Bispheric Coordinative Structuring in a Zinc Finger Protein:  NMR Analysis of a Point Mutant of the Carboxy-Terminal LIM Domain of Quail Cysteine- and Glycine-Rich Protein CRP2

1998 ◽  
Vol 120 (28) ◽  
pp. 7127-7128 ◽  
Author(s):  
Robert Konrat ◽  
Ralf Weiskirchen ◽  
Klaus Bister ◽  
Bernhard Kräutler
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Protein Nmr ◽  
Nmr Analysis ◽  
Lim Domain ◽  
Point Mutant ◽  
Finger Protein ◽  
Carboxy Terminal ◽  
Glycine Rich Protein

Opl: a zinc finger protein that regulates neural determination and patterning in Xenopus

Development ◽  
1998 ◽  
Vol 125 (15) ◽  
pp. 2867-2882 ◽  
Author(s):  
J.S. Kuo ◽  
M. Patel ◽  
J. Gamse ◽  
C. Merzdorf ◽  
X. Liu ◽  
...  
Keyword(s):  
Neural Crest ◽  
Zinc Finger ◽  
Neural Tube ◽  
Zinc Finger Protein ◽  
Neural Patterning ◽  
Dorsal Neural Tube ◽  
Finger Protein ◽  
Pair Rule Gene ◽  
Carboxy Terminal ◽  
Pair Rule

In order to study the mechanism of neural patterning in Xenopus, we used subtractive cloning to isolate genes activated early during this process. One gene isolated was opl, (odd-paired-like) that resembles the Drosophila pair-rule gene odd-paired and encodes a zinc finger protein that is a member of the Zic gene family. At the onset of gastrulation, opl is expressed throughout the presumptive neural plate, indicating that neural determination has begun at this stage while, by neurula, opl expression is restricted to the dorsal neural tube and neural crest. opl encodes a transcriptional activator, with a carboxy terminal regulatory domain, which when removed increases opl activity. opl both sensitizes animal cap ectoderm to the neural inducer noggin and alters the spectrum of genes induced by noggin, allowing activation of the midbrain marker engrailed. Consistent with the later dorsal neural expression of opl, the activated form of opl is able to induce neural crest and dorsal neural tube markers both in animal caps and whole embryos. In ventral ectoderm, opl induces formation of loose cell aggregates that may indicate neural crest precursor cells. Aggregates do not express an epidermal marker, indicating that opl suppresses ventral fates. Together, these data suggest that opl may mediate neural competence and may be involved in activation of midbrain, dorsal neural and neural crest fates.

Cloning and functional analysis of SCTF-1 encoding a C2H2-type zinc finger protein from soybean

2012 ◽  
Vol 34 (6) ◽  
pp. 749-756 ◽  
Author(s):  
Bing SONG ◽  
Pi-Wu WANG ◽  
Yong-Ping FU ◽  
Xu-Hong FAN ◽  
Hai-Feng XIA ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein
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