scholarly journals Raman and Infrared Spectroscopy of Cyanide-Inhibited CO Dehydrogenase/Acetyl-CoA Synthase fromClostridium thermoaceticum:  Evidence for Bimetallic Enzymatic CO Oxidation [J.Am.Chem.Soc.1996,118, 10429−10435].

1997 ◽  
Vol 119 (45) ◽  
pp. 11134-11135 ◽  
Author(s):  
Di Qiu ◽  
Manoj Kumar ◽  
Stephen W. Ragsdale ◽  
Thomas G. Spiro
Keyword(s):  
Infrared Spectroscopy ◽  
Co Oxidation ◽  
Co Dehydrogenase ◽  
Acetyl Coa ◽  
Raman And Infrared Spectroscopy

scholarly journals Heterologous Expression of the Clostridium carboxidivorans CO Dehydrogenase Alone or Together with the Acetyl Coenzyme A Synthase Enables both Reduction of CO2 and Oxidation of CO by Clostridium acetobutylicum

2017 ◽  
Vol 83 (16) ◽  
Author(s):  
Ellinor D. Carlson ◽  
Eleftherios T. Papoutsakis
Keyword(s):  
Co Oxidation ◽  
Clostridium Acetobutylicum ◽  
Coenzyme A ◽  
Functional Expression ◽  
Co Dehydrogenase ◽  
Acetyl Coa ◽  
Acetyl Coenzyme A ◽  
Content Type ◽  
Acetyl Coenzyme ◽  
Clostridium Carboxidivorans

ABSTRACT With recent advances in synthetic biology, CO2 could be utilized as a carbon feedstock by native or engineered organisms, assuming the availability of electrons. Two key enzymes used in autotrophic CO2 fixation are the CO dehydrogenase (CODH) and acetyl coenzyme A (acetyl-CoA) synthase (ACS), which form a bifunctional heterotetrameric complex. The CODH/ACS complex can reversibly catalyze CO2 to CO, effectively enabling a biological water-gas shift reaction at ambient temperatures and pressures. The CODH/ACS complex is part of the Wood-Ljungdahl pathway (WLP) used by acetogens to fix CO2, and it has been well characterized in native hosts. So far, only a few recombinant CODH/ACS complexes have been expressed in heterologous hosts, none of which demonstrated in vivo CO2 reduction. Here, functional expression of the Clostridium carboxidivorans CODH/ACS complex is demonstrated in the solventogen Clostridium acetobutylicum, which was engineered to express CODH alone or together with the ACS. Both strains exhibited CO2 reduction and CO oxidation activities. The CODH reactions were interrogated using isotopic labeling, thus verifying that CO was a direct product of CO2 reduction, and vice versa. CODH apparently uses a native C. acetobutylicum ferredoxin as an electron carrier for CO2 reduction. Heterologous CODH activity depended on actively growing cells and required the addition of nickel, which is inserted into CODH without the need to express the native Ni insertase protein. Increasing CO concentrations in the gas phase inhibited CODH activity and altered the metabolite profile of the CODH-expressing cells. This work provides the foundation for engineering a complete and functional WLP in nonnative host organisms. IMPORTANCE Functional expression of CO dehydrogenase (CODH) from Clostridium carboxidivorans was demonstrated in C. acetobutylicum, which is natively incapable of CO2 fixation. The expression of CODH, alone or together with the C. carboxidivorans acetyl-CoA synthase (ACS), enabled C. acetobutylicum to catalyze both CO2 reduction and CO oxidation. Importantly, CODH exhibited activity in both the presence and absence of ACS. 13C-tracer studies confirmed that the engineered C. acetobutylicum strains can reduce CO2 to CO and oxidize CO during growth on glucose.

Biochemical differences in tumorigenic and nontumorigenic cells measured by Raman and infrared spectroscopy

2005 ◽  
Vol 10 (3) ◽  
pp. 031106 ◽  
Author(s):  
Judith R. Mourant ◽  
Kurt W. Short ◽  
Susan Carpenter ◽  
Nagapratima Kunapareddy ◽  
Leslie Coburn ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Raman And Infrared Spectroscopy

Moganite detection in silica rocks using Raman and infrared spectroscopy

2014 ◽  
Vol 25 (5) ◽  
pp. 797-805 ◽  
Author(s):  
Patrick Schmidt ◽  
Ludovic Bellot-Gurlet ◽  
Vanessa Leá ◽  
Philippe Sciau
Keyword(s):  
Infrared Spectroscopy ◽  
Raman And Infrared Spectroscopy ◽  
Silica Rocks
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