Chimeric Triterpene Synthase. A Possible Model for Multifunctional Triterpene Synthase

1999 ◽  
Vol 121 (6) ◽  
pp. 1208-1216 ◽  
Author(s):  
Tetsuo Kushiro ◽  
Masaaki Shibuya ◽  
Yutaka Ebizuka
Keyword(s):  
Triterpene Synthase

Functional genomics approach to the study of triterpene biosynthesis

2003 ◽  
Vol 75 (2-3) ◽  
pp. 369-374 ◽  
Author(s):  
Yutaka Ebizuka ◽  
Yuji Katsube ◽  
T. Tsutsumi ◽  
Tetsuo Kushiro ◽  
M. Shibuya
Keyword(s):  
Primary Metabolism ◽  
Liquid Chromatography Mass Spectrometry ◽  
Reaction Products ◽  
Water Addition ◽  
Sequencing Project ◽  
Oxidosqualene Cyclase ◽  
Cycloartenol Synthase ◽  
Polymerase Chain ◽  
Triterpene Synthase ◽  
Triterpene Synthases

The Arabidopsis thaliana genome-sequencing project has identified the presence of 13 oxidosqualene cyclase homologs in this plant. In addition to the already identified clones, namely, CAS1 cycloartenol synthase, LUP1 lupeol synthase, and YUP8H12R.43 multifunctional triterpene synthase, two new cDNAs of the putative oxidosqualene cyclase genes, F1019.4 and T30F21.16, were obtained by polymerase chain reaction (PCR) and functionally expressed in yeast. Liquid chromatography/mass spectrometry (LC/MS) analysis led to the identification of some of their reaction products. Interestingly, except for CAS1 for sterol biosynthesis of primary metabolism, so-far-obtained all triterpene synthases of this plant are multifunctional, producing more than one cyclization product. A feeding experiment of 13C-labeled acetate with LUP1 lupeol synthase transformant demonstrated the stereospecific water addition to lupenyl cation intermediate, yielding 3β,20 dihydroxylupane, which accounts for the multiproduct nature of this synthase.

scholarly journals Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica , involved in biosynthesis of bryonolic acid

2001 ◽  
Vol 268 (23) ◽  
pp. 6311-6317 ◽  
Author(s):  
Hiroaki Hayashi ◽  
Pengyu Huang ◽  
Kenichiro Inoue ◽  
Noboru Hiraoka ◽  
Yasumasa Ikeshiro ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Luffa Cylindrica ◽  
Triterpene Synthase

scholarly journals Construction of an artificial system for ambrein biosynthesis and investigation of some biological activities of ambrein

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yota Yamabe ◽  
Yukina Kawagoe ◽  
Kotone Okuno ◽  
Mao Inoue ◽  
Kanako Chikaoka ◽  
...  
Keyword(s):  
Biological Activities ◽  
Osteoclast Differentiation ◽  
Amyloid Β ◽  
Sperm Whale ◽  
Efficient Production ◽  
Artificial System ◽  
Bacterial Enzyme ◽  
Odor Compounds ◽  
Triterpene Synthase ◽  
Induced Apoptosis

AbstractAmbergris, a sperm whale metabolite, has long been used as a fragrance and traditional medication, but it is now rarely available. The odor components of ambergris result from the photooxidative degradation of the major component, ambrein. The pharmacological activities of ambergris have also been attributed to ambrein. However, efficient production of ambrein and odor compounds has not been achieved. Here, we constructed a system for the synthesis of ambrein and odor components. First, we created a new triterpene synthase, “ambrein synthase,” for mass production of ambrein by redesigning a bacterial enzyme. The ambrein yields were approximately 20 times greater than those reported previously. Next, an efficient photooxidative conversion system from ambrein to a range of volatiles of ambergris was established. The yield of volatiles was 8–15%. Finally, two biological activities, promotion of osteoclast differentiation and prevention of amyloid β-induced apoptosis, were discovered using the synthesized ambrein.

Cyclization of All-E- and 2Z-Geranylfarnesols by a Bacterial Triterpene Synthase: Insight into Sesterterpene Biosynthesis inAleuritopterisFerns

2013 ◽  
Vol 77 (11) ◽  
pp. 2278-2282 ◽  
Author(s):  
Junichi SHINOZAKI ◽  
Masaaki SHIBUYA ◽  
Yutaka EBIZUKA ◽  
Kazuo MASUDA
Keyword(s):  
Triterpene Synthase ◽  
Insight Into

scholarly journals Protein modelling of triterpene synthase genes from mangrove plants using Phyre2 and Swiss-model

2018 ◽  
Vol 978 ◽  
pp. 012095 ◽  
Author(s):  
M Basyuni ◽  
R Wati ◽  
N Sulistiyono ◽  
R Hayati ◽  
Sumardi ◽  
...  
Keyword(s):  
Protein Modelling ◽  
Mangrove Plants ◽  
Triterpene Synthase

scholarly journals An unusual plant triterpene synthase with predominant α-amyrin-producing activity identified by characterizing oxidosqualene cyclases from Malus × domestica

FEBS Journal ◽  
2011 ◽  
Vol 278 (14) ◽  
pp. 2485-2499 ◽  
Author(s):  
Cyril Brendolise ◽  
Yar-Khing Yauk ◽  
Ellen D. Eberhard ◽  
Mindy Wang ◽  
David Chagne ◽  
...  
Keyword(s):  
Malus Domestica ◽  
Oxidosqualene Cyclases ◽  
Triterpene Synthase

A New Triterpene Synthase fromArabidopsisthalianaProduces a Tricyclic Triterpene with Two Hydroxyl Groups

2006 ◽  
Vol 8 (13) ◽  
pp. 2835-2838 ◽  
Author(s):  
Ting Xiang ◽  
Masaaki Shibuya ◽  
Yuji Katsube ◽  
Takehiko Tsutsumi ◽  
Miyuki Otsuka ◽  
...  
Keyword(s):  
Hydroxyl Groups ◽  
Triterpene Synthase

A novel multifunctional triterpene synthase from Arabidopsis thaliana

2000 ◽  
Vol 41 (40) ◽  
pp. 7705-7710 ◽  
Author(s):  
Tetsuo Kushiro ◽  
Masaaki Shibuya ◽  
Kazuo Masuda ◽  
Yutaka Ebizuka
Keyword(s):  
Arabidopsis Thaliana ◽  
Triterpene Synthase

Identification of Novel Sesterterpene/Triterpene Synthase fromBacillus clausii

ChemBioChem ◽  
2013 ◽  
Vol 14 (7) ◽  
pp. 822-825 ◽  
Author(s):  
Tsutomu Sato ◽  
Hiroaki Yamaga ◽  
Shoji Kashima ◽  
Yusuke Murata ◽  
Tetsuro Shinada ◽  
...  
Keyword(s):  
Triterpene Synthase

Molecular cloning and functional expression of a multifunctional triterpene synthase cDNA from a mangrove species Kandelia candel (L.) Druce

2006 ◽  
Vol 67 (23) ◽  
pp. 2517-2524 ◽  
Author(s):  
Mohammad Basyuni ◽  
Hirosuke Oku ◽  
Masashi Inafuku ◽  
Shigeyuki Baba ◽  
Hironori Iwasaki ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Expression ◽  
Kandelia Candel ◽  
Mangrove Species ◽  
Triterpene Synthase
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