Polarization-Modulated FT-IR Spectroscopy Studies of Acetylcholinesterase Secondary Structure at the Air−Water Interface

1999 ◽  
Vol 121 (41) ◽  
pp. 9618-9625 ◽  
Author(s):  
Leila Dziri ◽  
Bernard Desbat ◽  
Roger M. Leblanc
Keyword(s):  
Secondary Structure ◽  
Ir Spectroscopy ◽  
Water Interface ◽  
Air Water Interface ◽  
Ft Ir ◽  
Spectroscopy Studies ◽  
Ft Ir Spectroscopy

Polarization-Modulated FT-IR Spectroscopy of a Spread Monolayer at the Air/Water Interface

1993 ◽  
Vol 47 (7) ◽  
pp. 869-874 ◽  
Author(s):  
D. Blaudez ◽  
T. Buffeteau ◽  
J. C. Cornut ◽  
B. Desbat ◽  
N. Escafre ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Liquid Water ◽  
Arachidic Acid ◽  
Water Molecules ◽  
Water Interface ◽  
Vibrational Modes ◽  
Experimental Conditions ◽  
Air Water Interface ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

This study devoted to the FT-IR spectroscopy of monolayers spread at the air/water interface is, to our knowledge, the first report presenting complete mid-infrared monolayer spectra perfectly extracted from the strong water vapor bands. This has been possible with the use of the polarization-modulated IRRAS method, which is not sensitive to the isotropic absorptions of the sample environment. On the basis of theoretical modeling and experiments, the best angle of incidence has been found near 76° for detection of intraplane as well as out-of-plane oriented monolayer absorptions. With the use of such experimental conditions, on the normalized difference (covered vs. uncovered water) PM-IRRAS spectra, monolayer vibrational bands come out upward or downward, depending on the orientation of their transition moment with respect to the interface. Application to the study of deuterated arachidic acid and arachidate monolayers allows observation of the vibrational modes of the polar head groups interacting with the liquid water molecules and provides some evidence of their symmetrical anchoring. The vibrational modes of the liquid water subphase contribute to these difference spectra as broad dips that certainly contain information on a possible restructuring of the water molecules at the interface.

FT-IR spectroscopy studies of the breast cancer cell composition changes induced by Au-BSA nanoclusters

2016 ◽  
Vol 231 ◽  
pp. S93 ◽  
Author(s):  
Mara Grube ◽  
Karlis Shvirksts ◽  
Dominyka Dapkute ◽  
Marija Matulionyte ◽  
Elina Zandberga ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ir Spectroscopy ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cell Composition ◽  
Ft Ir ◽  
Spectroscopy Studies ◽  
Composition Changes ◽  
Ft Ir Spectroscopy
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