scholarly journals NMR Dynamics Investigation of Ligand-Induced Changes of Main and Side-Chain Arginine N−H’s in Human Phosphomevalonate Kinase

2010 ◽  
Vol 132 (7) ◽  
pp. 2102-2103 ◽  
Author(s):  
Andrew L. Olson ◽  
Sheng Cai ◽  
Timothy J. Herdendorf ◽  
Henry M. Miziorko ◽  
Daniel S. Sem
Keyword(s):  
Side Chain ◽  
Phosphomevalonate Kinase ◽  
Induced Changes

scholarly journals NMR dynamics investigation of ligand induced changes of main and side chain arginine N‐H's in human phosphomevalonate kinase

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Andrew L Olson ◽  
Huili Yao ◽  
Timothy Herdendorf ◽  
Henry Miziorko ◽  
Sheng Cai ◽  
...  
Keyword(s):  
Side Chain ◽  
Phosphomevalonate Kinase ◽  
Induced Changes

scholarly journals Characterization of small molecule induced changes in Parkinson’s-related trafficking via the Nedd4 ubiquitin signaling cascade

2020 ◽  
Author(s):  
A. Katherine Hatstat ◽  
Hannah D. Ahrendt ◽  
Matthew W. Foster ◽  
Leland Mayne ◽  
M. Arthur Moseley ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
Side Chain ◽  
Dependent Manner ◽  
Associated Proteins ◽  
Molecular Mechanism Of Action ◽  
Trafficking Defects ◽  
Induced Changes ◽  
Early Onset Parkinson’S Disease

SummaryThe benzdiimidazole NAB2 rescues α-synuclein-associated trafficking defects associated with early onset Parkinson’s disease in a Nedd4-dependent manner. Despite identification of E3 ubiquitin ligase Nedd4 as a putative target of NAB2, its molecular mechanism of action has not been elucidated. As such, the effect of NAB2 on Nedd4 activity and specificity was interrogated through biochemical, biophysical, and proteomic analyses. NAB2 was found to bind Nedd4 (KDapp = 42 nM), but this binding is side chain mediated and does not alter its conformation or ubiquitination kinetics in vitro. Nedd4 co-localizes with trafficking organelles, and NAB2 exposure did not alter its colocalization. Ubiquitin-enrichment coupled proteomics revealed that NAB2 stimulates ubiquitination of trafficking and transport associated proteins, most likely through modulating the substrate specificity of Nedd4, providing a putative protein network involved in the NAB2 mechanism.

scholarly journals NMR characterization of substrate induced changes in structure and dynamics of human phosphomevalonate kinase

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Andrew L Olson ◽  
Huili Yao ◽  
Shen Cai ◽  
Tim Herdendorf ◽  
Henry M Miziorko ◽  
...  
Keyword(s):  
Structure And Dynamics ◽  
Nmr Characterization ◽  
Phosphomevalonate Kinase ◽  
Induced Changes

Synchrotron X-Ray Radiation (CHESS) Study of Electric-Field Induced Changes in the Structure of Thermotropic Side-Chain Liquid Crystals

1993 ◽  
Vol 307 ◽  
Author(s):  
T. J. Bunning ◽  
S. G. McNamee ◽  
C. M. McHugh ◽  
S. S. Patnaik ◽  
C. K. Ober ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Electric Field ◽  
High Energy ◽  
Side Chain ◽  
Synchrotron Source ◽  
Correlation Lengths ◽  
Range Correlation ◽  
Induced Changes ◽  
Kinetics Of ◽  
Director Orientation

