Circular Dichroism and UV−Visible Absorption Spectra of the Langmuir−Blodgett Films of an Aggregating Helicene

1998 ◽  
Vol 120 (34) ◽  
pp. 8656-8660 ◽  
Author(s):  
Colin Nuckolls ◽  
Thomas J. Katz ◽  
Thierry Verbiest ◽  
Sven Van Elshocht ◽  
Hans-Georg Kuball ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Absorption Spectra ◽  
Visible Absorption ◽  
Langmuir Blodgett ◽  
Langmuir Blodgett Films ◽  
Uv Visible ◽  
Circular Dichroïsm

Effects of Two Trypsin Inhibitors on Trypsin in Activity and Structure

2014 ◽  
Vol 1073-1076 ◽  
pp. 1824-1827
Author(s):  
Shu Ting Dong ◽  
Hong Zhang ◽  
Na Xu ◽  
Ping Li ◽  
Si Si Xu ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Trypsin Inhibitor ◽  
Cd Spectroscopy ◽  
Trypsin Inhibitors ◽  
Kunitz Trypsin Inhibitor ◽  
Visible Absorption ◽  
Uv Visible ◽  
Circular Dichroïsm ◽  
Visible Absorption Spectroscopy ◽  
Inhibit Factor

Two reversible trypsin inhibitors, Kunitz trypsin inhibitor (KTI) and Bowman-Birk trypsin inhibitor (BBI) were compared to find the more optimal one as the inhibit factor during trypsin immobilization. Fluorescence spectroscopy, UV–visible absorption spectroscopy and circular dichroism (CD) spectroscopy were used to explore the effects of the two inhibitors on trypsin in activity and structure. The results showed that both inhibitors combined with trypsin in 1:1. CD circular dichroism spectroscopy showed that KTI and BBI led to different changes in trypsin second structure. The results can help us find out the mechanism between the two inhibitors and trypsin and select the more optimal inhibitor in trypsin immobilization.

scholarly journals ComparativeIn VitroBinding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Pamita Awasthi ◽  
Nitesh Kumar ◽  
Raj Kaushal ◽  
Mohan Kumar ◽  
Shrikant Kukreti
Keyword(s):  
Circular Dichroism ◽  
Binding Constants ◽  
Blue Shift ◽  
Binding Mode ◽  
Visible Absorption ◽  
Calf Thymus ◽  
Absorption Studies ◽  
Uv Visible ◽  
Ct Dna ◽  
Circular Dichroïsm

The binding of TiCl2(dpme)2(1), (dpme = 6,6′-dimethyl-2,2′-bipyridine), Ti(ada)2(bzac)2(2), (ada = adamantylamine; bzac = benzoylacetone), and TiCl2(bzac)(bpme) (3), (bpme = 4,4′-dimethyl-2,2′-bipyrdine) with calf thymus (ct) DNA has been studied by UV-visible spectroscopy, thermal denaturation, and circular dichroism spectroscopy. In UV-visible study complexes1,2, and3showed red, blue, and red shifts, respectively, upon the addition of ct-DNA along with a significant hyperchromism. The intrinsic binding constants (Kb) calculated from UV-visible absorption studies were 2.3 × 103 M−1, 3.3 × 103 M−1and, 7.1 × 103 M−1for complexes1,2, and3, respectively. The change in melting temperature (ΔTm) was calculated to be 2-3°C for each complex. Circular dichroism (CD) study showed blue shift for complex2and red shift for complexes1and3along with rise in molecular ellipticity upon the addition of complexes. Results suggest a binding mode of complex2different than1and3.

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