T Cells Recognize a Glycopeptide Derived from Type II Collagen in a Model for Rheumatoid Arthritis

1998 ◽  
Vol 120 (31) ◽  
pp. 7676-7683 ◽  
Author(s):  
Johan Broddefalk ◽  
Johan Bäcklund ◽  
Fredrik Almqvist ◽  
Martin Johansson ◽  
Rikard Holmdahl ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Type Ii Collagen ◽  
Type Ii

scholarly journals Type II collagen is a target antigen of clonally expanded T cells in the synovium of patients with rheumatoid arthritis

1999 ◽  
Vol 58 (7) ◽  
pp. 446-450 ◽  
Author(s):  
T. Sekine ◽  
T. Kato ◽  
K. Masuko-Hongo ◽  
H. Nakamura ◽  
S.-i. Yoshino ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Type Ii Collagen ◽  
Target Antigen ◽  
Type Ii

Effector function of type II collagen-stimulated T cells from rheumatoid arthritis patients: Cross-talk between T cells and synovial fibroblasts

2004 ◽  
Vol 50 (3) ◽  
pp. 776-784 ◽  
Author(s):  
Mi-La Cho ◽  
Chong-Hyeon Yoon ◽  
Sue-Yun Hwang ◽  
Mi-Kyung Park ◽  
So-Youn Min ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Cross Talk ◽  
Synovial Fibroblasts ◽  
Type Ii Collagen ◽  
Effector Function ◽  
Type Ii

scholarly journals Evaluation of a fit-for-purpose assay to monitor antigen-specific functional CD4+ T cell subpopulations in rheumatoid arthritis using flow-cytometry based peptide-MHC class-II tetramer staining

2021 ◽  
Author(s):  
Swati Patel ◽  
Nishta Ramnoruth ◽  
Pascale Wehr ◽  
Jamie Rossjohn ◽  
Hugh H Reid ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Flow Cytometry ◽  
T Cells ◽  
Mhc Class Ii ◽  
Cd4 T Cells ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Response Biomarker ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Abstract Antigen-specific T cells can serve as a response biomarker in non-clinical or clinical immunotherapy studies in autoimmune disease. There are protocols with optimized multimer staining methods to detect peptide (p)MHCII+ CD4+ T cells, and some qualified and validated protocols for pMHCI+ CD8+ T cells. However, no protocol is fully or partially qualified to enumerate and characterise antigen-specific pMHCII+ CD4+ T cells from patient samples. Implementing such an assay requires a desired level of specificity and precision, in terms of assay repeatability and reproducibility. In transgenic type II collagen (CII)-immunised HLA-DR1/DR4 humanised mouse models of collagen-induced arthritis (CIA), CII259-273-specific T cells dominantly expand. Therefore antigen-specific T cells recognising this epitope presented by rheumatoid arthritis (RA)-associated risk HLA-DR allomorphs are of interest to understand disease progression and responses to immunotherapy in RA patients. Using HLA-DRB1*04:01 or *01:01-collagen type II (CII)259–273 tetramers, we evaluated parameters influencing precision and reproducibility of an optimized flow cytometry-based method for antigen-specific CD4+ T cells and eight specific subpopulations with and without tetramer positivity. We evaluated specificity, precision, and reproducibility for research environments and non-regulated laboratories. The assay has excellent overall precision with %CV<25% for intra-assay repeatability, inter-analyst precision, and inter-assay reproducibility. The precision of the assay correlated negatively with the cell viability after thawing, indicating that post-thaw viability is a critical parameter for reproducibility. This assay is suitable for longitudinal analysis of treatment response and disease activity outcome in RA patients, and adaptable for translational or immunotherapy clinical trial settings.

Altered peptide ligands control type II collagen-reactive T cells from rheumatoid arthritis patients

2006 ◽  
Vol 16 (4) ◽  
pp. 226-228 ◽  
Author(s):  
Yasuyuki Ohnishi ◽  
Akito Tsutsumi ◽  
Isao Matsumoto ◽  
Daisuke Goto ◽  
Satoshi Ito ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Type Ii Collagen ◽  
Peptide Ligands ◽  
Type Ii ◽  
Altered Peptide Ligands

scholarly journals Shift toward T helper 1 cytokines by type II collagen-reactive T cells in patients with rheumatoid arthritis

2001 ◽  
Vol 44 (3) ◽  
pp. 561-569 ◽  
Author(s):  
Sung-Hwan Park ◽  
Do-June Min ◽  
Mi-La Cho ◽  
Wan-Uk Kim ◽  
Jeehee Youn ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Type Ii Collagen ◽  
Type Ii ◽  
T Helper ◽  
T Helper 1
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