ABSTRACTSynchrotron radiation from the Cornell High Energy Synchrotron Source (CHESS) was used to probe the alignment kinetics of a class of thermotropic siloxane-based liquid crystals under the influence of an electric field. The high flux allows for real-time investigation of microstructural changes as a function of frequency and temperature. The packing behavior of a pentamethylcyclosiloxane ring with pendant biphenyl mesogens was thoroughly investigated. The director orientation parameter, Sd, typically increased with decreasing temperature and was not strongly affected by frequency. Electric-field induced changes in the director orientation were also monitored. Upon application of an electric field, the nematic system exhibited a tendency to pack in columns with short-range correlation of the mesogens. Column correlation lengths are examined as a function of temperature for two frequencies. At the low temperature end of the mesophase, a tendency for the mesogens to layer pack was observed.

scholarly journals Magnetic-field-induced changes of the isotropic-nematic phase transition in side-chain polymer liquid crystals

2003 ◽  
Vol 67 (5) ◽  
Author(s):  
M. I. Boamfǎ ◽  
K. Viertler ◽  
A. Wewerka ◽  
F. Stelzer ◽  
P. C. M. Christianen ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Phase Transition ◽  
Liquid Crystals ◽  
Nematic Phase ◽  
Side Chain ◽  
Chain Polymer ◽  
Polymer Liquid ◽  
Polymer Liquid Crystals ◽  
Induced Changes

Interpretation of irradiation-induced changes in electron diffractograms and images of extinction contours at low temperatures

1984 ◽  
Vol 42 ◽  
pp. 268-269
Author(s):  
E. Knapek ◽  
H. Formanek ◽  
G. Lefranc ◽  
I. Dietrich
Keyword(s):  
Low Temperatures ◽  
Carbon Films ◽  
Organic Crystals ◽  
Wide Spread ◽  
Lens System ◽  
Preparation Conditions ◽  
Thin Carbon ◽  
Electron Diffractograms ◽  
Diffraction Patterns ◽  
Induced Changes

A few years ago results on cryoprotection of L-valine were reported, where the values of the critical fluence De i.e, the electron exposure which decreases the intensity of the diffraction reflections by a factor e, amounted to the order of 2000 + 1000 e/nm2. In the meantime a discrepancy arose, since several groups published De values between 100 e/nm2 and 1200 e/nm2 /1 - 4/. This disagreement and particularly the wide spread of the results induced us to investigate more thoroughly the behaviour of organic crystals at very low temperatures during electron irradiation.For this purpose large L-valine crystals with homogenuous thickness were deposited on holey carbon films, thin carbon films or Au-coated holey carbon films. These specimens were cooled down to nearly liquid helium temperature in an electron microscope with a superconducting lens system and irradiated with 200 keU-electrons. The progress of radiation damage under different preparation conditions has been observed with series of electron diffraction patterns and direct images of extinction contours.

Drug Induced Changes in Cell Organelles

1968 ◽  
Vol 26 ◽  
pp. 110-111
Author(s):  
Sarah A. Luse
Keyword(s):  
Clinical Medicine ◽  
Cell Organelles ◽  
Riboflavin Deficiency ◽  
Drug Induced ◽  
New Era ◽  
Health And Disease ◽  
In The Beginning ◽  
Cellular Pathology ◽  
Induced Changes ◽  
The Impact

In the mid-nineteenth century Virchow revolutionized pathology by introduction of the concept of “cellular pathology”. Today, a century later, this term has increasing significance in health and disease. We now are in the beginning of a new era in pathology, one which might well be termed “organelle pathology” or “subcellular pathology”. The impact of lysosomal diseases on clinical medicine exemplifies this role of pathology of organelles in elucidation of disease today.Another aspect of cell organelles of prime importance is their pathologic alteration by drugs, toxins, hormones and malnutrition. The sensitivity of cell organelles to minute alterations in their environment offers an accurate evaluation of the site of action of drugs in the study of both function and toxicity. Examples of mitochondrial lesions include the effect of DDD on the adrenal cortex, riboflavin deficiency on liver cells, elevated blood ammonia on the neuron and some 8-aminoquinolines on myocardium.

