Novel Peptide Nucleic Acid That Shows High Sequence Specificity and All-or-None-Type Hybridization with the Complementary DNA

1999 ◽  
Vol 121 (1) ◽  
pp. 256-257 ◽  
Author(s):  
Masayasu Kuwahara ◽  
Miki Arimitsu ◽  
Masahiko Sisido
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Sequence Specificity ◽  
Complementary Dna

scholarly journals Reliable microspotting methodology for peptide-nucleic acid layers with high hybridization efficiency on gold SPR imaging chips

2015 ◽  
Vol 7 (15) ◽  
pp. 6077-6082 ◽  
Author(s):  
L. Simon ◽  
G. Lautner ◽  
R. E. Gyurcsányi
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gold Surfaces ◽  
Complementary Dna ◽  
Spr Imaging ◽  
Hybridization Efficiency ◽  
Dna Strand

Microspotting of HS-PNA strands prehybridized with a complementary DNA strand onto gold surfaces results in PNA layers with excellent hybridization efficiency.

PNA–sugar conjugates as tools for the spatial screening of carbohydrate–lectin interactions

2011 ◽  
Vol 84 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Christian Scheibe ◽  
Oliver Seitz
Keyword(s):  
Nucleic Acid ◽  
Protein Interactions ◽  
Peptide Nucleic Acid ◽  
Modular Approach ◽  
Complementary Dna ◽  
Double Helices ◽  
Biological Recognition ◽  
Dna Strands ◽  
Carbohydrate Ligands ◽  
Spatial Presentation

Multivalent carbohydrate–lectin interactions are essential for a multitude of biological recognition events. Much effort has been spent in the synthesis of potent multivalent scaffolds in order to mimic or inhibit biological carbohydrate–protein interactions. However, the defined spatial presentation of carbohydrates remained a challenging task. Peptide nucleic acid (PNA)- and DNA-based double helices are useful scaffolds that enable the controlled display of carbohydrate ligands in a modular approach. The hybridization of PNA-sugar conjugates with complementary DNA strands provides multivalent complexes with defined spatial presentation of carbohydrates, which facilitates the spatial screening of carbohydrate–lectin interactions with Ångström-scale precision.

Quenching of fluorescently labeled pyrrolidinyl peptide nucleic acid by oligodeoxyguanosine and its application in DNA sensing

2020 ◽  
Vol 18 (30) ◽  
pp. 5951-5962
Author(s):  
Chayan Charoenpakdee ◽  
Tirayut Vilaivan
Keyword(s):  
Nucleic Acid ◽  
Electrostatic Interaction ◽  
Peptide Nucleic Acid ◽  
Dna Sensing ◽  
Complementary Dna

Oligodeoxyguanosine effectively quenches the fluorescence of PNA probes via electrostatic interaction, and the signal is restored by the addition of complementary DNA targets.

Pyrene-labeled pyrrolidinyl peptide nucleic acid as a hybridization-responsive DNA probe: comparison between internal and terminal labeling

RSC Advances ◽  
2014 ◽  
Vol 4 (17) ◽  
pp. 8817-8827 ◽  
Author(s):  
Chalothorn Boonlua ◽  
Boonsong Ditmangklo ◽  
Nisanath Reenabthue ◽  
Chaturong Suparpprom ◽  
Nattawee Poomsuk ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Dna Probe ◽  
Complementary Dna

Internally pyrene-labeled pyrrolidinyl PNA yields much larger fluorescence increase than terminally labeled PNA upon hybridization with complementary DNA.

Downregulation of TdT Expression through Splicing Modulation by Antisense Peptide Nucleic Acid (PNA)

2019 ◽  
Vol 20 (2) ◽  
pp. 168-178 ◽  
Author(s):  
Soheila Montazersaheb ◽  
Masoumeh Kazemi ◽  
Elahe Nabat ◽  
Peter E. Nielsen ◽  
Mohammad S. Hejazi
Keyword(s):  
Nucleic Acid ◽  
Cell Survival ◽  
Peptide Nucleic Acid ◽  
Lymphoblastic Leukemia ◽  
Pcr Analysis ◽  
Sequence Specificity ◽  
Apoptosis Induction ◽  
Base Mismatch ◽  
Splicing Patterns ◽  
Antisense Peptide

Background and Objective: Antisense oligonucleotides are able to modulate splicing patterns and offer therapeutic intervention for cancer and other diseases. Considering TdT potential as a target in cancer therapy, the present study aimed to investigate splicing alteration of TdT pre-mRNA in Molt-4 cells using peptide nucleic acid (PNA) octaarginine and cholic acid conjugates. Method: We examined 16 mer PNAs targeting 5' and 3' junctions of intron 7 and addressed their mRNA splicing modulation effects using RT-PCR analysis. We also tested corresponding 2-base mismatch PNAs to confirm the sequence specificity. In addition, protien level of TdT, apoptosis induction and cell viability rate were analysed. Results: PCR analysis showed that full match PNAs could modulate the splicing process, thereby producing a longer mRNA still including intron 7. PCR results also implied exon 7 skipping. In addition, reduced level of TdT protein in Molt-4 cells was observed. Downregulation of TdT level in PNA treated cells was accompanied by an increased rate of apoptosis and decreased the level of cell survival. Conclusion: PNA-mediated splicing modulation can specifically downregulate TdT expression. TdT dowregulation results in apoptosis induction and reduced cell survival in Molt-4 cells. These observations could draw more attentions to develop PNA based strategies for TdT suppression and consequent apoptosis induction in acute lymphoblastic leukemia.

scholarly journals Electrofluidic Positioning of Biofunctionalized Nanowires

2008 ◽  
Vol 1144 ◽  
Author(s):  
Thomas J. Morrow ◽  
Jaekyun Kim ◽  
Mingwei Li ◽  
Theresa S. Mayer ◽  
Christine D. Keating
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Imaging ◽  
Peptide Nucleic Acid ◽  
Chip Surface ◽  
Complementary Dna ◽  
The Individual

ABSTRACTWe functionalized nanowires with three different probe peptide nucleic acid (PNA) sequences, and assembled the three populations onto a lithographically patterned chip. Electrofluidic assembly enabled positioning each set of nanowires to span a different pair of guiding electrodes. Fluorescence imaging was used to probe whether the PNA on the individual nanowires remained able to selectively bind complementary DNA targets following assembly and integration of the positioned nanowires onto the chip surface.

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

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