scholarly journals Differential Dynamical Effects of Macromolecular Crowding on an Intrinsically Disordered Protein and a Globular Protein: Implications for In-Cell NMR Spectroscopy

2008 ◽  
Vol 130 (20) ◽  
pp. 6310-6311 ◽  
Author(s):  
Conggang Li ◽  
Lisa M. Charlton ◽  
Asha Lakkavaram ◽  
Christopher Seagle ◽  
Guifang Wang ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Macromolecular Crowding ◽  
Intrinsically Disordered Protein ◽  
Globular Protein ◽  
Intrinsically Disordered ◽  
Disordered Protein

scholarly journals Intrinsically Disordered Protein Exhibits Both Compaction and Expansion under Macromolecular Crowding

2018 ◽  
Vol 114 (5) ◽  
pp. 1067-1079 ◽  
Author(s):  
Anthony Banks ◽  
Sanbo Qin ◽  
Kevin L. Weiss ◽  
Christopher B. Stanley ◽  
Huan-Xiang Zhou
Keyword(s):  
Macromolecular Crowding ◽  
Intrinsically Disordered Protein ◽  
Intrinsically Disordered ◽  
Disordered Protein

Self-Assembling Micelles Based on an Intrinsically Disordered Protein Domain

2018 ◽  
Author(s):  
Sarah Klass ◽  
Matthew J. Smith ◽  
Tahoe Fiala ◽  
Jessica Lee ◽  
Anthony Omole ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Fusion Proteins ◽  
Organic Molecules ◽  
Intrinsically Disordered Protein ◽  
Protein Domain ◽  
Enzymatic Cleavage ◽  
Intrinsically Disordered ◽  
Disordered Protein ◽  
Self Assembling ◽  
Protein Tag

Herein, we describe a new series of fusion proteins that have been developed to self-assemble spontaneously into stable micelles that are 27 nm in diameter after enzymatic cleavage of a solubilizing protein tag. The sequences of the proteins are based on a human intrinsically disordered protein, which has been appended with a hydrophobic segment. The micelles were found to form across a broad range of pH, ionic strength, and temperature conditions, with critical micelle concentration (CMC) values below 1 µM being observed in some cases. The reported micelles were found to solubilize hydrophobic metal complexes and organic molecules, suggesting their potential suitability for catalysis and drug delivery applications.

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