scholarly journals Reactivity of Glass-Embedded Met Hemoglobin Derivatives toward External NO: Implications for Nitrite-Mediated Production of Bioactive NO

2009 ◽  
Vol 131 (34) ◽  
pp. 12273-12279 ◽  
Author(s):  
Mahantesh S. Navati ◽  
Joel M. Friedman
Keyword(s):  
Hemoglobin Derivatives

Methemoglobin in blood as determined by double-wavelength spectrophotometry.

1982 ◽  
Vol 28 (3) ◽  
pp. 508-511 ◽  
Author(s):  
M Sakata ◽  
A Yoshida ◽  
M Haga
Keyword(s):  
Carbon Monoxide ◽  
Spectrophotometric Determination ◽  
Potassium Ferricyanide ◽  
Hemoglobin Derivatives ◽  
Theoretical Results

Abstract We describe spectrophotometric determination of methemoglobin (Met-Hb) in blood by absorbance differences at two wavelengths (characteristic of the instrument used) in the 500-600 nm region. Blood is diluted 100- to 200-fold with a solution containing KCN and carbon monoxide to convert hemoglobin to carboxyhemoglobin and Met-Hb to cyanomethemoglobin (CN-Met-Hb). Carboxyhemoglobin has the same absorbance at the two wavelengths used, so that the differences in absorbance reflect only the CN-Met-Hb component. After measurement of absorbance differences, potassium ferricyanide is added to convert all hemoglobin derivatives to CN-Met-Hb; samples are then remeasured at the same wavelengths. The percentage of Met-Hb is determined from the ratio of the absorbance differences. Results agree satisfactorily with currently accepted procedures and with calculated theoretical results for mixtures of oxyhemoglobin, carboxyhemoglobin, and Met-Hb.

Theory of hyperfine interactions and associated properties in hemin and hemoglobin derivatives

1978 ◽  
Vol 4 (1-2) ◽  
pp. 914-920 ◽  
Author(s):  
Mahendra K. Mallick ◽  
Seong Ki Mun ◽  
Shantilata Mishra ◽  
Jane C. Chang ◽  
T. P. Das
Keyword(s):  
Hyperfine Interactions ◽  
Hemoglobin Derivatives

Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer.

1984 ◽  
Vol 30 (3) ◽  
pp. 373-379 ◽  
Author(s):  
A Zwart ◽  
A Buursma ◽  
E J van Kampen ◽  
W G Zijlstra
Keyword(s):  
Simultaneous Determination ◽  
Separate Determination ◽  
Overdetermined System ◽  
Chemical Research ◽  
Chemical Laboratory ◽  
Multicomponent Analysis ◽  
Hemoglobin Derivatives ◽  
Clinical Chemical ◽  
Human Blood Samples

Abstract We describe a method for the simultaneous determination of the five clinically relevant hemoglobin derivatives (Hb, HbO2, HbCO, Hi, SHb) in a blood sample by means of a reversed-optics spectrophotometer (Hewlett-Packard HP8450 A UV/Vis). A built-in computer program is used for multicomponent analysis in an overdetermined system, i.e., a system in which the number of independent equations used exceeds the number of unknowns to be determined. First, the spectra of the five hemoglobin derivatives are measured in a series of different human blood samples. Thereafter, the multicomponent method for the simultaneous determination of the five hemoglobin derivatives is tested by comparison with conventional methods for the separate determination of oxygen saturation, HbCO, Hi, and SHb fractions. The multicomponent (multiwavelength) method is sufficiently reliable, accurate, and easy to justify its use in physiological chemical research as well as its routine application in the clinical chemical laboratory.

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