Biopolymer Conformational Distributions from Solid-State NMR:  α-Helix and 310-Helix Contents of a Helical Peptide

1998 ◽  
Vol 120 (28) ◽  
pp. 7039-7048 ◽  
Author(s):  
Henry W. Long ◽  
Robert Tycko
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Helical Peptide ◽  
Α Helix ◽  
310 Helix

scholarly journals Controlling 310-Helix and α-Helix of Short Peptides in the Solid State

2007 ◽  
Vol 55 (5) ◽  
pp. 840-842 ◽  
Author(s):  
Yosuke Demizu ◽  
Masakazu Tanaka ◽  
Masanobu Nagano ◽  
Masaaki Kurihara ◽  
Mitsunobu Doi ◽  
...  
Keyword(s):  
Solid State ◽  
Short Peptides ◽  
Α Helix ◽  
310 Helix

scholarly journals pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy

2018 ◽  
Vol 115 (48) ◽  
pp. 12194-12199 ◽  
Author(s):  
Sarah A. Otieno ◽  
Samuel Z. Hanz ◽  
Bianca Chakravorty ◽  
Anqi Zhang ◽  
Lukas M. Klees ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Membrane Surface ◽  
Multistage Model ◽  
Solid State Nmr Spectroscopy ◽  
Ph Values ◽  
Lipid Dynamics ◽  
Ph Range ◽  
Α Helix

The applications of the pH low insertion peptide (pHLIP) in cancer diagnosis and cross-membrane cargo delivery have drawn increasing attention in the past decade. With its origin as the transmembrane (TM) helix C of bacteriorhodopsin, pHLIP is also an important model for understanding how pH can affect the folding and topogenesis of a TM α-helix. Protonations of multiple D/E residues transform pHLIP from an unstructured coil at membrane surface (known as state II, at pH ≥ 7) to a TM α-helix (state III, pH ≤ 5.3). While these initial and end states of pHLIP insertion have been firmly established, what happens at the intervening pH values is less clear. However, the intervening pH range is most relevant to pHLIP−cell interactions in the acidic extracellular tumor environment (and in the endosomes within cells). Here, using advanced solid-state NMR spectroscopy with palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine unilamellar vesicles as the model membrane, we systematically examined the state of pHLIP−membrane interactions (in terms of the membrane locations of D/E residues, as well as lipid dynamics) at the intervening pH values of 6.4, 6.1, and 5.8, along with the known states at pH 7.4 and 5.3. Thermodynamic intermediate states distinct from the initial and end states were discovered to exist at each of the intervening pH examined. They support a multistage model of pHLIP insertion in which the D/E titrations occur in a defined sequence at distinct intermediate pH values. This multistage model has important ramifications in pHLIP applications.

Determination of an 8-.ANG. interatomic distance in a helical peptide by solid-state NMR spectroscopy

1992 ◽  
Vol 114 (12) ◽  
pp. 4830-4833 ◽  
Author(s):  
Susan M. Holl ◽  
Garland R. Marshall ◽  
Denise D. Beusen ◽  
Karol Kociolek ◽  
Adam S. Redlinski ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Interatomic Distance ◽  
Solid State Nmr Spectroscopy ◽  
Helical Peptide

A useful tool for the elaboration of new solid-state NMR experiments: PULSAR

1995 ◽  
Vol 92 ◽  
pp. 1939-1942 ◽  
Author(s):  
JP Amoureux ◽  
C Fernandez ◽  
Y Dumazy
Keyword(s):  
Solid State ◽  
Solid State Nmr

Quantitative analysis by 13C solid-state NMR of eumelanins

1994 ◽  
Vol 91 ◽  
pp. 881-887 ◽  
Author(s):  
M Hervé ◽  
J Hirschinger ◽  
P Granger ◽  
P Gilard ◽  
N Goetz
Keyword(s):  
Quantitative Analysis ◽  
Solid State ◽  
Solid State Nmr
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