scholarly journals Automated Backbone NMR Resonance Assignment of Large Proteins Using Redundant Linking from a Single Simultaneous Acquisition

2020 ◽  
Vol 142 (12) ◽  
pp. 5793-5799 ◽  
Author(s):  
Jan Stanek ◽  
Tobias Schubeis ◽  
Piotr Paluch ◽  
Peter Güntert ◽  
Loren B. Andreas ◽  
...  
Keyword(s):  
Resonance Assignment ◽  
Nmr Resonance Assignment ◽  
Large Proteins ◽  
Simultaneous Acquisition

Assigning large proteins in the solid state: a MAS NMR resonance assignment strategy using selectively and extensively 13C-labelled proteins

2009 ◽  
Vol 44 (4) ◽  
pp. 245-260 ◽  
Author(s):  
Victoria A. Higman ◽  
Jeremy Flinders ◽  
Matthias Hiller ◽  
Stefan Jehle ◽  
Stefan Markovic ◽  
...  
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Nmr Resonance Assignment ◽  
Mas Nmr ◽  
Large Proteins ◽  
Assignment Strategy

scholarly journals Erratum to: NMR resonance assignment of DnaE intein from Nostoc punctiforme

2012 ◽  
Vol 7 (1) ◽  
pp. 115-116
Author(s):  
Kimmo Heinämäki ◽  
Jesper S. Oeemig ◽  
Kimmo Pääkkönen ◽  
Janica Djupsjöbacka ◽  
Hideo Iwaï
Keyword(s):  
Resonance Assignment ◽  
Nmr Resonance Assignment ◽  
Nostoc Punctiforme

scholarly journals Mixed pyruvate labeling enables backbone resonance assignment of large proteins using a single experiment

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Scott A. Robson ◽  
Koh Takeuchi ◽  
Andras Boeszoermenyi ◽  
Paul W. Coote ◽  
Abhinav Dubey ◽  
...  
Keyword(s):  
Resonance Assignment ◽  
Backbone Resonance Assignment ◽  
Single Experiment ◽  
Large Proteins ◽  
Backbone Resonance

scholarly journals NMR resonance assignment and dynamics of profilin from Heimdallarchaeota

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Syed Razaul Haq ◽  
Sabeen Survery ◽  
Fredrik Hurtig ◽  
Ann-Christin Lindås ◽  
Celestine N. Chi
Keyword(s):  
Structural Analysis ◽  
Structural Properties ◽  
Resonance Assignment ◽  
Eukaryotic Cell ◽  
Nmr Resonance Assignment ◽  
Metagenomic Data ◽  
Divergent Evolution ◽  
Structural Elements ◽  
Scientific Question ◽  
Secondary Structural Elements

Abstract The origin of the eukaryotic cell is an unsettled scientific question. The Asgard superphylum has emerged as a compelling target for studying eukaryogenesis due to the previously unseen diversity of eukaryotic signature proteins. However, our knowledge about these proteins is still relegated to metagenomic data and very little is known about their structural properties. Additionally, it is still unclear if these proteins are functionally homologous to their eukaryotic counterparts. Here, we expressed, purified and structurally characterized profilin from Heimdallarchaeota in the Asgard superphylum. The structural analysis shows that while this profilin possesses similar secondary structural elements as eukaryotic profilin, it contains additional secondary structural elements that could be critical for its function and an indication of divergent evolution.

NMR resonance assignment of DnaE intein from Nostoc punctiforme

2009 ◽  
Vol 3 (1) ◽  
pp. 41-43 ◽  
Author(s):  
Kimmo Heinämäki ◽  
Jesper S. Oeemig ◽  
Janica Djupsjöbacka ◽  
Hideo Iwaï
Keyword(s):  
Resonance Assignment ◽  
Nmr Resonance Assignment ◽  
Nostoc Punctiforme
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