Loop-Directed Mutagenesis of the Blue Copper Protein Amicyanin fromParacoccus versutusand Its Effect on the Structure and the Activity of the Type-1 Copper Site

2000 ◽  
Vol 122 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Christian Buning ◽  
Gerard W. Canters ◽  
Peter Comba ◽  
Christopher Dennison ◽  
Lars Jeuken ◽  
...  
Keyword(s):  
Directed Mutagenesis ◽  
Blue Copper Protein ◽  
Blue Copper ◽  
Copper Protein ◽  
Copper Site ◽  
Type 1 Copper

ChemInform Abstract: Kinetic, Spectroscopic, and Structural (Extended X-Ray Absorption Fine Structure) Studies on the Type 1 Blue Copper Protein Umecyanin.

ChemInform ◽  
1987 ◽  
Vol 18 (9) ◽  
Author(s):  
S. K. CHAPMAN ◽  
W. H. ORME-JOHNSON ◽  
J. MCGINNIS ◽  
J. D. SINCLAIR-DAY ◽  
A. G. SYKES ◽  
...  
Keyword(s):  
Fine Structure ◽  
Blue Copper Protein ◽  
Blue Copper ◽  
Copper Protein ◽  
X Ray ◽  
Absorption Fine ◽  
X Ray Absorption

scholarly journals An e.p.r. study of the non-equivalence of the copper sites of caeruloplasmin

1986 ◽  
Vol 238 (1) ◽  
pp. 291-295 ◽  
Author(s):  
L Calabrese ◽  
M Carbonaro
Keyword(s):  
Slow Phase ◽  
Copper Binding ◽  
Blue Copper ◽  
Sequence Of Events ◽  
Copper Site ◽  
Hyperfine Splitting Constant ◽  
Type 1 Copper ◽  
Splitting Constant

The two Type 1 (blue) copper-binding sites of caeruloplasmin were spectroscopically differentiated by the kinetic analysis of the e.p.r. spectra during the redox cycle. One blue copper, with a hyperfine splitting constant (A parallel) of 6.8 mT, which was rapidly reduced, was not reoxidized by oxygen, whereas it was reoxidized by H2O2. The other blue copper (A parallel = 5.8 mT), which was reduced slowly, was rapidly reoxidized by either oxygen or H2O2. A conformational change of the Type 2 copper was concomitant with the fast reduction of Type 1 copper, whereas its reduction occurred during the slow phase. This sequence of events was reversed in the reoxidation step, that is, the Type 2 copper reappeared rapidly as the species with altered conformation and reverted to the symmetry typical of the native state in the slow phase. The specific reaction of a blue-copper site with the H2O2 can tentatively be related to the established ability of caeruloplasmin to prevent ‘oxidative’ attack of proteins and lipids.

Type 1 blue copper protein amicyanin from Thiobacillus versutus: line-broadening effects of chromium(III) complexes and related studies

1995 ◽  
pp. 3395 ◽  
Author(s):  
Christopher Dennison ◽  
Panayotis Kyritsis ◽  
Arnout P. Kalverda ◽  
Gerard W. Canters ◽  
William McFarlane ◽  
...  
Keyword(s):  
Blue Copper Protein ◽  
Line Broadening ◽  
Blue Copper ◽  
Copper Protein ◽  
Thiobacillus Versutus

scholarly journals Modulating the copper–sulfur interaction in type 1 blue copper azurin by replacing Cys112 with nonproteinogenic homocysteine

2014 ◽  
Vol 1 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Kevin M. Clark ◽  
Yang Yu ◽  
Wilfred A. van der Donk ◽  
Ninian J. Blackburn ◽  
Yi Lu
Keyword(s):  
Amino Acid ◽  
Blue Copper ◽  
Copper Site ◽  
Type 1 Copper

Replacement of conserved Cys112 in azurin with a nonproteinogenic amino acid homocysteine affords a type-1 copper site with decreased Cu–SCys covalency.

scholarly journals Loop-Directed Mutagenesis Converts Amicyanin fromThiobacillus versutusinto a Novel Blue Copper Protein

1996 ◽  
Vol 118 (31) ◽  
pp. 7406-7407 ◽  
Author(s):  
Christopher Dennison ◽  
Erik Vijgenboom ◽  
Wilfred R. Hagen ◽  
Gerard W. Canters
Keyword(s):  
Directed Mutagenesis ◽  
Blue Copper Protein ◽  
Blue Copper ◽  
Copper Protein

Solution Structure of the Type 1 Blue Copper Protein Amicyanin from Thiobacillus versutus

1994 ◽  
Vol 240 (4) ◽  
pp. 358-371 ◽  
Author(s):  
Arnout P. Kalverda ◽  
Sybren S. Wymenga ◽  
Arjen Lommen ◽  
Frank J.M. van de Ven ◽  
Cees W. Hilbers ◽  
...  
Keyword(s):  
Solution Structure ◽  
Blue Copper Protein ◽  
Blue Copper ◽  
Copper Protein ◽  
Thiobacillus Versutus

Kinetic, spectroscopic, and structural (extended X-ray absorption fine structure) studies on the type 1 blue copper protein umecyanin

1986 ◽  
pp. 2063 ◽  
Author(s):  
Stephen K. Chapman ◽  
William H. Orme-Johnson ◽  
Joseph McGinnis ◽  
John D. Sinclair-Day ◽  
A. Geoffrey Sykes ◽  
...  
Keyword(s):  
Fine Structure ◽  
Blue Copper Protein ◽  
Blue Copper ◽  
Copper Protein ◽  
X Ray ◽  
Absorption Fine ◽  
X Ray Absorption

Redox reactivity of the type 1 (blue) copper protein amicyanin from Thiobacillus versutus with inorganic complexes

1994 ◽  
pp. 3017 ◽  
Author(s):  
Panayotis Kyritsis ◽  
Christopher Dennison ◽  
Arnout P. Kalverda ◽  
Gerard W. Canters ◽  
A. Geoffrey Sykes
Keyword(s):  
Blue Copper Protein ◽  
Blue Copper ◽  
Copper Protein ◽  
Thiobacillus Versutus ◽  
Redox Reactivity ◽  
Inorganic Complexes
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