽  
Willem B. Breukelaar ◽  
...  
Keyword(s):  
Strictosidine Synthase

scholarly journals Nucleotide sequence of a cDNA encoding the vacuolar protein strictosidine synthase fromCatharanthus roseus

1990 ◽  
Vol 18 (16) ◽  
pp. 4939-4939 ◽  
Author(s):  
T.D. McKnight ◽  
C.A. Roessner ◽  
R. Devagupta ◽  
A.I. Scott ◽  
C.L. Nessler
Keyword(s):  
Nucleotide Sequence ◽  
Strictosidine Synthase ◽  
Vacuolar Protein

scholarly journals Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus

Planta ◽  
1998 ◽  
Vol 205 (3) ◽  
pp. 414-419 ◽  
Author(s):  
Camilo Canel ◽  
M. Inês Lopes-Cardoso ◽  
Serap Whitmer ◽  
Leslie van der Fits ◽  
Giancarlo Pasquali ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Catharanthus Roseus ◽  
Tryptophan Decarboxylase ◽  
Alkaloid Production ◽  
Strictosidine Synthase ◽  
Over Expression

Phylogenetic analysis and functional characterisation of strictosidine synthase-like genes in Arabidopsis thaliana

2009 ◽  
Vol 36 (12) ◽  
pp. 1098 ◽  
Author(s):  
Natalie A. J. Kibble ◽  
M. Mehdi Sohani ◽  
Neil Shirley ◽  
Caitlin Byrt ◽  
Ute Roessner ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Phylogenetic Analysis ◽  
Plant Protection ◽  
Pcr Analysis ◽  
Strictosidine Synthase ◽  
Metabolomics Data ◽  
Unknown Compound ◽  
Class A ◽  
Functional Characterisation ◽  
Arabidopsis Protein

Monoterpenoid indole alkaloids (MIA) are a diverse class of secondary metabolites important for plant protection and are drugs for treating human diseases. Arabidopsis thaliana (L.) is not known to produce MIAs, yet its genome has 15 genes with similarity to the periwinkle (Catharanthus roseus (L.) G. Don) strictosidine synthase (STR) gene. Phylogenetic analysis of strictosidine synthase-like (SSL) proteins reveals four well supported classes of SSLs in Arabidopsis. To determine if Arabidopsis produces active strictosidine synthase, Arabidopsis protein extracts were assayed for enzymatic activity and cDNAs were expressed in Escherichia coli. Arabidopsis protein extracts from leaves and hairy roots do not make strictosidine at levels comparable to C. roseus, but they metabolise one substrate, secologanin, a precursor of strictosidine in other plant species, and produce an ‘unknown’ compound proposed to be a dimer of secologanic acid. Recombinant Arabidopsis proteins expressed in E. coli were not active STRs. Quantitative PCR analysis was performed on class A Ssls and showed they are upregulated by salt, ultraviolet light and salicylic acid treatment. RNAi mutants of Arabidopsis with reduced expression of all four class A Ssls, suggest that class A SSL proteins can modify secologanin. Gene expression and metabolomics data suggests that class A Ssl genes may have a role in plant protection.

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