Determination of γ and Stereospecific Assignment of H5‘ Protons by Measurement of2Jand3JCoupling Constants in Uniformly13C Labeled RNA

1996 ◽  
Vol 118 (18) ◽  
pp. 4388-4395 ◽  
Author(s):  
J. P. Marino ◽  
H. Schwalbe ◽  
S. J. Glaser ◽  
C. Griesinger
Keyword(s):  
Stereospecific Assignment ◽  
Labeled Rna

In SituHybridization with33P-Labeled RNA Probes for Determination of Cellular Expression Patterns of Liver Transcription Factors in Mouse Embryos

Methods ◽  
1998 ◽  
Vol 16 (1) ◽  
pp. 29-41 ◽  
Author(s):  
Francisco M. Rausa ◽  
Honggang Ye ◽  
Lorena Lim ◽  
Stephen A. Duncan ◽  
Robert H. Costa
Keyword(s):  
Transcription Factors ◽  
Expression Patterns ◽  
Mouse Embryos ◽  
Cellular Expression ◽  
Rna Probes ◽  
In Situhybridization ◽  
Labeled Rna

scholarly journals DRUID: A pipeline for reproducible transcriptome-wide measurements of mRNA stability

2017 ◽  
Author(s):  
Andrew Lugowski ◽  
Beth Nicholson ◽  
Olivia S. Rissland
Keyword(s):  
Mrna Stability ◽  
Messenger Rna ◽  
Time Course ◽  
High Throughput Sequencing ◽  
Computational Algorithms ◽  
Metabolic Labeling ◽  
Computational Pipeline ◽  
Time Course Data ◽  
Labeled Rna

SUMMARYControl of messenger RNA (mRNA) stability is an important aspect of gene regulation. The gold standard for measuring mRNA stability transcriptome-wide uses metabolic labeling, biochemical isolation of labeled RNA populations, and high-throughput sequencing. However, difficult normalization procedures and complex computational algorithms have inhibited widespread adoption of this approach. Here, we present DRUID (for Determination of Rates Using Intron Dynamics), a computational pipeline that is robust, easy to use, and freely available. Our pipeline uses endogenous introns to normalize time course data and yields more reproducible half-lives than other methods, even with datasets that were otherwise unusable. DRUID can handle datasets from a variety of organisms, spanning yeast to humans, and we even applied it retroactively on published datasets. We anticipate that DRUID will allow broad application of metabolic labeling for studies of mRNA stability.

In SituHybridization with33P-Labeled RNA Probes for Determination of Cellular Expression Patterns of Liver Transcription Factors in Mouse Embryos

Methods ◽  
1998 ◽  
Vol 16 (3) ◽  
pp. 359-360 ◽  
Author(s):  
Francisco M. Rausa ◽  
Honggang Ye ◽  
Lorena Lim ◽  
Stephen A. Duncan ◽  
Robert H. Costa
Keyword(s):  
Transcription Factors ◽  
Expression Patterns ◽  
Mouse Embryos ◽  
Cellular Expression ◽  
Rna Probes ◽  
In Situhybridization ◽  
Labeled Rna

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Distance to SN 1987A and the LMC

1999 ◽  
Vol 190 ◽  
pp. 549-554
Author(s):  
Nino Panagia
Keyword(s):  
Angular Size ◽  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Light Curves ◽  
Distance Modulus ◽  
Absolute Size ◽  
Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

International determination of the total motion of the pole

1961 ◽  
Vol 13 ◽  
pp. 29-41
Author(s):  
Wm. Markowitz
Keyword(s):  
Secular Motion ◽  
The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

Time and Latitude in South Africa

1972 ◽  
Vol 1 ◽  
pp. 27-38
Author(s):  
J. Hers
Keyword(s):  
South Africa ◽  
Cape Town ◽  
Astronomical Observations ◽  
Royal Observatory ◽  
The Republic ◽  
Astronomical Determination ◽  
Limited Accuracy ◽  
Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

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