Digital x-ray mapping of nanometer-size supported bimetallic catalysts

1988 ◽  
Vol 46 ◽  
pp. 530-531
Author(s):  
L. T. Germinario
Keyword(s):  
Background Radiation ◽  
Metal Cluster ◽  
Single Element ◽  
Beam Diameter ◽  
X Rays ◽  
X Ray ◽  
Major Interest ◽  
Induced Changes

Understanding the role of metal cluster composition in determining catalytic selectivity and activity is of major interest in heterogeneous catalysis. The electron microscope is well established as a powerful tool for ultrastructural and compositional characterization of support and catalyst. Because the spatial resolution of x-ray microanalysis is defined by the smallest beam diameter into which the required number of electrons can be focused, the dedicated STEM with FEG is the instrument of choice. The main sources of errors in energy dispersive x-ray analysis (EDS) are: (1) beam-induced changes in specimen composition, (2) specimen drift, (3) instrumental factors which produce background radiation, and (4) basic statistical limitations which result in the detection of a finite number of x-ray photons. Digital beam techniques have been described for supported single-element metal clusters with spatial resolutions of about 10 nm. However, the detection of spurious characteristic x-rays away from catalyst particles produced images requiring several image processing steps.

Immunogold localization of calmodulin in root caps of the maize Cultivar Merit

1990 ◽  
Vol 48 (3) ◽  
pp. 674-675
Author(s):  
P.T. Nguyen ◽  
C. Uphoff ◽  
C.L. Stinemetz
Keyword(s):  
Calcium Binding ◽  
Calcium Transport ◽  
Primary Role ◽  
Immunogold Localization ◽  
Root Tips ◽  
Electrical Currents ◽  
Considerable Evidence ◽  
Maize Cultivar ◽  
Gravity Response ◽  
Induced Changes

Considerable evidence suggest that the calcium-binding protein calmodulin (CaM) may mediate calcium action and/or transport important in the gravity response of plants. Calmodulin is present in both shoots and roots and is capable of regulating calcium transport in plant vesicles. In roots calmodulin is concentrated in the tip, the gravisensing region of the root; and is reported to be closely associated with amyloplasts, organelles suggested to play a primary role in gravi-perception. Inhibitors of CaM such as chlorpromazine, calmidazolium, and compound 48/80 interfere with the gravitropic response of both snoots and roots. The magnitude of the inhibition corresponded well with the extent to which the drug binds to endogenous CaM. Compound 48/80 and calmidazolium block gravi-induced changes in electrical currents across root tips, a phenomenon thought to be associated with the sensing of the gravity stimulus.In this study, we have investigated the subcellular distribution of CaM in graviresponsive and non-graviresponsive root caps of the maize cultivar Merit.

Identifying groups of airborne particles by ordination

1994 ◽  
Vol 52 ◽  
pp. 856-857
Author(s):  
M. Shlepr ◽  
C. M. Vicroy
Keyword(s):  
Elemental Analysis ◽  
Energy Dispersive Spectroscopy ◽  
Manufacturing Process ◽  
Spatial Representation ◽  
Airborne Particles ◽  
Common Source ◽  
Data Set ◽  
Microelectronics Industry ◽  
Induced Changes ◽  
Source Materials

The microelectronics industry is heavily tasked with minimizing contaminates at all steps of the manufacturing process. Particles are generated by physical and/or chemical fragmentation from a mothersource. The tools and macrovolumes of chemicals used for processing, the environment surrounding the process, and the circuits themselves are all potential particle sources. A first step in eliminating these contaminants is to identify their source. Elemental analysis of the particles often proves useful toward this goal, and energy dispersive spectroscopy (EDS) is a commonly used technique. However, the large variety of source materials and process induced changes in the particles often make it difficult to discern if the particles are from a common source.Ordination is commonly used in ecology to understand community relationships. This technique usespair-wise measures of similarity. Separation of the data set is based on discrimination functions. Theend product is a spatial representation of the data with the distance between points equaling the degree of dissimilarity.

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