HIV-1 TAR RNA Recognition by an Unnatural Biopolymer

1997 ◽  
Vol 119 (27) ◽  
pp. 6444-6445 ◽  
Author(s):  
Xilu Wang ◽  
Ikramul Huq ◽  
Tariq M. Rana
Keyword(s):  
Rna Recognition ◽  
Tar Rna ◽  
Hiv 1

scholarly journals Structure of a low-population binding intermediate in protein-RNA recognition

2016 ◽  
Vol 113 (26) ◽  
pp. 7171-7176 ◽  
Author(s):  
Aditi N. Borkar ◽  
Michael F. Bardaro ◽  
Carlo Camilloni ◽  
Francesco A. Aprile ◽  
Gabriele Varani ◽  
...  
Keyword(s):  
Residual Dipolar Couplings ◽  
Energy Landscape ◽  
General Strategy ◽  
Rna Recognition ◽  
Widespread Occurrence ◽  
Transactivator Of Transcription ◽  
Tar Rna ◽  
Conformational Rearrangements ◽  
Nmr Residual Dipolar Couplings ◽  
Hiv 1

The interaction of the HIV-1 protein transactivator of transcription (Tat) and its cognate transactivation response element (TAR) RNA transactivates viral transcription and represents a paradigm for the widespread occurrence of conformational rearrangements in protein-RNA recognition. Although the structures of free and bound forms of TAR are well characterized, the conformations of the intermediates in the binding process are still unknown. By determining the free energy landscape of the complex using NMR residual dipolar couplings in replica-averaged metadynamics simulations, we observe two low-population intermediates. We then rationally design two mutants, one in the protein and another in the RNA, that weaken specific nonnative interactions that stabilize one of the intermediates. By using surface plasmon resonance, we show that these mutations lower the release rate of Tat, as predicted. These results identify the structure of an intermediate for RNA-protein binding and illustrate a general strategy to achieve this goal with high resolution.

scholarly journals Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR

Science ◽  
1991 ◽  
Vol 251 (5001) ◽  
pp. 1597-1600 ◽  
Author(s):  
A Gatignol ◽  
A Buckler-White ◽  
B Berkhout ◽  
K. Jeang
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Tar Rna ◽  
Hiv 1

scholarly journals Exosomes from HIV-1-infected Cells Stimulate Production of Pro-inflammatory Cytokines through Trans-activating Response (TAR) RNA

2015 ◽  
Vol 291 (3) ◽  
pp. 1251-1266 ◽  
Author(s):  
Gavin C. Sampey ◽  
Mohammed Saifuddin ◽  
Angela Schwab ◽  
Robert Barclay ◽  
Shreya Punya ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Infected Cells ◽  
Tar Rna ◽  
Hiv 1 ◽  
Pro Inflammatory Cytokines

Aromatic Polyamidines Inhibiting the Tat-Induced HIV-1 Transcription Recognize Structured TAR-RNA

2001 ◽  
Vol 11 (4) ◽  
pp. 209-217 ◽  
Author(s):  
Carlo Mischiati ◽  
Kuan-Teh Jeang ◽  
Giordana Feriotto ◽  
Laura Breda ◽  
Monica Borgatti ◽  
...  
Keyword(s):  
Tar Rna ◽  
Hiv 1
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