DNA Polymerase-Mediated Synthesis of Unbiased Threose Nucleic Acid (TNA) Polymers Requires 7-Deazaguanine To Suppress G:G Mispairing during TNA Transcription

2015 ◽  
Vol 137 (12) ◽  
pp. 4014-4017 ◽  
Author(s):  
Matthew R. Dunn ◽  
Andrew C. Larsen ◽  
Walter J. Zahurancik ◽  
Nour Eddine Fahmi ◽  
Madeline Meyers ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Polymerase ◽  
Threose Nucleic Acid

scholarly journals Schnellnachweis von SARS-CoV-2 mit recombinase polymerase amplification

BIOspektrum ◽  
2020 ◽  
Vol 26 (6) ◽  
pp. 624-627
Author(s):  
Ole Behrmann ◽  
Iris Bachmann ◽  
Frank Hufert ◽  
Gregory Dame
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Dna Polymerase ◽  
Fluorescent Probes ◽  
Reverse Transcription ◽  
Nucleic Acid Detection ◽  
Detection Scheme ◽  
Recombination Proteins ◽  
Target Dna ◽  
Rapid Amplification

Abstract The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39–42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.

Characterization of the nucleic acid product of the visna virus RNA dependent DNA polymerase

Virology ◽  
1974 ◽  
Vol 57 (1) ◽  
pp. 251-258 ◽  
Author(s):  
Ashley T. Haase ◽  
Axel C. Garapin ◽  
Anthony J. Faras ◽  
Harold E. Varmus ◽  
J.Michael Bishop
Keyword(s):  
Nucleic Acid ◽  
Dna Polymerase ◽  
Acid Product ◽  
Visna Virus ◽  
Virus Rna

A parallel stranded G-quadruplex composed of threose nucleic acid (TNA)

Biopolymers ◽  
2016 ◽  
Vol 107 (3) ◽  
pp. e22999 ◽  
Author(s):  
Jen-yu Liao ◽  
Irina Anosova ◽  
Saikat Bala ◽  
Wade D. Van Horn ◽  
John C. Chaput
Keyword(s):  
Nucleic Acid ◽  
G Quadruplex ◽  
Threose Nucleic Acid

scholarly journals Activation of innate immune responses by a CpG oligonucleotide sequence composed entirely of threose nucleic acid

2018 ◽  
Author(s):  
Margaret J. Lange ◽  
Donald H. Burke ◽  
John C. Chaput
Keyword(s):  
Nucleic Acid ◽  
Immune Responses ◽  
B Cell ◽  
Cellular Proliferation ◽  
Darwinian Evolution ◽  
Nucleic Acid Therapeutics ◽  
Dna And Rna ◽  
Cpg Oligonucleotide ◽  
Threose Nucleic Acid ◽  
Oligonucleotide Sequence

AbstractRecent advances in synthetic biology have led to the development of nucleic acid polymers with backbone structures distinct from those found in nature, termed xeno-nucleic acids (XNAs). Several unique properties of XNAs make them attractive as nucleic acid therapeutics, most notably their high resistance to serum nucleases and ability to form Watson-Crick base-pairing with DNA and RNA. The ability of XNAs to induce immune responses has not been investigated. Threose nucleic acid (TNA), a type of XNA, is recalcitrant to nuclease digestion and capable of undergoing Darwinian evolution to produce high affinity aptamers; thus, TNA is an attractive candidate for diverse applications, including nucleic acid therapeutics. Here, we evaluated a TNA oligonucleotide derived from a CpG oligonucleotide sequence known to activate TLR9-dependent immune signaling in B cell lines. We observed a slight induction of relevant mRNA signals, robust B cell line activation, and negligible effects on cellular proliferation.

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